Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 116183 |
| Name | oriT_pECD227_112  |
| Organism | Escherichia fergusonii ECD227 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CM001143 (58049..58135 [-], 87 nt) |
| oriT length | 87 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 87 nt
>oriT_pECD227_112
GGGGTGTCGGGGCGAAGCCCTGACCAGGAGGCAATTGTATAATCGCGCGTGCGCGGTTATACAATTGCACATCCTGTCCCGTTTTTC
GGGGTGTCGGGGCGAAGCCCTGACCAGGAGGCAATTGTATAATCGCGCGTGCGCGGTTATACAATTGCACATCCTGTCCCGTTTTTC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 12094 | GenBank | WP_001289276 |
| Name | trbC_ECD227_RS00935_pECD227_112 |
UniProt ID | _ |
| Length | 763 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 763 a.a. Molecular weight: 86941.11 Da Isoelectric Point: 6.7713
>WP_001289276.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELH
PGEFFSIFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHPTGRPVNGFHHKKTNRPDWDGMY
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELH
PGEFFSIFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHPTGRPVNGFHHKKTNRPDWDGMY
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 60870..98004
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| ECD227_RS27285 | 56103..56300 | - | 198 | WP_001161115 | hypothetical protein | - |
| ECD227_RS00945 (ECD227_4148) | 56649..57497 | + | 849 | WP_001077023 | DUF4942 domain-containing protein | - |
| ECD227_RS00940 (ECD227_4149) | 57583..57918 | - | 336 | WP_000157095 | molybdopterin-guanine dinucleotide biosynthesis protein MobC | - |
| ECD227_RS23295 | 58151..58412 | + | 262 | WP_001281050 | plasmid mobilization protein MobA | - |
| ECD227_RS00935 (ECD227_4150) | 58586..60877 | - | 2292 | WP_001289276 | F-type conjugative transfer protein TrbC | - |
| ECD227_RS00930 (ECD227_4151) | 60870..61940 | - | 1071 | WP_000151583 | IncI1-type conjugal transfer protein TrbB | trbB |
| ECD227_RS00925 (ECD227_4152) | 61959..63167 | - | 1209 | WP_000121274 | IncI1-type conjugal transfer protein TrbA | trbA |
| ECD227_RS23300 | 63459..63611 | + | 153 | WP_001331364 | Hok/Gef family protein | - |
| ECD227_RS27290 | 63683..63874 | - | 192 | WP_174715448 | hypothetical protein | - |
| ECD227_RS27295 | 64434..64529 | + | 96 | WP_000609148 | DinQ-like type I toxin DqlB | - |
| ECD227_RS26760 | 64594..64770 | - | 177 | WP_001054904 | hypothetical protein | - |
| ECD227_RS23315 | 65162..65371 | + | 210 | WP_000062603 | HEAT repeat domain-containing protein | - |
| ECD227_RS00915 (ECD227_4154) | 65443..66105 | - | 663 | WP_000653334 | plasmid IncI1-type surface exclusion protein ExcA | - |
| ECD227_RS00910 (ECD227_4155) | 66170..68332 | - | 2163 | WP_000698351 | DotA/TraY family protein | traY |
| ECD227_RS00905 (ECD227_4156) | 68429..69013 | - | 585 | WP_001037987 | IncI1-type conjugal transfer protein TraX | - |
| ECD227_RS00900 (ECD227_4157) | 69042..70244 | - | 1203 | WP_001189160 | IncI1-type conjugal transfer protein TraW | traW |
| ECD227_RS23320 (ECD227_4158) | 70211..70825 | - | 615 | WP_000337398 | IncI1-type conjugal transfer protein TraV | traV |
| ECD227_RS00895 (ECD227_4159) | 70825..73869 | - | 3045 | WP_001024780 | IncI1-type conjugal transfer protein TraU | traU |
| ECD227_RS00890 (ECD227_4160) | 73959..74759 | - | 801 | WP_001164788 | IncI1-type conjugal transfer protein TraT | traT |
| ECD227_RS23325 | 74743..74931 | - | 189 | WP_001277255 | putative conjugal transfer protein TraS | - |
| ECD227_RS00885 (ECD227_4161) | 74995..75399 | - | 405 | WP_000086957 | IncI1-type conjugal transfer protein TraR | traR |
| ECD227_RS00880 (ECD227_4162) | 75450..75977 | - | 528 | WP_001055569 | conjugal transfer protein TraQ | traQ |
| ECD227_RS00875 (ECD227_4163) | 75977..76681 | - | 705 | WP_000801920 | IncI1-type conjugal transfer protein TraP | traP |
| ECD227_RS00870 (ECD227_4164) | 76681..77970 | - | 1290 | WP_001272005 | conjugal transfer protein TraO | traO |
| ECD227_RS00865 (ECD227_4165) | 77973..78956 | - | 984 | WP_001191877 | IncI1-type conjugal transfer protein TraN | traN |
| ECD227_RS00860 (ECD227_4166) | 78967..79659 | - | 693 | WP_000138548 | DotI/IcmL family type IV secretion protein | traM |
| ECD227_RS00855 (ECD227_4167) | 79656..80003 | - | 348 | WP_001055900 | conjugal transfer protein | traL |
| ECD227_RS00850 (ECD227_4168) | 80021..83788 | - | 3768 | WP_001141528 | LPD7 domain-containing protein | - |
| ECD227_RS23330 (ECD227_4169) | 83878..84429 | - | 552 | WP_000014583 | phospholipase D family protein | - |
| ECD227_RS25265 | 84444..84734 | - | 291 | WP_001299214 | hypothetical protein | traK |
| ECD227_RS00845 (ECD227_4170) | 84731..85879 | - | 1149 | WP_001024972 | plasmid transfer ATPase TraJ | virB11 |
| ECD227_RS23335 | 85876..86694 | - | 819 | WP_000646098 | IncI1-type conjugal transfer lipoprotein TraI | traI |
| ECD227_RS23340 | 86691..87149 | - | 459 | WP_001079808 | IncI1-type conjugal transfer lipoprotein TraH | - |
| ECD227_RS00840 (ECD227_4171) | 87544..88128 | - | 585 | WP_000977522 | histidine phosphatase family protein | - |
| ECD227_RS00835 (ECD227_4172) | 88188..89390 | - | 1203 | WP_000976351 | conjugal transfer protein TraF | - |
| ECD227_RS00830 (ECD227_4173) | 89475..90299 | - | 825 | WP_001238939 | conjugal transfer protein TraE | traE |
| ECD227_RS00825 (ECD227_4174) | 90450..91604 | - | 1155 | WP_001139957 | tyrosine-type recombinase/integrase | - |
| ECD227_RS27125 | 91771..91977 | + | 207 | WP_000326764 | hypothetical protein | - |
| ECD227_RS25275 | 91974..92312 | - | 339 | WP_072109855 | hypothetical protein | - |
| ECD227_RS23345 | 92413..93498 | - | 1086 | WP_001498285 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| ECD227_RS23350 (ECD227_4186) | 93498..94154 | - | 657 | WP_001193553 | prepilin peptidase | - |
| ECD227_RS23355 | 94139..94699 | - | 561 | WP_000014116 | lytic transglycosylase domain-containing protein | virB1 |
| ECD227_RS23360 (ECD227_4187) | 94709..95323 | - | 615 | WP_000959785 | type 4 pilus major pilin | - |
| ECD227_RS23365 | 95341..96438 | - | 1098 | WP_001208805 | type II secretion system F family protein | - |
| ECD227_RS00820 (ECD227_4188) | 96451..98004 | - | 1554 | WP_000362202 | GspE/PulE family protein | virB11 |
| ECD227_RS00815 (ECD227_4189) | 98015..98467 | - | 453 | WP_001247336 | type IV pilus biogenesis protein PilP | - |
| ECD227_RS23370 | 98454..99749 | - | 1296 | WP_000752774 | type 4b pilus protein PilO2 | - |
| ECD227_RS23375 | 99742..101424 | - | 1683 | WP_000748143 | PilN family type IVB pilus formation outer membrane protein | - |
| ECD227_RS00810 (ECD227_4190) | 101438..101875 | - | 438 | WP_000539807 | type IV pilus biogenesis protein PilM | - |
| ECD227_RS00805 (ECD227_4191) | 101875..102942 | - | 1068 | WP_000742600 | type IV pilus biogenesis lipoprotein PilL | - |
Host bacterium
| ID | 16616 | GenBank | NZ_CM001143 |
| Plasmid name | pECD227_112 | Incompatibility group | IncI1 |
| Plasmid size | 112394 bp | Coordinate of oriT [Strand] | 58049..58135 [-] |
| Host baterium | Escherichia fergusonii ECD227 |
Cargo genes
| Drug resistance gene | tet(A), sul1, qacE, aac(3)-VIa, ant(3'')-Ia |
| Virulence gene | htpB |
| Metal resistance gene | merR, merT, merP, merA, merD, merE |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |