Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 116322 |
| Name | oriT_pUSA03  |
| Organism | Staphylococcus aureus strain BAA-1556_Catania |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP136529 (34223..34258 [+], 36 nt) |
| oriT length | 36 nt |
| IRs (inverted repeats) | 4..11, 13..20 (GCGAACGT..ACGTTCGC) |
| Location of nic site | 29..30 |
| Conserved sequence flanking the nic site |
GTGCGCCCTT |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 36 nt
>oriT_pUSA03
CACGCGAACGTAACGTTCGCATAAGTGCGCCCTTAC
CACGCGAACGTAACGTTCGCATAAGTGCGCCCTTAC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 10429 | GenBank | WP_011443608 |
| Name | MobA_MobL_RZS17_RS14645_pUSA03 |
UniProt ID | _ |
| Length | 303 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 303 a.a. Molecular weight: 35855.17 Da Isoelectric Point: 9.4972
>WP_011443608.1 MobA/MobL family protein, partial [Staphylococcus aureus]
MAMYHFQNKFVSKANGQSATAKSAYNSASRIKDFKENEFKDYSNKQCDYSEILLPNNADDKFKDREYLWN
KVHDVENRKNSQVAREIIIGLPNEFDPNSNIELAKEFAESLSNEGMIVDLNIHKINEENPHAHLLCTLRG
LDKNNEFEPKRKGNDYIRDWNTKEKHNEWRKRWENVQNKHLEKNGFSVRVSADSYKNQNIDLEPTKKEGW
KARKFEDETGKKSSISKHNESIKKRNQQKIDKMFNEVDDMKSHKLNAFSYMNKSDSTTLKNMAKDLKIYV
TPINMYKENERLYDLKQKNFINH
MAMYHFQNKFVSKANGQSATAKSAYNSASRIKDFKENEFKDYSNKQCDYSEILLPNNADDKFKDREYLWN
KVHDVENRKNSQVAREIIIGLPNEFDPNSNIELAKEFAESLSNEGMIVDLNIHKINEENPHAHLLCTLRG
LDKNNEFEPKRKGNDYIRDWNTKEKHNEWRKRWENVQNKHLEKNGFSVRVSADSYKNQNIDLEPTKKEGW
KARKFEDETGKKSSISKHNESIKKRNQQKIDKMFNEVDDMKSHKLNAFSYMNKSDSTTLKNMAKDLKIYV
TPINMYKENERLYDLKQKNFINH
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 12175 | GenBank | WP_000209436 |
| Name | t4cp2_RZS17_RS14575_pUSA03 |
UniProt ID | _ |
| Length | 546 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 546 a.a. Molecular weight: 62672.60 Da Isoelectric Point: 7.6569
>WP_000209436.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Bacilli]
MTTLLGELESKATSINKKNLVIQDFDTKCPNRNIFVVGGPGSYKSAGYVIPNVIVKNQQSIVVTDPSGEV
YEKTSNIKRMQGFDVRVVNFKNFLASDRQNFFDYIDKDSDCSIVAELIIKSAGDSKINKDVWYKASVGLL
NSLLLYAKYEFEPEKRTIGNIIKFIQNNKPNQDEEGSVELDKRFNELSKDHPARESYEFGFAVSEGRTRA
SIILTFVADLRNYIDNEIRSYTSDSDFDLRDVGLRKTIIYVMLPVLGNTVQSLSSLFFSQMFQQLYRLGD
ENGARLPVPVDFLLDEWPNIGAIPDYEETLATCRKYGISITTIVQSISQAVDKYNKDKANAIIGNHAVIL
CLGNVNNDTAKYIKEELGNATVEFETSSEGSSSGKDSRSKSSNKNVSYTGRALLNEDEISNMQQDKAILI
TKNRNPKIIKKLAYFKMFPNLTETYIAPQNEYKRKKNQSMIDKHNRLREEWDKKQEKNKQQKLEEYKEEQ
RQKELEKEQQPQEEQQNEKVKESKDKNDQEDKKDEQQKINDSKKAFYEKLKQKQAK
MTTLLGELESKATSINKKNLVIQDFDTKCPNRNIFVVGGPGSYKSAGYVIPNVIVKNQQSIVVTDPSGEV
YEKTSNIKRMQGFDVRVVNFKNFLASDRQNFFDYIDKDSDCSIVAELIIKSAGDSKINKDVWYKASVGLL
NSLLLYAKYEFEPEKRTIGNIIKFIQNNKPNQDEEGSVELDKRFNELSKDHPARESYEFGFAVSEGRTRA
SIILTFVADLRNYIDNEIRSYTSDSDFDLRDVGLRKTIIYVMLPVLGNTVQSLSSLFFSQMFQQLYRLGD
ENGARLPVPVDFLLDEWPNIGAIPDYEETLATCRKYGISITTIVQSISQAVDKYNKDKANAIIGNHAVIL
CLGNVNNDTAKYIKEELGNATVEFETSSEGSSSGKDSRSKSSNKNVSYTGRALLNEDEISNMQQDKAILI
TKNRNPKIIKKLAYFKMFPNLTETYIAPQNEYKRKKNQSMIDKHNRLREEWDKKQEKNKQQKLEEYKEEQ
RQKELEKEQQPQEEQQNEKVKESKDKNDQEDKKDEQQKINDSKKAFYEKLKQKQAK
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 11407..22628
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| RZS17_RS14505 (RZS17_14505) | 6681..7157 | + | 477 | WP_000664727 | replication/maintenance protein RepL | - |
| RZS17_RS14510 (RZS17_14510) | 7865..8599 | + | 735 | WP_001003260 | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(C) | - |
| RZS17_RS14515 (RZS17_14515) | 8896..9570 | + | 675 | WP_001106019 | IS6-like element IS257 family transposase | - |
| RZS17_RS14520 (RZS17_14520) | 9679..9867 | - | 189 | WP_001063224 | hypothetical protein | - |
| RZS17_RS14525 (RZS17_14525) | 10039..11013 | + | 975 | WP_000368849 | conjugative transfer protein TrsA | - |
| RZS17_RS14530 (RZS17_14530) | 11030..11347 | + | 318 | WP_000591622 | CagC family type IV secretion system protein | - |
| RZS17_RS14535 (RZS17_14535) | 11407..11814 | + | 408 | WP_000110157 | hypothetical protein | trsC |
| RZS17_RS14540 (RZS17_14540) | 11801..12484 | + | 684 | WP_000979860 | TrsD/TraD family conjugative transfer protein | trsD |
| RZS17_RS14545 (RZS17_14545) | 12505..14517 | + | 2013 | WP_000874919 | VirB4 family type IV secretion system protein | virb4 |
| RZS17_RS14550 (RZS17_14550) | 14529..15809 | + | 1281 | WP_000702361 | membrane protein | trsF |
| RZS17_RS14555 (RZS17_14555) | 15827..16903 | + | 1077 | WP_000269385 | CHAP domain-containing protein | trsG |
| RZS17_RS14560 (RZS17_14560) | 16913..17398 | + | 486 | WP_000735569 | TrsH/TraH family protein | - |
| RZS17_RS14565 (RZS17_14565) | 17414..19516 | + | 2103 | WP_001094109 | DNA topoisomerase III | - |
| RZS17_RS14570 (RZS17_14570) | 19532..19996 | + | 465 | WP_000715273 | hypothetical protein | trsJ |
| RZS17_RS14575 (RZS17_14575) | 19993..21633 | + | 1641 | WP_000209436 | VirD4-like conjugal transfer protein, CD1115 family | - |
| RZS17_RS14580 (RZS17_14580) | 21711..22628 | + | 918 | WP_000383804 | conjugal transfer protein TrbL family protein | trsL |
| RZS17_RS14585 (RZS17_14585) | 22645..23037 | + | 393 | WP_000608970 | single-stranded DNA-binding protein | - |
| RZS17_RS14590 (RZS17_14590) | 23094..23261 | + | 168 | WP_000869301 | hypothetical protein | - |
| RZS17_RS14595 (RZS17_14595) | 23299..23973 | + | 675 | WP_001105950 | IS6-like element IS257 family transposase | - |
| RZS17_RS14600 (RZS17_14600) | 24103..24717 | + | 615 | WP_000527296 | hypothetical protein | - |
| RZS17_RS14605 (RZS17_14605) | 24786..26057 | + | 1272 | WP_000386736 | hypothetical protein | - |
| RZS17_RS14610 (RZS17_14610) | 26099..26515 | - | 417 | WP_000691761 | YolD-like family protein | - |
| RZS17_RS14615 (RZS17_14615) | 26810..27367 | + | 558 | WP_001273859 | recombinase family protein | - |
Host bacterium
| ID | 16755 | GenBank | NZ_CP136529 |
| Plasmid name | pUSA03 | Incompatibility group | - |
| Plasmid size | 37136 bp | Coordinate of oriT [Strand] | 34223..34258 [+] |
| Host baterium | Staphylococcus aureus strain BAA-1556_Catania |
Cargo genes
| Drug resistance gene | mupA, erm(C) |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIA21 |