Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   116309
Name   oriT_pAt2 in_silico
Organism   Agrobacterium tumefaciens strain Sut002
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP048512 (103332..103372 [+], 41 nt)
oriT length   41 nt
IRs (inverted repeats)      18..24, 29..35  (ACGTCGC..GCGACGT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 41 nt

>oriT_pAt2
TCCAAGGGCGCAATTATACGTCGCTGATGCGACGTGCTTGC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   10421 GenBank   WP_173995559
Name   traA_FY146_RS28110_pAt2 insolico UniProt ID   _
Length   1100 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1100 a.a.        Molecular weight: 123269.13 Da        Isoelectric Point: 9.9132

>WP_173995559.1 MULTISPECIES: Ti-type conjugative transfer relaxase TraA [Rhizobium/Agrobacterium group]
MAIAHFSASIVSRGSGRSVVLSAAYRHCAKMEYEREARTIDYTRKQGLVHEEFMLPADAPKWVRAMIADR
SVAGASEAFWNKVEAFEKRADAQLARDLTIALPLELPAEQNIALVRDFVEKHILAKGMVADWVYHDNPGN
PHIHLMTTLRPLTGDGFGTKKVAVIGEDGQPVRTKSGKILYELWAGSTDDFNVLRDGWFERLNHHLALGG
IDLRIDGRSYEKQGIDLEPTIHLGVGAKAIERKAKEQGIRPELERIELNEDRRSENARRILRRPEIVLDL
ITREKSVFDERDVAKVLHRYVDDPALFQQLMLRIILNPEVLRLQRDTIEFATGEKVPARYSTRAMIRLEA
TMARQAMWLSDKETHAVSTAVLAATFGRHGRLSEEQKAAIECIAGPARIAAVVGRAGAGKTTMMKAAREA
WELAGYRVVGGALAGKAAEGLEKEAGIHSRTLASWELRWNRGRDVLDRKTVFVMDEAGMVASKQMARFVD
TAVRAGAKIVLVGDPEQLQPIEAGAAFRAIVDRIGYAELETIYRQREDWMRKASLDLARGNVEKALTAYD
ANVRITGTRLKAEAVERLIGDWNHDYDQTKTTLILAHLRRDVRMLNVMAREKFVERGVVGEGHVFKTADG
IRQFDAGDQIVFLKNETSLGVKNGMIAHVVEAAPNRIVAVVGEGDKRRQVIVEQRFYSNLDHGYATTIHK
SQGATVDRVKVLASLSLDRHLTYVAMTRHREDVQLYYGRRSFAFNGGLAKVLSRKNAKETTLDYERGKLY
REALRFAEGRGLNIVQVARTLVRDRLDWTLRQKMKLVELGQRLAVFAEHLGLHHPLKTQTMKEAAPMVAG
IKTFSGSVADTLSDRLGADPALKQQWEEVSARFAYVFADPETAFRAMNFDAVLADKDSARQALQKLEAEP
TSIGALTGKTGILASKTEREARRIAELNVPALKRDLEQYLRMRETASQRLQADEQALRQRVSIDIPALSP
AACVVLERVRDAIDRNDLPAALGYALSNRETKLEIDGFNKAVTERFGERTLLTNAARQPSGTLFDKLSNG
MQPAQKEQLREAWPVMRTAQQIAAHERTTQTLKQAEELRLTQRQSPVMKQ

  Protein domains


Predicted by InterproScan.

BID domain of Bartonella effector protein (Bep) (Bep_C_terminal)

(855-951)

Viral (Superfamily 1) RNA helicase (Viral_helicase1)

(692-736)

MobA/MobL family (MobA_MobL)

(17-243)

AAA domain (AAA_30)

(382-567)


  Protein structure



No available structure.




T4CP

ID   12167 GenBank   WP_173995561
Name   traG_FY146_RS28095_pAt2 insolico UniProt ID   _
Length   658 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 658 a.a.        Molecular weight: 71160.44 Da        Isoelectric Point: 9.0431

>WP_173995561.1 Ti-type conjugative transfer system protein TraG [Agrobacterium tumefaciens]
MTMNRLLLLILPAIIMLAAMFATSGMEQRLAAFGTSPRAKLMLGHAGLALPYIAAVAIGIIGLFAANGSA
NIKAAGLSVLAGNGVVITTATLREVIRLNSIASRVPAEQSVLAYADPVTMIGVGIAFISGMFALRVAIKG
NAAFSTTAPKRIGGKRAVHGEADWMKIQETAKLFPEPGGIVIGERYRVDRDSVAAMPFRADDRQSWGAGG
KSPLLCFDGSFGSSHGIVFAGSGGFKTTSVTIPTALKWGGGLVVLDPSSEVAPMVCEHRRQAGRKVIVLD
PTAGGVGFNALDWIGRHGNTKEEDIVAVATWIMTDNPRTASARDDFFRASAMQLLTALIADVCLSGHTEA
EDQTLRRVRANLSEPEPKLRARLTKIYEGSESDFVKENVSVFVNMTPETFSGVYANAVKETHWLSYPNYA
GLVSGDSFSTDDLADGGTDIFIALDLKVLEAHPGLARVVIGSLLNAIYNRNGNVEGRTLFLLDEVARLGY
LRILETARDAGRKYGIMLTMIFQSLGQMREAYGGRDATSKWFESASWISFAAINDPDTADYISKRCGDTT
VEVDQTNRSTGMKGSSRSRSKQLSRRPLILPHEVLRMRSDEQIVFTSGNPPLRCGRAIWFRRDDMKACVG
ENRFHRTGTGTDTHTEHAPPWQKEGTRP

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(163-636)

  Protein structure



No available structure.



ID   12168 GenBank   WP_173995645
Name   t4cp2_FY146_RS28420_pAt2 insolico UniProt ID   _
Length   822 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 822 a.a.        Molecular weight: 92049.83 Da        Isoelectric Point: 5.5826

>WP_173995645.1 MULTISPECIES: conjugal transfer protein TrbE [Rhizobium/Agrobacterium group]
MVALKLFRHSGPSFADLVPYAGLVDSGIILLKDGSLMAGWYFAGPDSESSTDAERNEVSRQINLILSRLG
SGWMIQVEALRVPTIDYPAGDICHFPDPVTRAIDTERRSHFERESGHFESRHALILTWRPPEPRRSGLTR
YVYSDTASRSATYADTALDTFRTSIREVEQYLANVLSIRRMVTRETEERGGFRVARYDELFQFIRFCITG
ENHPVRLPDIPMYLDWLVTAELQHGLTPTVENRFLGVVAIDGLPAESWPGILNSLDLMPLTYRWSSRFVF
LDEQEARQKLERTRKKWQQKVRPFFDQLFQTQSRSLDQDAMMMVAETEDAIAEASSQLVAYGYYTPVIVL
FDEQQERLQEKCEAIRRLIQAEGFGARIETLNATDAFLGSLPGVSYANIREPLVNTRNLADLIPLNSVWS
GSPVAPCPFYPPGSPPLMQVASGSTPFRLNLHVDDVGHTLVFGPTGSGKSTLLSLIAAQFRRYADAQIFA
FDKGRSMLPLTLAIGGDHYEIGGDAAEGGEGDSPRLAFCPLAELASDGDRAWAAEWIETLVALQGVTVTP
DYRNAISRQLALMAESRGRSLSDFVSGVQMREIKDALHHYTVDGPMGQLLDAEQDGLALGAFQCFEVEEL
MNLGERNLVPVLLYMFRRVEKRLTGAPSLIILDEAWLMLGHPTFRNKIREWLKVLRKANCAVVLATQSIS
DAERSGIIDVLKESCPTKICLPNGAAREPGTREFYERIGFNERQIEIVATALPKREYYVASPEGRRLFDM
SLGPVALSFVGASGKEDLKRIRALHFEHGADWPKHWLQQRGIAHAETLFQAA

  Protein domains


Predicted by InterproScan.

CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB)

(188-400)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 164393..174065

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FY146_RS28360 (FY146_28155) 159677..160402 - 726 WP_173995637 nopaline ABC transporter permease NocM -
FY146_RS28365 (FY146_28160) 160404..161114 - 711 WP_173995654 nopaline ABC transporter permease NocQ -
FY146_RS28370 (FY146_28165) 161188..162039 - 852 WP_173995638 nopaline ABC transporter substrate-binding protein NocT -
FY146_RS28375 (FY146_28170) 162104..162877 - 774 WP_173995639 nopaline ABC transporter ATP-binding protein NocP -
FY146_RS28380 (FY146_28175) 163131..164033 + 903 WP_045023359 LysR family transcriptional regulator -
FY146_RS28385 (FY146_28180) 164393..165700 - 1308 WP_174168418 IncP-type conjugal transfer protein TrbI virB10
FY146_RS28390 (FY146_28185) 165715..166191 - 477 WP_045025162 conjugal transfer protein TrbH -
FY146_RS28395 (FY146_28190) 166191..167045 - 855 WP_173995641 P-type conjugative transfer protein TrbG virB9
FY146_RS28400 (FY146_28195) 167063..167725 - 663 WP_060716494 conjugal transfer protein TrbF virB8
FY146_RS28405 (FY146_28200) 167740..168936 - 1197 WP_173995642 P-type conjugative transfer protein TrbL virB6
FY146_RS28410 (FY146_28205) 168930..169148 - 219 WP_173995643 entry exclusion protein TrbK -
FY146_RS28415 (FY146_28210) 169145..169909 - 765 Protein_153 P-type conjugative transfer protein TrbJ -
FY146_RS28420 (FY146_28215) 169926..172394 - 2469 WP_173995645 conjugal transfer protein TrbE virb4
FY146_RS28425 (FY146_28220) 172405..172704 - 300 WP_173995646 conjugal transfer protein TrbD virB3
FY146_RS28430 (FY146_28225) 172697..173104 - 408 WP_173995647 conjugal transfer pilin TrbC virB2
FY146_RS28435 (FY146_28230) 173094..174065 - 972 WP_173995648 P-type conjugative transfer ATPase TrbB virB11
FY146_RS28440 (FY146_28235) 174062..174697 - 636 WP_173995649 acyl-homoserine-lactone synthase -

Host bacterium

ID   16742 GenBank   NZ_CP048512
Plasmid name   pAt2 Incompatibility group   -
Plasmid size   175062 bp Coordinate of oriT [Strand]   103332..103372 [+]
Host baterium   Agrobacterium tumefaciens strain Sut002

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -