Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 116251 |
| Name | oriT_IPPBiotE33|unnamed1  |
| Organism | Enterobacter hormaechei subsp. xiangfangensis strain IPPBiotE33 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP114034 (35698..35803 [+], 106 nt) |
| oriT length | 106 nt |
| IRs (inverted repeats) | 32..37, 42..47 (GCCGAC..GTCGGC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 106 nt
>oriT_IPPBiotE33|unnamed1
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGTCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGTCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 10394 | GenBank | WP_159190230 |
| Name | traI_O0R42_RS22180_IPPBiotE33|unnamed1 |
UniProt ID | _ |
| Length | 659 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75206.92 Da Isoelectric Point: 9.9948
>WP_159190230.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSENDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSENDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 12132 | GenBank | WP_004187443 |
| Name | t4cp2_O0R42_RS21915_IPPBiotE33|unnamed1 |
UniProt ID | _ |
| Length | 695 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 695 a.a. Molecular weight: 79519.87 Da Isoelectric Point: 4.8309
>WP_004187443.1 MULTISPECIES: hypothetical protein [Enterobacterales]
MQQESVDSKKVIRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMHFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
MQQESVDSKKVIRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMHFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 38599..57882
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| O0R42_RS22160 (O0R42_22135) | 33684..33995 | + | 312 | WP_053919080 | hypothetical protein | - |
| O0R42_RS22165 (O0R42_22140) | 34128..34664 | + | 537 | WP_011091066 | hypothetical protein | - |
| O0R42_RS22170 (O0R42_22145) | 35165..35560 | - | 396 | WP_170949733 | MobC | - |
| O0R42_RS22175 (O0R42_22150) | 35595..36122 | + | 528 | WP_316350754 | plasmid mobilization protein MobA | - |
| O0R42_RS22180 (O0R42_22155) | 36109..38088 | + | 1980 | WP_159190230 | TraI/MobA(P) family conjugative relaxase | - |
| O0R42_RS22185 (O0R42_22160) | 38102..38602 | + | 501 | WP_004187320 | DotD/TraH family lipoprotein | - |
| O0R42_RS22190 (O0R42_22165) | 38599..39378 | + | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| O0R42_RS22195 (O0R42_22170) | 39389..40552 | + | 1164 | WP_040027864 | plasmid transfer ATPase TraJ | virB11 |
| O0R42_RS22200 (O0R42_22175) | 40542..40802 | + | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| O0R42_RS22205 (O0R42_22180) | 40827..44219 | + | 3393 | WP_316350755 | LPD7 domain-containing protein | - |
| O0R42_RS22210 (O0R42_22185) | 44185..44697 | + | 513 | WP_011091071 | hypothetical protein | traL |
| O0R42_RS22215 (O0R42_22190) | 44698..44910 | - | 213 | WP_305953721 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| O0R42_RS22220 (O0R42_22195) | 44882..45292 | - | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| O0R42_RS22225 (O0R42_22200) | 45360..46073 | + | 714 | WP_004187467 | DotI/IcmL family type IV secretion protein | traM |
| O0R42_RS22230 (O0R42_22205) | 46082..47233 | + | 1152 | WP_004187471 | DotH/IcmK family type IV secretion protein | traN |
| O0R42_RS22235 (O0R42_22210) | 47245..48594 | + | 1350 | WP_004187474 | conjugal transfer protein TraO | traO |
| O0R42_RS22240 (O0R42_22215) | 48606..49310 | + | 705 | WP_015060002 | conjugal transfer protein TraP | traP |
| O0R42_RS22245 (O0R42_22220) | 49334..49864 | + | 531 | WP_004187478 | conjugal transfer protein TraQ | traQ |
| O0R42_RS22250 (O0R42_22225) | 49881..50270 | + | 390 | WP_011154472 | DUF6750 family protein | traR |
| O0R42_RS22255 (O0R42_22230) | 50316..50810 | + | 495 | WP_011154473 | hypothetical protein | - |
| O0R42_RS22260 (O0R42_22235) | 50807..53857 | + | 3051 | WP_011154474 | conjugative transfer protein | traU |
| O0R42_RS22265 (O0R42_22240) | 53854..55062 | + | 1209 | WP_316350756 | conjugal transfer protein TraW | traW |
| O0R42_RS22270 (O0R42_22245) | 55059..55709 | + | 651 | WP_011091083 | hypothetical protein | - |
| O0R42_RS22275 (O0R42_22250) | 55702..57882 | + | 2181 | WP_004187492 | DotA/TraY family protein | traY |
| O0R42_RS22280 (O0R42_22255) | 57885..58538 | + | 654 | WP_004187494 | hypothetical protein | - |
| O0R42_RS22285 (O0R42_22260) | 58613..58843 | + | 231 | WP_011091085 | IncL/M type plasmid replication protein RepC | - |
Host bacterium
| ID | 16684 | GenBank | NZ_CP114034 |
| Plasmid name | IPPBiotE33|unnamed1 | Incompatibility group | IncL/M |
| Plasmid size | 59140 bp | Coordinate of oriT [Strand] | 35698..35803 [+] |
| Host baterium | Enterobacter hormaechei subsp. xiangfangensis strain IPPBiotE33 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |