Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 116281 |
| Name | oriT_pWS-31  |
| Organism | Staphylococcus warneri strain WS479 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP061042 (26494..26531 [+], 38 nt) |
| oriT length | 38 nt |
| IRs (inverted repeats) | 14..22, 29..37 (TAAAGTATA..TATACTTTA) 2..8, 13..19 (ACTTTAT..ATAAAGT) |
| Location of nic site | 27..28 |
| Conserved sequence flanking the nic site |
GTGTGTTATA |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 38 nt
>oriT_pWS-31
CACTTTATGAATATAAAGTATAGTGTGTTATACTTTAC
CACTTTATGAATATAAAGTATAGTGTGTTATACTTTAC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 10415 | GenBank | WP_000119405 |
| Name | mobV_IAR39_RS12475_pWS-31 |
UniProt ID | F5X340 |
| Length | 420 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 420 a.a. Molecular weight: 49660.33 Da Isoelectric Point: 9.9693
>WP_000119405.1 MULTISPECIES: MobV family relaxase [Bacteria]
MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENYDLKNDKNIDYNERVKEIIESQKTGTR
KTRKDAVLVNELLVTSDRDFFEQLDPGEQKRFFEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPM
RDGKLQGKNVFNRQELLWLQDKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVK
KDEWTAYSDKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTAARDN
DRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEENKSLKSKISDLKRDVSL
IYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHDLEPKKNEFELTHNREVKKERSRDQGMSL
MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENYDLKNDKNIDYNERVKEIIESQKTGTR
KTRKDAVLVNELLVTSDRDFFEQLDPGEQKRFFEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPM
RDGKLQGKNVFNRQELLWLQDKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVK
KDEWTAYSDKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTAARDN
DRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEENKSLKSKISDLKRDVSL
IYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHDLEPKKNEFELTHNREVKKERSRDQGMSL
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | F5X340 |
T4CP
| ID | 12157 | GenBank | WP_000125496 |
| Name | tcpA_IAR39_RS12385_pWS-31 |
UniProt ID | _ |
| Length | 358 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 358 a.a. Molecular weight: 40500.67 Da Isoelectric Point: 5.5890
>WP_000125496.1 MULTISPECIES: hypothetical protein [Staphylococcus]
MTAKELEQYLLQSLNMALGSMMQGETSYTNSFTCKVTEEGFYFLPRLPAGYIVDDDLYQKIFLIASAALY
PKFTLLKQNSAYFVPMDTDDIHTQRALFFPWQQGISERLVISDLDRFTSSLTNQQIPIMKQLTLNYQNIT
SIAIAGNSGSGKSYMLTYLLSVLKPISELIIVDPKFDTPSRWARIHDITVIHPHQNRSKSDFLSEINENL
SHCLQVIQQRQTILYDDPDYQFDHLTIVIDEVLALAEGVNKTIKDAFFSLLSQIALLGRATKVHLLLVSQ
RFDHQTIPVSVREQLNVLIQIGNINKKTTQFLFPDLDPEGIVIPIGKGTGLIQVIDSESPYQVLPLLCPT
YYTKAGVL
MTAKELEQYLLQSLNMALGSMMQGETSYTNSFTCKVTEEGFYFLPRLPAGYIVDDDLYQKIFLIASAALY
PKFTLLKQNSAYFVPMDTDDIHTQRALFFPWQQGISERLVISDLDRFTSSLTNQQIPIMKQLTLNYQNIT
SIAIAGNSGSGKSYMLTYLLSVLKPISELIIVDPKFDTPSRWARIHDITVIHPHQNRSKSDFLSEINENL
SHCLQVIQQRQTILYDDPDYQFDHLTIVIDEVLALAEGVNKTIKDAFFSLLSQIALLGRATKVHLLLVSQ
RFDHQTIPVSVREQLNVLIQIGNINKKTTQFLFPDLDPEGIVIPIGKGTGLIQVIDSESPYQVLPLLCPT
YYTKAGVL
Protein domains
No domain identified.
Protein structure
No available structure.
Host bacterium
| ID | 16714 | GenBank | NZ_CP061042 |
| Plasmid name | pWS-31 | Incompatibility group | - |
| Plasmid size | 31000 bp | Coordinate of oriT [Strand] | 26494..26531 [+] |
| Host baterium | Staphylococcus warneri strain WS479 |
Cargo genes
| Drug resistance gene | msr(A), aadD, blaZ |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIA21 |