Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   116232
Name   oriT_pSF3 in_silico
Organism   Enterococcus faecalis strain SF28073
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP060803 (39387..39416 [+], 30 nt)
oriT length   30 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 30 nt

>oriT_pSF3
AACGTAAGTTAGTGGGTATAAGTGCGCCCT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   10384 GenBank   WP_000974115
Name   mobQ_H9Q64_RS01025_pSF3 insolico UniProt ID   A0A7H0FKC2
Length   661 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 661 a.a.        Molecular weight: 77412.46 Da        Isoelectric Point: 9.3565

>WP_000974115.1 MULTISPECIES: MobQ family relaxase [Bacilli]
MAIFHLSMTIAKREGGKRSLIAMASYRSGEKLYSELYEKTNLYNHRTVKPEAFILKPDYVPNEFLDRQTL
WNKMELAEKSPNAQLCREVNVALPIELNNSDQRMLIEDFVKDNFVSEGMIADVAIHRDDENNPHAHIMLT
MREVDSEGNILNKRKRIPKLDENGNQIFNEKGQRVTVSIKTNDWDRKSLVSEIRKDWADKVNQYLKDRNI
DQQITEKSHAELGKKELPTIHEGFYSKKLEDKGVISELKRKNLEIQSYNDVLSELDKLENQEKVLKQDQN
FTLKFEKTFSPLEKKELKNLSKELKIFVNDENIDKRISELKRWENSLIFNNKMEIQKQRLMLSKISSERD
MLTKANEILDKQAERFFKKSYPSLNIDKFSNHEVRAMVNETIFRKQLLNKDQLAEVIYNERVVEKEESKK
IFKEKPFQTSRYLDSKIKQVEDSITKENNPERKEILSIKKEKLIGIKQGLIEYVQSEVERKFDKNVSIDS
VIEGEMLLAKADYYKTTDFSKVEGVARFSSEEINSMLEQSKGFLTNIQTVKIPNDCQGVFFVQDSMKHID
ELSPLAKHNLKKVVNRNAYLPDSDKIELSKEIENTNKDQSQELDKDVPEKNEVTVKMFQFAKSINRLLSG
NQLQKKRNLDKLIKQTKAKENQSLQRNIPLR

  Protein domains


Predicted by InterproScan.

MobA/MobL family (MobA_MobL)

(18-242)

Nicking enzyme C-terminal middle helical domain (NES_C_h)

(289-394)


  Protein structure


Source ID Structure
AlphaFold DB A0A7H0FKC2


T4CP

ID   12122 GenBank   WP_000096577
Name   t4cp2_H9Q64_RS00980_pSF3 insolico UniProt ID   _
Length   551 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 551 a.a.        Molecular weight: 63042.12 Da        Isoelectric Point: 4.7709

>WP_000096577.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Bacilli]
MSSLLAESDGLILGKFQSGNVVIQPEDSKISNRNIFVVGGPGSFKTQSYVLPNVVNNRSTSIVVTDPKGE
IYELTNEIKKAQGFKTVVINFKDFLLSSRYNPLLYIRKSNDTNKIANVIVSAKNDPKRKDFWFNAQLSLL
NTLIKYVYFEYEPSARTIEGILDFLEEFDPRYNEEGVSELDEQFERLPDGHEAKRSYYLGFRQAQSEARP
NIVISLLTTLQDFVDKEVSEFTSTNDFFFEELGTSKICLYVLISPLDRTWDGLVNLFFQQMFTELYFLGD
KHNAKLPVPLVMLLDEFVNLGYFPTYENFLATCRGYRISVSTILQSLPQGFELYGDKKFKAIIGNHAIKI
CLGGVEETTAEYFSRQVNDTTIKVYTGGTSESKTSAKTTNRSGSKSESYGYQKRRLITEGEVINLQQEEN
GRKSIVLIDGKPYMLRKTPQFELFGNLLKKHEISQQDYISSQTEFAKEYIEELEKQHKQRKVQLASAPIF
HEKKQEDDLNKKEINLPEQPIESEVKEEAEEKELLMSDILSSFDEGIEENTANNDNSELPF

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(33-456)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 24745..36921

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9Q64_RS00925 (H9Q64_00925) 20231..20593 - 363 WP_000108744 toprim domain-containing protein -
H9Q64_RS00930 (H9Q64_00930) 20593..21210 - 618 WP_001062588 recombinase family protein -
H9Q64_RS00935 (H9Q64_00935) 21224..21394 - 171 WP_000713595 hypothetical protein -
H9Q64_RS00940 (H9Q64_00940) 21742..23232 - 1491 WP_001025005 primase C-terminal domain-containing protein -
H9Q64_RS00945 (H9Q64_00945) 23636..23884 - 249 WP_000362415 helix-turn-helix domain-containing protein -
H9Q64_RS00950 (H9Q64_00950) 24322..24426 + 105 WP_113792438 type I toxin-antitoxin system Fst family toxin -
H9Q64_RS00955 (H9Q64_00955) 24745..25458 - 714 WP_000717317 LPXTG cell wall anchor domain-containing protein prgC
H9Q64_RS00960 (H9Q64_00960) 25518..25886 - 369 WP_000516429 hypothetical protein -
H9Q64_RS00965 (H9Q64_00965) 25915..26883 - 969 WP_000122915 hypothetical protein -
H9Q64_RS00970 (H9Q64_00970) 26900..27832 - 933 WP_000382494 conjugal transfer protein TrbL family protein trsL
H9Q64_RS00975 (H9Q64_00975) 27834..28757 - 924 WP_001013727 hypothetical protein traP
H9Q64_RS00980 (H9Q64_00980) 28775..30430 - 1656 WP_000096577 VirD4-like conjugal transfer protein, CD1115 family -
H9Q64_RS00985 (H9Q64_00985) 30423..30854 - 432 WP_001085135 hypothetical protein -
H9Q64_RS00990 (H9Q64_00990) 30859..31410 - 552 WP_000393725 hypothetical protein -
H9Q64_RS00995 (H9Q64_00995) 31423..32532 - 1110 WP_000501303 lysozyme family protein orf14
H9Q64_RS01000 (H9Q64_01000) 32554..33906 - 1353 WP_000875579 hypothetical protein trsF
H9Q64_RS01005 (H9Q64_01005) 33920..35881 - 1962 WP_000411529 VirB4 family type IV secretion system protein virb4
H9Q64_RS01010 (H9Q64_01010) 35892..36521 - 630 WP_000459258 hypothetical protein trsD
H9Q64_RS01015 (H9Q64_01015) 36538..36921 - 384 WP_001083514 glycosyl transferase trsC
H9Q64_RS01020 (H9Q64_01020) 36940..37272 - 333 WP_000713503 CagC family type IV secretion system protein -
H9Q64_RS01025 (H9Q64_01025) 37296..39281 - 1986 WP_000974115 MobQ family relaxase -
H9Q64_RS01030 (H9Q64_01030) 39573..39872 + 300 WP_000431202 hypothetical protein -
H9Q64_RS01035 (H9Q64_01035) 39875..40132 + 258 WP_000002668 hypothetical protein -
H9Q64_RS01040 (H9Q64_01040) 40155..40460 - 306 WP_000351790 hypothetical protein -
H9Q64_RS01045 (H9Q64_01045) 40494..40991 - 498 WP_000874092 DnaJ domain-containing protein -
H9Q64_RS01050 (H9Q64_01050) 41030..41347 - 318 WP_000415964 hypothetical protein -

Host bacterium

ID   16665 GenBank   NZ_CP060803
Plasmid name   pSF3 Incompatibility group   -
Plasmid size   46541 bp Coordinate of oriT [Strand]   39387..39416 [+]
Host baterium   Enterococcus faecalis strain SF28073

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -