Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 116141
• Name: oriT_pKAM497_3
• Organism: Aeromonas caviae strain KAM497
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_AP026378 (5564..5700 [-], 137 nt)
• oriT length: 137 nt
• IRs (inverted repeats): 28..33, 37..42 (ACATGC..GCATGT)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 137 nt

>oriT_pKAM497_3
CGAATGGGCAGGCGGGGATTGGCAGGAACATGCGTAGCATGTGTATGACAATCCGCATCCTGTATTGCTCAACCACTCTACTATCATATCAGCAGTTTAGGTGCACGGGACGCACTTTGCAACAAGCATAAAAGGCG

oriT secondary structure

Predicted by RNAfold.

Relaxase

traI_OCZ21_RS22705_pKAM497_3

• ID: 10323
• GenBank: WP_261950819
• Name: traI_OCZ21_RS22705_pKAM497_3
• UniProt ID: _
• Length: 814 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 814 a.a.
• Molecular weight: 93491.76 Da
• Isoelectric Point: 9.6502

>WP_261950819.1 TraI/MobA(P) family conjugative relaxase [Aeromonas caviae]
MSWREGEQPSPEQVEEAVSIFMDELGWKDHQAIYGLHSDTDNIHLHIVINRVHPETLKIVEKNRGFDIEL
AHKAIARIEHAQGWQREQNGRYQVLENGELGRAPYDPEKPRQPDQKKRDMENRTGEKSAHRIAIEDGAAI
IKQAQTWEQLHRELAAKGMRYEKTGSGATVFVGDVGVKASDVDRNASLAKMQKRLGEYQPAPQRQQVAPR
EPEPIKPDVPGWKDYITGRKAHYAEKNADKLAQDKRQEQERKQLAEQQKARRDELMRGNWKGKGEVLNAM
RSVIAAEQAAEKAALKEKHQKEREQHRQRFRPYPDLEQWQRMQKSPELAEQWRHRASEPQRIEGDRSEPP
TPRDIRAYQPEIVGQQVHYSRKEEAGRGGGVSFVDKGKSIDIHDWRNRDSTLAALQLSAQKWGSFTVMGN
DEYKAMCGKLAAEHGFKITNPELQESIQQERQRIQQERVQAMKSEQLKQFERYAEAVGAERYRVTSIKMR
EDGSKQTFILDKKDGITRGFTPQEIEQRTPEMQRLQRRGENLYYTPLSDKKHHILIDDMNREKLERLIRD
GYQPAAVLESSPGNYQAIITVPKLGTAHDKDVGNRLSDALNREYGDPKLSGAIHPHRAPGYENRKPKHQR
EDGSYPEVRLLKAERRECIKALALSSQIDAEYQRQAALKAQQPERSKAKPALELAAASGSAIDAYQRHYR
DVLKRQRGGEVDLSRLDSMIAVRMRVTGHDQAAIEGAIRQCAPATRQKDEGRDWNDYAQRTARYAYSAAG
DRQAAELGKYRQQWEKLEGREPVRQQEQAKAQKIERDNSPGMSL

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (2-198)

• Large polyvalent protein-associated domain 7 (LPD7) (363-451)

• RepB DNA-primase from phage plasmid (RepB_primase) (472-731)

Protein structure

No available structure.

Host bacterium

• ID: 16574
• GenBank: NZ_AP026378
• Plasmid name: pKAM497_3
• Incompatibility group: IncQ2
• Plasmid size: 9757 bp
• Coordinate of oriT [Strand]: 5564..5700 [-]
• Host baterium: Aeromonas caviae strain KAM497

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -