Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   116159
Name   oriT_15-307-1|p15-307-1_03 in_silico
Organism   Enterococcus faecium strain 15-307-1
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP044319 (8929..8966 [+], 38 nt)
oriT length   38 nt
IRs (inverted repeats)      14..22, 29..37  (TAAAGTATA..TATACTTTA)
 2..8, 13..19  (ACTTTAT..ATAAAGT)
Location of nic site      27..28
Conserved sequence flanking the
  nic site  
 
 GTGTGTTATA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 38 nt

>oriT_15-307-1|p15-307-1_03
CACTTTATGAATATAAAGTATAGTGTGTTATACTTTAC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase


ID   10334 GenBank   WP_000119405
Name   mobV_CWC51_RS15665_15-307-1|p15-307-1_03 insolico UniProt ID   F5X340
Length   420 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 420 a.a.        Molecular weight: 49660.33 Da        Isoelectric Point: 9.9693

>WP_000119405.1 MULTISPECIES: MobV family relaxase [Bacteria]
MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENYDLKNDKNIDYNERVKEIIESQKTGTR
KTRKDAVLVNELLVTSDRDFFEQLDPGEQKRFFEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPM
RDGKLQGKNVFNRQELLWLQDKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVK
KDEWTAYSDKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTAARDN
DRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEENKSLKSKISDLKRDVSL
IYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHDLEPKKNEFELTHNREVKKERSRDQGMSL

  Protein domains


Predicted by InterproScan.

(1-193)


  Protein structure


Source ID Structure
AlphaFold DB F5X340


Host bacterium


ID   16592 GenBank   NZ_CP044319
Plasmid name   15-307-1|p15-307-1_03 Incompatibility group   -
Plasmid size   46329 bp Coordinate of oriT [Strand]   8929..8966 [+]
Host baterium   Enterococcus faecium strain 15-307-1

Cargo genes


Drug resistance gene   tet(M), tet(L)
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -