Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   116140
Name   oriT_pKAM497_2 in_silico
Organism   Aeromonas caviae strain KAM497
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_AP026377 (11105..11242 [-], 138 nt)
oriT length   138 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 138 nt

>oriT_pKAM497_2
CGAATGGGCAGGCGTCCGTTGGAACCACGTGGCGAAGCCATGTGTGTTACAACGGTCATCCTGTATTGCTCATCCGCTCTACTATCGTATCAGCAGTTTAGGTGCACGGGACGCACTTTGCAACAAGCACAAATGGCG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   10322 GenBank   WP_059385972
Name   traI_OCZ21_RS22530_pKAM497_2 insolico UniProt ID   A0A7I8I4L4
Length   890 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 890 a.a.        Molecular weight: 101396.95 Da        Isoelectric Point: 9.8812

>WP_059385972.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Gammaproteobacteria]
MIVKKVKNPQKAASKAVRVSRLTDYIREPERENSQEKCIHAGARGFITDEPQSQTAEMIALSQEAVRSKD
TINHYVLSWREGEQPSPEQVEEAVSIFMDELGVKDHQAIYGLHADTDNLHLHLAINRVHPETLKVVKINN
GFDIEAAHKAIARIENAQGWQREQNGRYQVLENGELGREHIDKDKPRQPAQPKRDMENRTGEKSAERIAI
EDGAPIIKKAQTWEQLHRELAAKGMRYEKTGSGATLFVGDVGVKASSADRDASLSKLQKRLGAYQPPPQR
QQVAQREPEPIKPDVPGWKDYITGRKAHYAEKNAAKLALDKRQEQERKQIAEQQKARRDELMRGNWKGKG
EVLNAMRSVIAAEQAAEKAALKEKHQKQREQHRQQFRPYPDLEQWQRMQKSPELAEQWRHRASEPQRIEG
ASGEPPTPRDIRAYQPEIVGQQVHYSRKEEAGAGGGVSFVDKGKSIDIHDWRNRDSTLAALQLSAQKWGS
FTVTGNDEYKAMCAKLAAEHGFKITNPELQERIQQERQRIQQERAQAMKSEQLKQFELYAEAVGAERYRV
TSIKMQADGRKQTFILDKKDGITRGFTPQEIEQRTPEMLRLQRRGENLYYTPLSDKKHHILIDDMNREKL
ERLIRDGYRPAVVLESSPGNYQAIITVPKLGTAHDKDVGNRLSDALNREYGDPKLSGAIHPHRAPGYENR
KPKHQREDGSYPEVRLLKAERRECVKALALSSQIDAEYQRQAALKAQQPERSKAKPALELAAASGSAIDA
YQRHYRDVIKRQRGGEVDLSRVDSMIAVRMRVTGHDQAAIEGAIRQCAPATRQKGEGRDWNDYAQRTARY
AYSAAGDRQAAELGKYRQQWEKLEGREPVRQQEQAKAQKIERDNSPGMSR

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(19-273)

Large polyvalent protein-associated domain 7 (LPD7)

(439-527)

RepB DNA-primase from phage plasmid (RepB_primase)

(549-807)


  Protein structure


Source ID Structure
AlphaFold DB A0A7I8I4L4


Host bacterium

ID   16573 GenBank   NZ_AP026377
Plasmid name   pKAM497_2 Incompatibility group   IncP6
Plasmid size   31080 bp Coordinate of oriT [Strand]   11105..11242 [-]
Host baterium   Aeromonas caviae strain KAM497

Cargo genes

Drug resistance gene   blaGES-4, catB8, ant(3'')-Ia, qacE, sul1
Virulence gene   -
Metal resistance gene   merR, merT, merP, merC, merA, merD, merE
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -