Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   116240
Name   oriT_pTQ28-tet(X4) in_silico
Organism   Morganella morganii strain TQ28
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_ON390816 (108324..108608 [-], 285 nt)
oriT length   285 nt
IRs (inverted repeats)      184..189, 191..196  (AAAAGT..ACTTTT)
Location of nic site      109..110
Conserved sequence flanking the
  nic site    		  TTTGGTTAAA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 285 nt

>oriT_pTQ28-tet(X4)
GGTTATTGCTACTTAATGCCGATAACGACTCAGGCTTTGAGGTTTTTTTATACGGTTCACATTTCGTTAGCAAGGTCAGGGTTTTTTGATAAAATTCTGGTTAGTTTGGTTAAAAAGTGTTACAAGTAAGGGTAATGGCTGAAAGGTTAGTTTTAAGGTTCAAAGCGGCAGTATTAAAATTCCAAAAGTTACTTTTCATCCTTCAGAATCCAGACCTTAATTTCATGTAGAAGATTCGTACAATTGTATTGGCGCAAGGACAATCCGCACATGTCAGAATCAGAT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   10388 GenBank   WP_039026431
Name   mobH_ODS32_RS00580_pTQ28-tet(X4) insolico UniProt ID   A0A8E6NZY6
Length   1011 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1011 a.a.        Molecular weight: 112997.99 Da        Isoelectric Point: 4.5108

>WP_039026431.1 MULTISPECIES: MobH family relaxase [Enterobacterales]
MNFRALFLSMQRVFGIFSRRENDVSELMMKDAANFSPFAQIIGEQKYTVPDHPNPEVLKFIEYPTRPAGI
QTFNEQSILSLYRDKLHSISMMLAISDGDIREDAYTFTNLVLKPLIEYIRWIHLLPASENHHHNGIGGLL
SHSLEVAMISLKNANHSELRPIGYQDEEVVRRKVYLYAAFICGLVHDAGKVYDLDIVSLNLSETLTWAPS
SQSLLDWARENNVVEYEIHWRKRIHNQHNIWSSVFLERILDPVCMSFLDRVKKERVYAKMVTALNVYNDG
NDFLSKCVRTSDYYSTGTDLNVLRDPIMGLRSNDAAARAIGTIKHNFTSININNYKTKPMHIIIVNGEVY
LNENAFLDFVLSDFAAHKFNFPQGDAGKTVLVESLVLRGYVEPYDDERVVHYFIPGTYSENEIASIFRNG
IGKLEFYNLLKLRWIGLIFDSYKIPDSVPGLFSVNANKDFIYIDEQKTVTEYRRPVPGRESVTRVTDTVN
DAIENTPQYGLQLVNGPDADSNNIISSENTESITDSLEESGADISNEIFETQVVTAIDTAETVNADEPEQ
VEEHDDRSQIHLVEQLHEMLLSAPLPHHAVINIDSVPYLDLDAAIALIPGIDEAAFCNGPFFQLTYRDGS
LDGMWIVRDVNNLRLIQLGDNCAGMQVSTSEPRNTSSLKSLFDTSMYQPLDIPEAPSVNEAASPPQTPLE
LPQPRLNAPVAEEASSVAEQTNAHSEPDSVIATEYEQYGHLLEETLDSDGEAYSDLIASDSTEAEYSATD
PQSSDFAQLPRETALSVAPGDLDYSEGAIKPPAPDATGKETILTSPEPAEDVRETVAAVEKASHLSPALA
RLFAVSTHAEKKHEKTQEPSPVKEVKNPTSSTTVKAPISIEPPGAEEKEAVEEFTLLNDGEVTELEYVEI
ATMLHQILTKLSGSFKRKRKNRFMVLTQNTFYLTQSCIEKYGTQLNAPELFNQLPQYQVTSGAVVNTKCI
AFNIPTLVAASDRAKVDIELIINKLKEVGNL

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(106-301)


  Protein structure



No available structure.




T4CP

ID   12127 GenBank   WP_000167418
Name   traD_ODS32_RS00585_pTQ28-tet(X4) insolico UniProt ID   _
Length   694 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 694 a.a.        Molecular weight: 77969.74 Da        Isoelectric Point: 8.7275

>WP_000167418.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Enterobacterales]
MTKSKRTNLHAQENFYRPILEYRSASVLLICAVIMLVMGFRSDGVNIAPIILYTAVFLLLVCLYRCKTAH
PYLMAHWRVFQRQIMFISLKSLRTINKSNFFSNERKYRQLVQEYKKNNRPVPDRKTYFCNGFEWGPEHAD
RAYQIANLSSDKREIALPFVLSPIARHFETMARSMGGNNAIFAVDRRAPIFVTEDNWFGHTLITGNVGTG
KTVLQRLLSISMLHLGHVIVVIDPKNDAEWRQSLMDEASELGLPFYKFHPAQPSSSVCIDVCNAYTNVSD
LTSRLLSLVSVPGEVNPFVQYAEALVSTVITGLSYTDKKPSIYLIHKNMKSHMSVVNLTIKVMECCFARH
YGPDVWMEKVKYASNDTLQVRFKRLTEWFNAHFLNYEGAEPIEWIDTVGRLVDYSMSDPEHMSKMTAGIM
PLFSRLTEQPLNELLSPSPNTLTSREIVTSDGMFSTGGVLYISLDGLSNPESARAISQLIMSDLTSCAGS
RYNADDGDMSSHSRISIFVDEAHSAINNSMINLLAQGRAAQIALFICTQTISDFIAAANAETANRITGLC
NNYISLRVNDTPTQTLVVENFGKSPISTNMVTYTTGSETTLPHNNFSGSISERKQTTLEESIPKELLGQV
PKFHIVARLQDGRKVVGQIPIAVSEKAMKPNTTLLEMFLKPAGKVTLRQNVGLSYLNKYLRKLH

  Protein domains


Predicted by InterproScan.

AAA-like domain (AAA_10)

(199-245)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(410-638)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 173447..182615

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ODS32_RS00950 168984..169184 - 201 WP_000357614 hypothetical protein -
ODS32_RS00955 169202..170278 - 1077 WP_001015069 hypothetical protein -
ODS32_RS00960 170813..171688 + 876 WP_001282731 RepB family plasmid replication initiator protein -
ODS32_RS00965 172135..172560 - 426 WP_154166887 hypothetical protein -
ODS32_RS00970 173042..173395 + 354 WP_000424120 pili assembly chaperone -
ODS32_RS00975 173447..173764 + 318 WP_000203871 type IV conjugative transfer system protein TraL traL
ODS32_RS00980 173776..174561 + 786 WP_000771920 TraE/TraK family type IV conjugative transfer system protein traE
ODS32_RS00985 174606..175796 + 1191 WP_225374337 type-F conjugative transfer system secretin TraK traK
ODS32_RS00990 175798..176238 + 441 WP_001224094 plasmid transfer protein HtdO -
ODS32_RS00995 176228..177586 + 1359 WP_000351840 IncHI-type conjugal transfer protein TrhB traB
ODS32_RS01000 177595..178107 + 513 WP_000429933 hypothetical protein -
ODS32_RS01005 178213..178965 + 753 WP_010892304 protein-disulfide isomerase HtdT -
ODS32_RS01010 178975..179925 + 951 WP_001022266 TraV family lipoprotein traV
ODS32_RS01015 179934..182615 + 2682 WP_001255540 IncHI-type conjugal transfer ATPase TrhC virb4

Host bacterium

ID   16673 GenBank   NZ_ON390816
Plasmid name   pTQ28-tet(X4) Incompatibility group   IncFIA
Plasmid size   187191 bp Coordinate of oriT [Strand]   108324..108608 [-]
Host baterium   Morganella morganii strain TQ28

Cargo genes

Drug resistance gene   tet(X4), floR, qnrS1, blaTEM-1B, ant(3'')-Ia, lnu(G)
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -