Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 116310
• Name: oriT_pAt2
• Organism: Agrobacterium tumefaciens strain Teh001
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP048506 (102911..102951 [+], 41 nt)
• oriT length: 41 nt
• IRs (inverted repeats): 18..24, 29..35 (ACGTCGC..GCGACGT)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 41 nt

>oriT_pAt2
TCCAAGGGCGCAATTATACGTCGCTGATGCGACGTGCTTGC

oriT secondary structure

Predicted by RNAfold.

Relaxase

traA_FY147_RS28095_pAt2

• ID: 10422
• GenBank: WP_173995559
• Name: traA_FY147_RS28095_pAt2
• UniProt ID: _
• Length: 1100 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 1100 a.a.
• Molecular weight: 123269.13 Da
• Isoelectric Point: 9.9132

>WP_173995559.1 MULTISPECIES: Ti-type conjugative transfer relaxase TraA [Rhizobium/Agrobacterium group]
MAIAHFSASIVSRGSGRSVVLSAAYRHCAKMEYEREARTIDYTRKQGLVHEEFMLPADAPKWVRAMIADR
SVAGASEAFWNKVEAFEKRADAQLARDLTIALPLELPAEQNIALVRDFVEKHILAKGMVADWVYHDNPGN
PHIHLMTTLRPLTGDGFGTKKVAVIGEDGQPVRTKSGKILYELWAGSTDDFNVLRDGWFERLNHHLALGG
IDLRIDGRSYEKQGIDLEPTIHLGVGAKAIERKAKEQGIRPELERIELNEDRRSENARRILRRPEIVLDL
ITREKSVFDERDVAKVLHRYVDDPALFQQLMLRIILNPEVLRLQRDTIEFATGEKVPARYSTRAMIRLEA
TMARQAMWLSDKETHAVSTAVLAATFGRHGRLSEEQKAAIECIAGPARIAAVVGRAGAGKTTMMKAAREA
WELAGYRVVGGALAGKAAEGLEKEAGIHSRTLASWELRWNRGRDVLDRKTVFVMDEAGMVASKQMARFVD
TAVRAGAKIVLVGDPEQLQPIEAGAAFRAIVDRIGYAELETIYRQREDWMRKASLDLARGNVEKALTAYD
ANVRITGTRLKAEAVERLIGDWNHDYDQTKTTLILAHLRRDVRMLNVMAREKFVERGVVGEGHVFKTADG
IRQFDAGDQIVFLKNETSLGVKNGMIAHVVEAAPNRIVAVVGEGDKRRQVIVEQRFYSNLDHGYATTIHK
SQGATVDRVKVLASLSLDRHLTYVAMTRHREDVQLYYGRRSFAFNGGLAKVLSRKNAKETTLDYERGKLY
REALRFAEGRGLNIVQVARTLVRDRLDWTLRQKMKLVELGQRLAVFAEHLGLHHPLKTQTMKEAAPMVAG
IKTFSGSVADTLSDRLGADPALKQQWEEVSARFAYVFADPETAFRAMNFDAVLADKDSARQALQKLEAEP
TSIGALTGKTGILASKTEREARRIAELNVPALKRDLEQYLRMRETASQRLQADEQALRQRVSIDIPALSP
AACVVLERVRDAIDRNDLPAALGYALSNRETKLEIDGFNKAVTERFGERTLLTNAARQPSGTLFDKLSNG
MQPAQKEQLREAWPVMRTAQQIAAHERTTQTLKQAEELRLTQRQSPVMKQ

Protein domains

Predicted by InterproScan.

• BID domain of Bartonella effector protein (Bep) (Bep_C_terminal) (855-951)

• Viral (Superfamily 1) RNA helicase (Viral_helicase1) (692-736)

• MobA/MobL family (MobA_MobL) (17-243)

• AAA domain (AAA_30) (382-567)

Protein structure

No available structure.

Host bacterium

• ID: 16743
• GenBank: NZ_CP048506
• Plasmid name: pAt2
• Incompatibility group: -
• Plasmid size: 146234 bp
• Coordinate of oriT [Strand]: 102911..102951 [+]
• Host baterium: Agrobacterium tumefaciens strain Teh001

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -