Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 116277 |
| Name | oriT_pHL24830  |
| Organism | Staphylococcus aureus strain HL24830 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP082790 (21921..21956 [-], 36 nt) |
| oriT length | 36 nt |
| IRs (inverted repeats) | 4..10, 14..20 (GCGAACG..CGTTCGC) |
| Location of nic site | 29..30 |
| Conserved sequence flanking the nic site |
GTGCGCCCTT |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 36 nt
>oriT_pHL24830
CACGCGAACGGAACGTTCGCATAAGTGCGCCCTTAC
CACGCGAACGGAACGTTCGCATAAGTGCGCCCTTAC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 10412 | GenBank | WP_223065265 |
| Name | MobA_MobL_K3B00_RS14635_pHL24830 |
UniProt ID | _ |
| Length | 665 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 665 a.a. Molecular weight: 78923.96 Da Isoelectric Point: 5.5428
>WP_223065265.1 MobA/MobL family protein [Staphylococcus aureus]
MAMYHFQNKFVSKANGQSATAKSAYNSASRIKDFKENEFKDYSNKQCDYSEILLPNNADDKFKDREYLWN
KVHDVENRKNSQVAREIIIGLPNEFDPNSNIELAKEFAESLSNEGMIVDLNIHKINEENPHAHLLCTLRG
LDKNNEFEPKRKGNDYIRDWNTKEKHNEWRKRWENVQNKHLEKNGFSVRVSADSYKNQNIDLEPTKKEGW
KARKFEDETGKKSSISKHNESIKKRNQQKIDKMFNEVDDMKSHKLNAFSYMNKSDSTTLKNMAKDLKIYV
TPINMYKENERLYDLKQKTSLITDDEDRLNKIEDIEDRQKKLESINEVFEKQAGIFFDKNYPDQSLNYSD
DEKIFITRTILNDRDVLPANNELEDIVKEKRIKEAQISLNTVLGNRDISLESIEKESNFFADKLSNILEK
NNLSFDDVLENKHEGMEDSLKIDYYTNKLEVFRNAKNILEDYYDVQIKELFTDDEDYKAFNEVTDIKEKQ
QLIDFKTYHGTENTIEMLETGNFIPKYSDEDRKYITEQVKLLQEKEFKPNKNQHDKFVFGAIQKKLLSEY
DFDYSDNNDLKHLYQESNEVGDEISKDNIEEFYEGNDILVDKETYNNYNRKQQAYGLINAGLDSMIFNFN
EIFRERMLKYINHQYKGKNHSKQRHELKNKRGMHL
MAMYHFQNKFVSKANGQSATAKSAYNSASRIKDFKENEFKDYSNKQCDYSEILLPNNADDKFKDREYLWN
KVHDVENRKNSQVAREIIIGLPNEFDPNSNIELAKEFAESLSNEGMIVDLNIHKINEENPHAHLLCTLRG
LDKNNEFEPKRKGNDYIRDWNTKEKHNEWRKRWENVQNKHLEKNGFSVRVSADSYKNQNIDLEPTKKEGW
KARKFEDETGKKSSISKHNESIKKRNQQKIDKMFNEVDDMKSHKLNAFSYMNKSDSTTLKNMAKDLKIYV
TPINMYKENERLYDLKQKTSLITDDEDRLNKIEDIEDRQKKLESINEVFEKQAGIFFDKNYPDQSLNYSD
DEKIFITRTILNDRDVLPANNELEDIVKEKRIKEAQISLNTVLGNRDISLESIEKESNFFADKLSNILEK
NNLSFDDVLENKHEGMEDSLKIDYYTNKLEVFRNAKNILEDYYDVQIKELFTDDEDYKAFNEVTDIKEKQ
QLIDFKTYHGTENTIEMLETGNFIPKYSDEDRKYITEQVKLLQEKEFKPNKNQHDKFVFGAIQKKLLSEY
DFDYSDNNDLKHLYQESNEVGDEISKDNIEEFYEGNDILVDKETYNNYNRKQQAYGLINAGLDSMIFNFN
EIFRERMLKYINHQYKGKNHSKQRHELKNKRGMHL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 12154 | GenBank | WP_000209436 |
| Name | t4cp2_K3B00_RS14540_pHL24830 |
UniProt ID | _ |
| Length | 546 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 546 a.a. Molecular weight: 62672.60 Da Isoelectric Point: 7.6569
>WP_000209436.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Bacilli]
MTTLLGELESKATSINKKNLVIQDFDTKCPNRNIFVVGGPGSYKSAGYVIPNVIVKNQQSIVVTDPSGEV
YEKTSNIKRMQGFDVRVVNFKNFLASDRQNFFDYIDKDSDCSIVAELIIKSAGDSKINKDVWYKASVGLL
NSLLLYAKYEFEPEKRTIGNIIKFIQNNKPNQDEEGSVELDKRFNELSKDHPARESYEFGFAVSEGRTRA
SIILTFVADLRNYIDNEIRSYTSDSDFDLRDVGLRKTIIYVMLPVLGNTVQSLSSLFFSQMFQQLYRLGD
ENGARLPVPVDFLLDEWPNIGAIPDYEETLATCRKYGISITTIVQSISQAVDKYNKDKANAIIGNHAVIL
CLGNVNNDTAKYIKEELGNATVEFETSSEGSSSGKDSRSKSSNKNVSYTGRALLNEDEISNMQQDKAILI
TKNRNPKIIKKLAYFKMFPNLTETYIAPQNEYKRKKNQSMIDKHNRLREEWDKKQEKNKQQKLEEYKEEQ
RQKELEKEQQPQEEQQNEKVKESKDKNDQEDKKDEQQKINDSKKAFYEKLKQKQAK
MTTLLGELESKATSINKKNLVIQDFDTKCPNRNIFVVGGPGSYKSAGYVIPNVIVKNQQSIVVTDPSGEV
YEKTSNIKRMQGFDVRVVNFKNFLASDRQNFFDYIDKDSDCSIVAELIIKSAGDSKINKDVWYKASVGLL
NSLLLYAKYEFEPEKRTIGNIIKFIQNNKPNQDEEGSVELDKRFNELSKDHPARESYEFGFAVSEGRTRA
SIILTFVADLRNYIDNEIRSYTSDSDFDLRDVGLRKTIIYVMLPVLGNTVQSLSSLFFSQMFQQLYRLGD
ENGARLPVPVDFLLDEWPNIGAIPDYEETLATCRKYGISITTIVQSISQAVDKYNKDKANAIIGNHAVIL
CLGNVNNDTAKYIKEELGNATVEFETSSEGSSSGKDSRSKSSNKNVSYTGRALLNEDEISNMQQDKAILI
TKNRNPKIIKKLAYFKMFPNLTETYIAPQNEYKRKKNQSMIDKHNRLREEWDKKQEKNKQQKLEEYKEEQ
RQKELEKEQQPQEEQQNEKVKESKDKNDQEDKKDEQQKINDSKKAFYEKLKQKQAK
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 4132..15353
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| K3B00_RS14500 (K3B00_002889) | 1..333 | + | 333 | Protein_0 | IS256 family transposase | - |
| K3B00_RS14505 (K3B00_002890) | 463..1902 | - | 1440 | WP_001028144 | aminoglycoside O-phosphotransferase APH(2'')-Ia | - |
| K3B00_RS14510 (K3B00_002891) | 1903..2295 | - | 393 | WP_000393259 | GNAT family N-acetyltransferase | - |
| K3B00_RS14515 (K3B00_002892) | 2352..2726 | - | 375 | Protein_3 | IS256 family transposase | - |
| K3B00_RS14520 (K3B00_002893) | 2787..3461 | - | 675 | WP_001105984 | IS6-like element IS257 family transposase | - |
| K3B00_RS14525 (K3B00_002894) | 3499..3666 | - | 168 | WP_000869301 | hypothetical protein | - |
| K3B00_RS14530 (K3B00_002895) | 3723..4115 | - | 393 | WP_000608970 | single-stranded DNA-binding protein | - |
| K3B00_RS14535 (K3B00_002896) | 4132..5049 | - | 918 | WP_000383804 | conjugal transfer protein TrbL family protein | trsL |
| K3B00_RS14540 (K3B00_002897) | 5127..6767 | - | 1641 | WP_000209436 | VirD4-like conjugal transfer protein, CD1115 family | - |
| K3B00_RS14545 (K3B00_002898) | 6764..7228 | - | 465 | WP_000715273 | hypothetical protein | trsJ |
| K3B00_RS14550 (K3B00_002899) | 7244..9346 | - | 2103 | WP_001094109 | DNA topoisomerase III | - |
| K3B00_RS14555 (K3B00_002900) | 9362..9847 | - | 486 | WP_000735569 | TrsH/TraH family protein | - |
| K3B00_RS14560 (K3B00_002901) | 9857..10933 | - | 1077 | WP_000269385 | CHAP domain-containing protein | trsG |
| K3B00_RS14565 (K3B00_002902) | 10951..12231 | - | 1281 | WP_000702361 | membrane protein | trsF |
| K3B00_RS14570 (K3B00_002903) | 12243..14255 | - | 2013 | WP_000874919 | VirB4 family type IV secretion system protein | virb4 |
| K3B00_RS14575 (K3B00_002904) | 14276..14959 | - | 684 | WP_000979860 | TrsD/TraD family conjugative transfer protein | trsD |
| K3B00_RS14580 (K3B00_002905) | 14946..15353 | - | 408 | WP_000110157 | hypothetical protein | trsC |
| K3B00_RS14585 (K3B00_002906) | 15413..15730 | - | 318 | WP_223065267 | CagC family type IV secretion system protein | - |
| K3B00_RS14590 (K3B00_002907) | 15747..16721 | - | 975 | WP_000368849 | conjugative transfer protein TrsA | - |
| K3B00_RS14595 (K3B00_002908) | 16893..17081 | + | 189 | WP_001063224 | hypothetical protein | - |
| K3B00_RS14600 (K3B00_002909) | 17190..17864 | - | 675 | WP_001106019 | IS6-like element IS257 family transposase | - |
| K3B00_RS14605 (K3B00_002910) | 17983..18942 | - | 960 | WP_000043161 | replication initiator protein A | - |
| K3B00_RS14610 (K3B00_002911) | 19323..19583 | + | 261 | WP_001008213 | helix-turn-helix domain-containing protein | - |
| K3B00_RS14615 (K3B00_002912) | 19587..19916 | - | 330 | WP_000358311 | recombinase | - |
Host bacterium
| ID | 16710 | GenBank | NZ_CP082790 |
| Plasmid name | pHL24830 | Incompatibility group | - |
| Plasmid size | 38056 bp | Coordinate of oriT [Strand] | 21921..21956 [-] |
| Host baterium | Staphylococcus aureus strain HL24830 |
Cargo genes
| Drug resistance gene | aac(6')-aph(2''), mupA, qacD |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIA21 |