Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 116264 |
| Name | oriT_ES-1348|unnamed1  |
| Organism | Enterococcus faecalis strain ES-1348 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP136352 (23973..24022 [-], 50 nt) |
| oriT length | 50 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 50 nt
>oriT_ES-1348|unnamed1
AATATCGCAACATGGTACCATGTTGCTCTGCTCGCAAAAAGAAAGCCTAC
AATATCGCAACATGGTACCATGTTGCTCTGCTCGCAAAAAGAAAGCCTAC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 10401 | GenBank | WP_316498399 |
| Name | Relaxase_RX144_RS14420_ES-1348|unnamed1 |
UniProt ID | _ |
| Length | 561 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 561 a.a. Molecular weight: 65340.14 Da Isoelectric Point: 6.1233
>WP_316498399.1 relaxase/mobilization nuclease domain-containing protein [Enterococcus faecalis]
MVYTKHFVIHTFDKLNNACSYIENAEKTEVTNDNPSEHLEHLFQYIVNDDKTYMKKLVSGHGIVDPTNPY
EEFKLTKLQAAIQRKIGYTFDPKSERLLPPTLTELEKGNAVLAHHLIQSFSPEDDLTPEKIHEIGYNTVM
ELTGGEYEFVIATHVDKEHLHNHIIFSSTNLKTGKAFRWQKGTKKVFEQISDKIAAKEGAKIIEKSPKNS
HKKYTMWETESLYKEKIKSRLDFLLEQSSSINDFLEKAEALNLSVDFSKKWTTYRLLDEPQIKNTRSRSL
SKSAPTRYNYEKIVERLKENKSVLTLKEVVEKYVEKSEKAKNEFDYQLTIDEWQISHKTTRGYYVNVDFG
FGERGKVFIGAYKVDPLENGQYNIYVKRKDFFYLMNEKNADRNRYMTGETLIKQLRLYNGQTPLKKEPVM
RTIDELVNAINFLAANEIEDTRQLELLEEKLEAAFLEAEETLETLDEKMLELHQLSNLLLENELNGELEL
IQQKLKTLLPDATLAEFSYEDVRGEIEAIKTSQSLLENKLERTRNEINQLHEIQAVQKKETANQNNVKPK
L
MVYTKHFVIHTFDKLNNACSYIENAEKTEVTNDNPSEHLEHLFQYIVNDDKTYMKKLVSGHGIVDPTNPY
EEFKLTKLQAAIQRKIGYTFDPKSERLLPPTLTELEKGNAVLAHHLIQSFSPEDDLTPEKIHEIGYNTVM
ELTGGEYEFVIATHVDKEHLHNHIIFSSTNLKTGKAFRWQKGTKKVFEQISDKIAAKEGAKIIEKSPKNS
HKKYTMWETESLYKEKIKSRLDFLLEQSSSINDFLEKAEALNLSVDFSKKWTTYRLLDEPQIKNTRSRSL
SKSAPTRYNYEKIVERLKENKSVLTLKEVVEKYVEKSEKAKNEFDYQLTIDEWQISHKTTRGYYVNVDFG
FGERGKVFIGAYKVDPLENGQYNIYVKRKDFFYLMNEKNADRNRYMTGETLIKQLRLYNGQTPLKKEPVM
RTIDELVNAINFLAANEIEDTRQLELLEEKLEAAFLEAEETLETLDEKMLELHQLSNLLLENELNGELEL
IQQKLKTLLPDATLAEFSYEDVRGEIEAIKTSQSLLENKLERTRNEINQLHEIQAVQKKETANQNNVKPK
L
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Auxiliary protein
| ID | 6113 | GenBank | WP_002367696 |
| Name | WP_002367696_ES-1348|unnamed1 |
UniProt ID | Q82YL4 |
| Length | 118 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
Auxiliary protein sequence
Download Length: 118 a.a. Molecular weight: 14042.10 Da Isoelectric Point: 9.9724
>WP_002367696.1 MULTISPECIES: plasmid mobilization relaxosome protein MobC [Bacteria]
MENKQKLKRPIQRKIRFSKEENELINRKIEESFFPNFQNFALHLLIQGEIRHVDYSELNRLTTEIHKIGI
NINQMARLANQFHEISSEDIKDLTDKVQSLNALVQSELNKLIKRKEQS
MENKQKLKRPIQRKIRFSKEENELINRKIEESFFPNFQNFALHLLIQGEIRHVDYSELNRLTTEIHKIGI
NINQMARLANQFHEISSEDIKDLTDKVQSLNALVQSELNKLIKRKEQS
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | Q82YL4 |
T4CP
| ID | 12141 | GenBank | WP_077932944 |
| Name | t4cp2_RX144_RS14440_ES-1348|unnamed1 |
UniProt ID | _ |
| Length | 609 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 609 a.a. Molecular weight: 70131.11 Da Isoelectric Point: 6.1811
>WP_077932944.1 VirD4-like conjugal transfer protein, CD1115 family [Enterococcus faecalis]
MQTYKKELKPYLYLGVFLFVIGFGIGNFFWLLPGTDYFSKTNYLFTKDILTYIEQTFYRFFITPSLLGFS
VGILGFLLGLLMYVRDNDRGIYRHGEEYGSARFATPAEMKKYEDPIPENNIIVSKHVKISLFNKRLPIKL
QKNKNIAILGDSGAAKTLAFIKTNLMQRHASFITTDPDGGILPEIGLLLKKGKYKIKVLDLNTLSNSDTF
NVFEYMHSELDVDRVLEAVTEATKEGEKTTGEDFWIKAEGLLVRSLIAYLWFDGRRNDYTPHLGMIADML
RHLKRKDKNVPSPVEEWFEELNEAIPDNYAYRQWTLFNNLYESETRASVLGIAAARYSVFDHEEVVNMIR
TDTMDIDSWNEEKTAVFIAIPETNTSFNFIAALMFATVGEVLRNKSDQVRLGKRVLEEGKELLHVRFLID
EFANIGRIPHFEKMLTTFRKREMSFTIVLQSLNQLQTLYRNGWQNILNGCACLLYLGGDEEETTKYLSAR
AGKQTLSIRKHSMNKGNQGGGSENRDKYGRDLIDRSEVALIGGDECLVFISKEHVFKDKKFYAFQHDRAD
ELATGPTDDKWYTWRRYMTDEEAILDKISATDIIDHGEITSELKEETHV
MQTYKKELKPYLYLGVFLFVIGFGIGNFFWLLPGTDYFSKTNYLFTKDILTYIEQTFYRFFITPSLLGFS
VGILGFLLGLLMYVRDNDRGIYRHGEEYGSARFATPAEMKKYEDPIPENNIIVSKHVKISLFNKRLPIKL
QKNKNIAILGDSGAAKTLAFIKTNLMQRHASFITTDPDGGILPEIGLLLKKGKYKIKVLDLNTLSNSDTF
NVFEYMHSELDVDRVLEAVTEATKEGEKTTGEDFWIKAEGLLVRSLIAYLWFDGRRNDYTPHLGMIADML
RHLKRKDKNVPSPVEEWFEELNEAIPDNYAYRQWTLFNNLYESETRASVLGIAAARYSVFDHEEVVNMIR
TDTMDIDSWNEEKTAVFIAIPETNTSFNFIAALMFATVGEVLRNKSDQVRLGKRVLEEGKELLHVRFLID
EFANIGRIPHFEKMLTTFRKREMSFTIVLQSLNQLQTLYRNGWQNILNGCACLLYLGGDEEETTKYLSAR
AGKQTLSIRKHSMNKGNQGGGSENRDKYGRDLIDRSEVALIGGDECLVFISKEHVFKDKKFYAFQHDRAD
ELATGPTDDKWYTWRRYMTDEEAILDKISATDIIDHGEITSELKEETHV
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 26667..41044
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| RX144_RS14420 (RX144_14420) | 21864..23549 | - | 1686 | WP_316498399 | relaxase/mobilization nuclease domain-containing protein | - |
| RX144_RS14425 (RX144_14425) | 23550..23906 | - | 357 | WP_002367696 | plasmid mobilization relaxosome protein MobC | - |
| RX144_RS14430 (RX144_14430) | 24152..24424 | - | 273 | WP_316498381 | LtrD | - |
| RX144_RS14435 (RX144_14435) | 24457..26616 | - | 2160 | WP_316498383 | ArdC-like ssDNA-binding domain-containing protein | - |
| RX144_RS14440 (RX144_14440) | 26667..28496 | - | 1830 | WP_077932944 | VirD4-like conjugal transfer protein, CD1115 family | virb4 |
| RX144_RS14445 (RX144_14445) | 28496..28981 | - | 486 | WP_316498384 | DUF3801 domain-containing protein | gbs1365 |
| RX144_RS14450 (RX144_14450) | 29023..29205 | - | 183 | WP_002367689 | hypothetical protein | - |
| RX144_RS14455 (RX144_14455) | 29251..29709 | - | 459 | WP_002370354 | single-stranded DNA-binding protein | - |
| RX144_RS14460 (RX144_14460) | 29726..30481 | - | 756 | WP_002367687 | Fic family protein | - |
| RX144_RS14465 (RX144_14465) | 30594..31202 | - | 609 | WP_316498385 | PcfA protein | - |
| RX144_RS14470 (RX144_14470) | 31186..31431 | - | 246 | WP_002393743 | hypothetical protein | - |
| RX144_RS14475 (RX144_14475) | 31418..32044 | - | 627 | WP_010784490 | hypothetical protein | prgL |
| RX144_RS14480 (RX144_14480) | 32068..34683 | - | 2616 | WP_202241304 | phage tail tip lysozyme | prgK |
| RX144_RS14485 (RX144_14485) | 34695..37085 | - | 2391 | WP_316498388 | VirB4-like conjugal transfer ATPase, CD1110 family | virb4 |
| RX144_RS14490 (RX144_14490) | 37039..37392 | - | 354 | WP_002367919 | PrgI family mobile element protein | prgIc |
| RX144_RS14495 (RX144_14495) | 37394..38194 | - | 801 | WP_002365861 | PrgH | prgHb |
| RX144_RS14500 (RX144_14500) | 38209..38505 | - | 297 | WP_002387740 | hypothetical protein | - |
| RX144_RS14505 (RX144_14505) | 38518..38745 | - | 228 | WP_002365858 | hypothetical protein | - |
| RX144_RS14510 (RX144_14510) | 38792..39226 | - | 435 | WP_316498389 | type III secretion system protein PrgE | - |
| RX144_RS14515 (RX144_14515) | 39246..40145 | - | 900 | WP_316498390 | type III secretion system protein PrgD | - |
| RX144_RS14520 (RX144_14520) | 40187..41044 | - | 858 | WP_316498391 | pheromone response LPXTG-anchored adhesin PrgC | prgC |
| RX144_RS14525 (RX144_14525) | 41072..41428 | - | 357 | WP_002367672 | pheromone response system RNA-binding regulator PrgU | - |
Host bacterium
| ID | 16697 | GenBank | NZ_CP136352 |
| Plasmid name | ES-1348|unnamed1 | Incompatibility group | - |
| Plasmid size | 44886 bp | Coordinate of oriT [Strand] | 23973..24022 [-] |
| Host baterium | Enterococcus faecalis strain ES-1348 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | prgB/asc10 |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIA9 |