Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 116142 |
| Name | oriT_59458|p59458-1  |
| Organism | Staphylococcus aureus strain 59458 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_AP024724 (25037..25072 [+], 36 nt) |
| oriT length | 36 nt |
| IRs (inverted repeats) | 4..10, 14..20 (GCGAACG..CGTTCGC) |
| Location of nic site | 29..30 |
| Conserved sequence flanking the nic site |
GTGCGCCCTT |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 36 nt
>oriT_59458|p59458-1
CACGCGAACGGAACGTTCGCATAAGTGCGCCCTTAC
CACGCGAACGGAACGTTCGCATAAGTGCGCCCTTAC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 10324 | GenBank | WP_001252101 |
| Name | MobA_MobL_OCX22_RS14340_59458|p59458-1 |
UniProt ID | A0A141HMI9 |
| Length | 665 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 665 a.a. Molecular weight: 78908.86 Da Isoelectric Point: 5.3910
>WP_001252101.1 MULTISPECIES: MobA/MobL family protein [Bacilli]
MAMYHFQNKFVSKANGQSATAKSAYNSASRIKDFKENEFKDYSNKQCDYSEILLPNNADDKFKDREYLWN
KVHDVENRKNSQVAREIIIGLPNEFDPNSNIELAKEFAESLSNEGMIVDLNIHKINEENPHAHLLCTLRG
LDKNNEFEPKRKGNDYIRDWNTKEKHNEWRKRWENVQNKHLEKNGFSVRVSADSYKNQNIDLEPTKKEGW
KARKFEDETGKKSSISKHNESIKKRNQQKIDKMFNEVDDMKSHKLNAFSYMNKSDSTTLKNMAKDLKIYV
TPINMYKENERLYDLKQKTSLITDDEDRLNKIEDIEDRQKKLESINEVFEKQAGIFFDKNYPDQSLNYSD
DEKIFITRTILNDRDVLPANNELEDIVKEKRIKEAQISLNTVLGNRDISLESIEKESNFFADKLSNILEK
NNLSFDDVLENKHEGMEDSLKIDYYTNKLEVFRNAENILEDYYDVQIKELFTDDEDYKAFNEVTDIKEKQ
QLIDFKTYHGTENTIEMLETGNFIPKYSDEDRKYITEQVKLLQEKEFKPNKNQHDKFVFGAIQKKLLSEY
DFDYSDNNDLKHLYQESNEVGDEISKDNIEEFYEGNDILVDKETYNNYNRKQQAYGLINAGLDSMIFNFN
EIFRERMPKYINHQYKGKNHSKQRHELKNKRGMHL
MAMYHFQNKFVSKANGQSATAKSAYNSASRIKDFKENEFKDYSNKQCDYSEILLPNNADDKFKDREYLWN
KVHDVENRKNSQVAREIIIGLPNEFDPNSNIELAKEFAESLSNEGMIVDLNIHKINEENPHAHLLCTLRG
LDKNNEFEPKRKGNDYIRDWNTKEKHNEWRKRWENVQNKHLEKNGFSVRVSADSYKNQNIDLEPTKKEGW
KARKFEDETGKKSSISKHNESIKKRNQQKIDKMFNEVDDMKSHKLNAFSYMNKSDSTTLKNMAKDLKIYV
TPINMYKENERLYDLKQKTSLITDDEDRLNKIEDIEDRQKKLESINEVFEKQAGIFFDKNYPDQSLNYSD
DEKIFITRTILNDRDVLPANNELEDIVKEKRIKEAQISLNTVLGNRDISLESIEKESNFFADKLSNILEK
NNLSFDDVLENKHEGMEDSLKIDYYTNKLEVFRNAENILEDYYDVQIKELFTDDEDYKAFNEVTDIKEKQ
QLIDFKTYHGTENTIEMLETGNFIPKYSDEDRKYITEQVKLLQEKEFKPNKNQHDKFVFGAIQKKLLSEY
DFDYSDNNDLKHLYQESNEVGDEISKDNIEEFYEGNDILVDKETYNNYNRKQQAYGLINAGLDSMIFNFN
EIFRERMPKYINHQYKGKNHSKQRHELKNKRGMHL
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A141HMI9 |
T4CP
| ID | 12070 | GenBank | WP_240562779 |
| Name | t4cp2_OCX22_RS14275_59458|p59458-1 |
UniProt ID | _ |
| Length | 546 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 546 a.a. Molecular weight: 62692.64 Da Isoelectric Point: 7.6577
>WP_240562779.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Staphylococcus]
MTTLLGELESKATSINKKNLVIQDFDTKCPNRNIFVVGGPGSYKSAGYVIPNVIVKNQQSIVVTDPSGEV
YEKTANIKRMQGFDVRVVNFKNFLASDRQNFFDYIDKDSDCSIVAELIIKSAGDSKINKDVWYKASVGLL
NSLLLYARYEFEPEKRTIGNIIKFIQNNKPNQDDEGSVELDNRFNELPKDHPARESYEFGFAVSEGRTRA
SIILTFVADLRNYIDNEIRSYTSDSDFDLRDVGLRKTIIYVMLPVLGNTVQSLSSLFFSQMFQQLYRLGD
ENGARLPVPVDFLLDEWPNIGAIPDYEETLATCRKYGISITTIVQSISQAVDKYNKDKANAIIGNHAVIL
CLGNVNNDTAKYIKEELGNATVEFETSSEGSSSGKDSRSKSSNKNVSYTGRALLNEDEISNMQQDKAILI
TKNRNPKIIKKLAYFKMFPNLTETYIAPQNEYKRKKNQSMIDKHNRLREEWNKKQERIKQQKLEEYKEEQ
RQKELEKEQQPQEEQQNEKVKESKDKNDQEDKKDEQQKINDSKKAFYEKLKQKQAK
MTTLLGELESKATSINKKNLVIQDFDTKCPNRNIFVVGGPGSYKSAGYVIPNVIVKNQQSIVVTDPSGEV
YEKTANIKRMQGFDVRVVNFKNFLASDRQNFFDYIDKDSDCSIVAELIIKSAGDSKINKDVWYKASVGLL
NSLLLYARYEFEPEKRTIGNIIKFIQNNKPNQDDEGSVELDNRFNELPKDHPARESYEFGFAVSEGRTRA
SIILTFVADLRNYIDNEIRSYTSDSDFDLRDVGLRKTIIYVMLPVLGNTVQSLSSLFFSQMFQQLYRLGD
ENGARLPVPVDFLLDEWPNIGAIPDYEETLATCRKYGISITTIVQSISQAVDKYNKDKANAIIGNHAVIL
CLGNVNNDTAKYIKEELGNATVEFETSSEGSSSGKDSRSKSSNKNVSYTGRALLNEDEISNMQQDKAILI
TKNRNPKIIKKLAYFKMFPNLTETYIAPQNEYKRKKNQSMIDKHNRLREEWNKKQERIKQQKLEEYKEEQ
RQKELEKEQQPQEEQQNEKVKESKDKNDQEDKKDEQQKINDSKKAFYEKLKQKQAK
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 1833..13047
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| OCX22_RS14220 (SA59458_27100) | 105..293 | - | 189 | WP_156978554 | regulator | - |
| OCX22_RS14225 (SA59458_27110) | 465..1439 | + | 975 | WP_240562792 | conjugative transfer protein TrsA | - |
| OCX22_RS14230 (SA59458_27120) | 1456..1773 | + | 318 | WP_000591622 | CagC family type IV secretion system protein | - |
| OCX22_RS14235 (SA59458_27130) | 1833..2240 | + | 408 | WP_000110157 | hypothetical protein | trsC |
| OCX22_RS14240 (SA59458_27140) | 2227..2902 | + | 676 | Protein_4 | TrsD/TraD family conjugative transfer protein | - |
| OCX22_RS14245 (SA59458_27160) | 2923..4935 | + | 2013 | WP_000874919 | VirB4 family type IV secretion system protein | virb4 |
| OCX22_RS14250 (SA59458_27170) | 4947..6227 | + | 1281 | WP_156982569 | hypothetical protein | trsF |
| OCX22_RS14255 (SA59458_27180) | 6245..7321 | + | 1077 | WP_156982570 | CHAP domain-containing protein | trsG |
| OCX22_RS14260 (SA59458_27190) | 7332..7817 | + | 486 | WP_012896712 | TrsH/TraH family protein | - |
| OCX22_RS14265 (SA59458_27200) | 7833..9935 | + | 2103 | WP_240576050 | DNA topoisomerase III | - |
| OCX22_RS14270 (SA59458_27210) | 9951..10415 | + | 465 | WP_221548463 | hypothetical protein | trsJ |
| OCX22_RS14275 (SA59458_27220) | 10412..12052 | + | 1641 | WP_240562779 | VirD4-like conjugal transfer protein, CD1115 family | - |
| OCX22_RS14280 (SA59458_27230) | 12130..13047 | + | 918 | WP_240562780 | conjugal transfer protein TrbL family protein | trsL |
| OCX22_RS14285 (SA59458_27240) | 13064..13456 | + | 393 | WP_240562782 | single-stranded DNA-binding protein | - |
| OCX22_RS14290 (SA59458_27250) | 13513..14244 | + | 732 | WP_240562783 | hypothetical protein | - |
| OCX22_RS14295 (SA59458_27260) | 14281..14901 | + | 621 | WP_261921412 | hypothetical protein | - |
| OCX22_RS14300 (SA59458_27270) | 14920..15534 | + | 615 | WP_000527296 | hypothetical protein | - |
| OCX22_RS14305 (SA59458_27280) | 15603..16874 | + | 1272 | WP_156982554 | hypothetical protein | - |
| OCX22_RS14310 (SA59458_27290) | 16916..17332 | - | 417 | WP_156982555 | YolD-like family protein | - |
Host bacterium
| ID | 16575 | GenBank | NZ_AP024724 |
| Plasmid name | 59458|p59458-1 | Incompatibility group | - |
| Plasmid size | 29077 bp | Coordinate of oriT [Strand] | 25037..25072 [+] |
| Host baterium | Staphylococcus aureus strain 59458 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |