Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 116266 |
| Name | oriT_ES-397|unnamed1  |
| Organism | Enterococcus faecalis strain ES-397 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP136342 (27213..27262 [-], 50 nt) |
| oriT length | 50 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 50 nt
>oriT_ES-397|unnamed1
AATATCGCAACATGGTACCATGTTGCTCTGCTCGCAAAAAGAAAGCCTAC
AATATCGCAACATGGTACCATGTTGCTCTGCTCGCAAAAAGAAAGCCTAC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 10403 | GenBank | WP_002367932 |
| Name | Relaxase_RX142_RS15405_ES-397|unnamed1 |
UniProt ID | R3K157 |
| Length | 561 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 561 a.a. Molecular weight: 65400.15 Da Isoelectric Point: 6.0174
>WP_002367932.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Enterococcus]
MVYTKHFVIHTFDKLNNACSYIENAEKTEVTNDNPSEHLEHLFQYIVNDDKTYMKKLVSGHGIVDPTNPY
EEFKLTKLQAAIQRKIGYTFDPKSERLLPPTLTELEKGNAVLAHHLIQSFSPEDDLTPEKIHEIGYNTVM
ELTGGEYEFVIATHVDKEHLHNHIIFSSTNLKTGKAFRWQKGTKKVFEQISDKIAAKEGAKIIEKSPKNS
HKKYTMWETESLYKEKIKSRLDFLLEQSSSINDFLEKAEALNLSVDFSKKWTTYRLLDEPQIKNTRSRSL
SKSDPTRYNYEKIVERLKENKSVLTLKEVVEKYVEKSEKSKNEFDYQLTIDEWQISHKTTRGYYVNVDFG
FGERGKVFIGAYKVDPLENGQYNIYVKRKDFFYLMNEKNADRNRYMTGETLIKQLRLYNGQTPLKKEPVM
RTIDELVNAINFLAANEIEDTRQLELLEEKLEAAFLEAEETLETLDEKMLELHQLSNLLLENELNGELEL
IQQKLKTLLPDATLAEFSYEDVRGEIEAIKTSQSLLENKLERTRNEINQLHEIQAVQKKETANQNNVKPK
L
MVYTKHFVIHTFDKLNNACSYIENAEKTEVTNDNPSEHLEHLFQYIVNDDKTYMKKLVSGHGIVDPTNPY
EEFKLTKLQAAIQRKIGYTFDPKSERLLPPTLTELEKGNAVLAHHLIQSFSPEDDLTPEKIHEIGYNTVM
ELTGGEYEFVIATHVDKEHLHNHIIFSSTNLKTGKAFRWQKGTKKVFEQISDKIAAKEGAKIIEKSPKNS
HKKYTMWETESLYKEKIKSRLDFLLEQSSSINDFLEKAEALNLSVDFSKKWTTYRLLDEPQIKNTRSRSL
SKSDPTRYNYEKIVERLKENKSVLTLKEVVEKYVEKSEKSKNEFDYQLTIDEWQISHKTTRGYYVNVDFG
FGERGKVFIGAYKVDPLENGQYNIYVKRKDFFYLMNEKNADRNRYMTGETLIKQLRLYNGQTPLKKEPVM
RTIDELVNAINFLAANEIEDTRQLELLEEKLEAAFLEAEETLETLDEKMLELHQLSNLLLENELNGELEL
IQQKLKTLLPDATLAEFSYEDVRGEIEAIKTSQSLLENKLERTRNEINQLHEIQAVQKKETANQNNVKPK
L
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | R3K157 |
Auxiliary protein
| ID | 6115 | GenBank | WP_002367696 |
| Name | WP_002367696_ES-397|unnamed1 |
UniProt ID | Q82YL4 |
| Length | 118 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
Auxiliary protein sequence
Download Length: 118 a.a. Molecular weight: 14042.10 Da Isoelectric Point: 9.9724
>WP_002367696.1 MULTISPECIES: plasmid mobilization relaxosome protein MobC [Bacteria]
MENKQKLKRPIQRKIRFSKEENELINRKIEESFFPNFQNFALHLLIQGEIRHVDYSELNRLTTEIHKIGI
NINQMARLANQFHEISSEDIKDLTDKVQSLNALVQSELNKLIKRKEQS
MENKQKLKRPIQRKIRFSKEENELINRKIEESFFPNFQNFALHLLIQGEIRHVDYSELNRLTTEIHKIGI
NINQMARLANQFHEISSEDIKDLTDKVQSLNALVQSELNKLIKRKEQS
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | Q82YL4 |
T4CP
| ID | 12143 | GenBank | WP_077932944 |
| Name | t4cp2_RX142_RS15425_ES-397|unnamed1 |
UniProt ID | _ |
| Length | 609 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 609 a.a. Molecular weight: 70131.11 Da Isoelectric Point: 6.1811
>WP_077932944.1 VirD4-like conjugal transfer protein, CD1115 family [Enterococcus faecalis]
MQTYKKELKPYLYLGVFLFVIGFGIGNFFWLLPGTDYFSKTNYLFTKDILTYIEQTFYRFFITPSLLGFS
VGILGFLLGLLMYVRDNDRGIYRHGEEYGSARFATPAEMKKYEDPIPENNIIVSKHVKISLFNKRLPIKL
QKNKNIAILGDSGAAKTLAFIKTNLMQRHASFITTDPDGGILPEIGLLLKKGKYKIKVLDLNTLSNSDTF
NVFEYMHSELDVDRVLEAVTEATKEGEKTTGEDFWIKAEGLLVRSLIAYLWFDGRRNDYTPHLGMIADML
RHLKRKDKNVPSPVEEWFEELNEAIPDNYAYRQWTLFNNLYESETRASVLGIAAARYSVFDHEEVVNMIR
TDTMDIDSWNEEKTAVFIAIPETNTSFNFIAALMFATVGEVLRNKSDQVRLGKRVLEEGKELLHVRFLID
EFANIGRIPHFEKMLTTFRKREMSFTIVLQSLNQLQTLYRNGWQNILNGCACLLYLGGDEEETTKYLSAR
AGKQTLSIRKHSMNKGNQGGGSENRDKYGRDLIDRSEVALIGGDECLVFISKEHVFKDKKFYAFQHDRAD
ELATGPTDDKWYTWRRYMTDEEAILDKISATDIIDHGEITSELKEETHV
MQTYKKELKPYLYLGVFLFVIGFGIGNFFWLLPGTDYFSKTNYLFTKDILTYIEQTFYRFFITPSLLGFS
VGILGFLLGLLMYVRDNDRGIYRHGEEYGSARFATPAEMKKYEDPIPENNIIVSKHVKISLFNKRLPIKL
QKNKNIAILGDSGAAKTLAFIKTNLMQRHASFITTDPDGGILPEIGLLLKKGKYKIKVLDLNTLSNSDTF
NVFEYMHSELDVDRVLEAVTEATKEGEKTTGEDFWIKAEGLLVRSLIAYLWFDGRRNDYTPHLGMIADML
RHLKRKDKNVPSPVEEWFEELNEAIPDNYAYRQWTLFNNLYESETRASVLGIAAARYSVFDHEEVVNMIR
TDTMDIDSWNEEKTAVFIAIPETNTSFNFIAALMFATVGEVLRNKSDQVRLGKRVLEEGKELLHVRFLID
EFANIGRIPHFEKMLTTFRKREMSFTIVLQSLNQLQTLYRNGWQNILNGCACLLYLGGDEEETTKYLSAR
AGKQTLSIRKHSMNKGNQGGGSENRDKYGRDLIDRSEVALIGGDECLVFISKEHVFKDKKFYAFQHDRAD
ELATGPTDDKWYTWRRYMTDEEAILDKISATDIIDHGEITSELKEETHV
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 29906..48653
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| RX142_RS15405 (RX142_15405) | 25104..26789 | - | 1686 | WP_002367932 | relaxase/mobilization nuclease domain-containing protein | - |
| RX142_RS15410 (RX142_15410) | 26790..27146 | - | 357 | WP_002367696 | plasmid mobilization relaxosome protein MobC | - |
| RX142_RS15415 (RX142_15415) | 27392..27664 | - | 273 | WP_002362409 | LtrD | - |
| RX142_RS15420 (RX142_15420) | 27697..29856 | - | 2160 | WP_316499092 | ArdC-like ssDNA-binding domain-containing protein | - |
| RX142_RS15425 (RX142_15425) | 29906..31735 | - | 1830 | WP_077932944 | VirD4-like conjugal transfer protein, CD1115 family | virb4 |
| RX142_RS15430 (RX142_15430) | 31735..32220 | - | 486 | WP_002386493 | PcfB family protein | gbs1365 |
| RX142_RS15435 (RX142_15435) | 32262..32444 | - | 183 | WP_002367689 | hypothetical protein | - |
| RX142_RS15440 (RX142_15440) | 32490..32948 | - | 459 | WP_316499093 | single-stranded DNA-binding protein | - |
| RX142_RS15445 (RX142_15445) | 32965..33720 | - | 756 | WP_002425089 | Fic family protein | - |
| RX142_RS15450 (RX142_15450) | 33833..34441 | - | 609 | WP_002367686 | hypothetical protein | - |
| RX142_RS15455 (RX142_15455) | 34425..34670 | - | 246 | WP_002393743 | hypothetical protein | - |
| RX142_RS15460 (RX142_15460) | 34657..35283 | - | 627 | WP_010831027 | hypothetical protein | prgL |
| RX142_RS15465 (RX142_15465) | 35307..37922 | - | 2616 | WP_316499086 | phage tail tip lysozyme | prgK |
| RX142_RS15470 (RX142_15470) | 37934..40324 | - | 2391 | WP_010815738 | VirB4-like conjugal transfer ATPase, CD1110 family | virb4 |
| RX142_RS15475 (RX142_15475) | 40278..40631 | - | 354 | WP_002367919 | PrgI family mobile element protein | prgIc |
| RX142_RS15480 (RX142_15480) | 40633..41433 | - | 801 | WP_002367679 | type IV secretion system protein | prgHb |
| RX142_RS15485 (RX142_15485) | 41448..41744 | - | 297 | WP_172500140 | type III secretion system protein PrgG | - |
| RX142_RS15490 (RX142_15490) | 41757..41984 | - | 228 | WP_002365858 | hypothetical protein | - |
| RX142_RS15495 (RX142_15495) | 42031..42465 | - | 435 | WP_172500141 | type III secretion system protein PrgE | - |
| RX142_RS15500 (RX142_15500) | 42486..43382 | - | 897 | WP_172500142 | type III secretion system protein PrgD | - |
| RX142_RS15505 (RX142_15505) | 43424..44281 | - | 858 | WP_002367673 | pheromone response LPXTG-anchored adhesin PrgC | prgC |
| RX142_RS15510 (RX142_15510) | 44309..44665 | - | 357 | WP_002367672 | pheromone response system RNA-binding regulator PrgU | - |
| RX142_RS15515 (RX142_15515) | 44727..48653 | - | 3927 | WP_172500143 | LPXTG-anchored aggregation substance PrgB/Asc10 | prgB |
| RX142_RS15520 (RX142_15520) | 48879..49466 | - | 588 | WP_142430344 | hypothetical protein | - |
| RX142_RS15525 (RX142_15525) | 49535..52207 | - | 2673 | WP_172500144 | surface exclusion protein SEA1/PrgA | - |
| RX142_RS15530 (RX142_15530) | 52218..52523 | - | 306 | WP_010715949 | hypothetical protein | - |
| RX142_RS15535 (RX142_15535) | 52825..53046 | - | 222 | WP_002386480 | hypothetical protein | - |
| RX142_RS15540 (RX142_15540) | 53097..53477 | - | 381 | WP_172500145 | type III secretion system protein PrgR | - |
Host bacterium
| ID | 16699 | GenBank | NZ_CP136342 |
| Plasmid name | ES-397|unnamed1 | Incompatibility group | - |
| Plasmid size | 68344 bp | Coordinate of oriT [Strand] | 27213..27262 [-] |
| Host baterium | Enterococcus faecalis strain ES-397 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | prgB/asc10 |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIA9 |