Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   116236
Name   oriT_pXY36-tet(X4) in_silico
Organism   Morganella morganii strain XY36
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_ON390820 (114977..115261 [-], 285 nt)
oriT length   285 nt
IRs (inverted repeats)      184..189, 191..196  (AAAAGT..ACTTTT)
Location of nic site      109..110
Conserved sequence flanking the
  nic site    		  TTTGGTTAAA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 285 nt

>oriT_pXY36-tet(X4)
GGTTATTGCTACTTAATGCCGATAACGACTCAGGCTTTGAGGTTTTTTTATACGGTTCACATTTCGTTAGCAAGGTCAGGGTTTTTTGATAAAATTCTGGTTAGTTTGGTTAAAAAGTGTTACAAGTAAGGGTAATGGCTGAAAGGTTAGTTTTAAGGTTCAAAGCGGCAGTATTAAAATTCCAAAAGTTACTTTTCATCCTTCAGAATCCAGACCTTAATTTCATGTAGAAGATTCGTACAATTGTATTGGCGCAAGGACAATCCGCACATGTCAGAATCAGAT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   10386 GenBank   WP_039026431
Name   mobH_ODS34_RS00610_pXY36-tet(X4) insolico UniProt ID   A0A8E6NZY6
Length   1011 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1011 a.a.        Molecular weight: 112997.99 Da        Isoelectric Point: 4.5108

>WP_039026431.1 MULTISPECIES: MobH family relaxase [Enterobacterales]
MNFRALFLSMQRVFGIFSRRENDVSELMMKDAANFSPFAQIIGEQKYTVPDHPNPEVLKFIEYPTRPAGI
QTFNEQSILSLYRDKLHSISMMLAISDGDIREDAYTFTNLVLKPLIEYIRWIHLLPASENHHHNGIGGLL
SHSLEVAMISLKNANHSELRPIGYQDEEVVRRKVYLYAAFICGLVHDAGKVYDLDIVSLNLSETLTWAPS
SQSLLDWARENNVVEYEIHWRKRIHNQHNIWSSVFLERILDPVCMSFLDRVKKERVYAKMVTALNVYNDG
NDFLSKCVRTSDYYSTGTDLNVLRDPIMGLRSNDAAARAIGTIKHNFTSININNYKTKPMHIIIVNGEVY
LNENAFLDFVLSDFAAHKFNFPQGDAGKTVLVESLVLRGYVEPYDDERVVHYFIPGTYSENEIASIFRNG
IGKLEFYNLLKLRWIGLIFDSYKIPDSVPGLFSVNANKDFIYIDEQKTVTEYRRPVPGRESVTRVTDTVN
DAIENTPQYGLQLVNGPDADSNNIISSENTESITDSLEESGADISNEIFETQVVTAIDTAETVNADEPEQ
VEEHDDRSQIHLVEQLHEMLLSAPLPHHAVINIDSVPYLDLDAAIALIPGIDEAAFCNGPFFQLTYRDGS
LDGMWIVRDVNNLRLIQLGDNCAGMQVSTSEPRNTSSLKSLFDTSMYQPLDIPEAPSVNEAASPPQTPLE
LPQPRLNAPVAEEASSVAEQTNAHSEPDSVIATEYEQYGHLLEETLDSDGEAYSDLIASDSTEAEYSATD
PQSSDFAQLPRETALSVAPGDLDYSEGAIKPPAPDATGKETILTSPEPAEDVRETVAAVEKASHLSPALA
RLFAVSTHAEKKHEKTQEPSPVKEVKNPTSSTTVKAPISIEPPGAEEKEAVEEFTLLNDGEVTELEYVEI
ATMLHQILTKLSGSFKRKRKNRFMVLTQNTFYLTQSCIEKYGTQLNAPELFNQLPQYQVTSGAVVNTKCI
AFNIPTLVAASDRAKVDIELIINKLKEVGNL

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(106-301)


  Protein structure



No available structure.




T4CP

ID   12125 GenBank   WP_000167418
Name   traD_ODS34_RS00615_pXY36-tet(X4) insolico UniProt ID   _
Length   694 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 694 a.a.        Molecular weight: 77969.74 Da        Isoelectric Point: 8.7275

>WP_000167418.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Enterobacterales]
MTKSKRTNLHAQENFYRPILEYRSASVLLICAVIMLVMGFRSDGVNIAPIILYTAVFLLLVCLYRCKTAH
PYLMAHWRVFQRQIMFISLKSLRTINKSNFFSNERKYRQLVQEYKKNNRPVPDRKTYFCNGFEWGPEHAD
RAYQIANLSSDKREIALPFVLSPIARHFETMARSMGGNNAIFAVDRRAPIFVTEDNWFGHTLITGNVGTG
KTVLQRLLSISMLHLGHVIVVIDPKNDAEWRQSLMDEASELGLPFYKFHPAQPSSSVCIDVCNAYTNVSD
LTSRLLSLVSVPGEVNPFVQYAEALVSTVITGLSYTDKKPSIYLIHKNMKSHMSVVNLTIKVMECCFARH
YGPDVWMEKVKYASNDTLQVRFKRLTEWFNAHFLNYEGAEPIEWIDTVGRLVDYSMSDPEHMSKMTAGIM
PLFSRLTEQPLNELLSPSPNTLTSREIVTSDGMFSTGGVLYISLDGLSNPESARAISQLIMSDLTSCAGS
RYNADDGDMSSHSRISIFVDEAHSAINNSMINLLAQGRAAQIALFICTQTISDFIAAANAETANRITGLC
NNYISLRVNDTPTQTLVVENFGKSPISTNMVTYTTGSETTLPHNNFSGSISERKQTTLEESIPKELLGQV
PKFHIVARLQDGRKVVGQIPIAVSEKAMKPNTTLLEMFLKPAGKVTLRQNVGLSYLNKYLRKLH

  Protein domains


Predicted by InterproScan.

AAA-like domain (AAA_10)

(199-245)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(410-638)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 178901..188069

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ODS34_RS00975 174438..174638 - 201 WP_000357614 hypothetical protein -
ODS34_RS00980 174656..175732 - 1077 WP_001015069 hypothetical protein -
ODS34_RS00985 176267..177142 + 876 WP_001282731 RepB family plasmid replication initiator protein -
ODS34_RS00990 177589..178014 - 426 WP_154166887 hypothetical protein -
ODS34_RS00995 178496..178849 + 354 WP_000424120 pili assembly chaperone -
ODS34_RS01000 178901..179218 + 318 WP_000203871 type IV conjugative transfer system protein TraL traL
ODS34_RS01005 179218..180015 + 798 WP_011011069 TraE/TraK family type IV conjugative transfer system protein traE
ODS34_RS01010 180060..181250 + 1191 WP_225374337 type-F conjugative transfer system secretin TraK traK
ODS34_RS01015 181252..181692 + 441 WP_001224094 plasmid transfer protein HtdO -
ODS34_RS01020 181682..183040 + 1359 WP_000351840 IncHI-type conjugal transfer protein TrhB traB
ODS34_RS01025 183049..183561 + 513 WP_000429933 hypothetical protein -
ODS34_RS01030 183667..184419 + 753 WP_010892304 protein-disulfide isomerase HtdT -
ODS34_RS01035 184429..185379 + 951 WP_001022266 TraV family lipoprotein traV
ODS34_RS01040 185388..188069 + 2682 WP_001255540 IncHI-type conjugal transfer ATPase TrhC virb4
ODS34_RS01045 188736..189164 + 429 WP_124061973 hypothetical protein -

Host bacterium

ID   16669 GenBank   NZ_ON390820
Plasmid name   pXY36-tet(X4) Incompatibility group   IncFIA
Plasmid size   190661 bp Coordinate of oriT [Strand]   114977..115261 [-]
Host baterium   Morganella morganii strain XY36

Cargo genes

Drug resistance gene   tet(X4), floR, qnrS1, blaTEM-1B, ant(3'')-Ia, lnu(G)
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -