Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   116139
Name   oriT_pKAM497_1 in_silico
Organism   Aeromonas caviae strain KAM497
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_AP026376 (76515..76644 [+], 130 nt)
oriT length   130 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 130 nt

>oriT_pKAM497_1
GTTAACCCGTTTGATCGCGTGATGAATGCGCTAAAAAGTCGAGGCCGTAAGAACGCCCACATTCTGAGCATCTTGCAGCTGGATTGGCCTGCGTCTGAATCGATCATCCATCGCCTGAGCGACTACATCA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   10321 GenBank   WP_223936724
Name   mobH_OCZ21_RS21830_pKAM497_1 insolico UniProt ID   _
Length   984 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 984 a.a.        Molecular weight: 107289.59 Da        Isoelectric Point: 4.7871

>WP_223936724.1 MULTISPECIES: MobH family relaxase [Gammaproteobacteria]
MDEEDIPRYPPFAKGLPVAPIDKILATQAELIEKVRNSLGFTIEDFNRLVLPVIHRYAAFVHLLPASEAH
HHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPKQRRDNEPRWRLASCFSGLLHDVGKPLADVSITDKN
GVVTWNPYFESLHDWAGRNHVDRYFIRWREKRHKRHEQFSLLAVDRIIPVATREYLSESGPLIIEAMLEA
ISGTSVNQPVTKLMLRADQESVSRDLKQNRLNVDEFSYGVPVERYVFDAIRRLVKTGKWKVNEPGAKVWH
LQQGVFIAWRNLGDLYDLISHDKIPGIPRDPDTLADILIERGFAVPNTVQEKGDRAYYRYWEVLPEMIQE
AAGSIKILMLRLESNELVFTTEPPAAVAGEVVGDVADAVIELIDPDEASDADDESASGMSESLLSAEQEA
MNALAGLGFGDAMEILGGTASDDNATQPAPVVDTTPPAKPAKGAGGRGGKNQAKKTAPAAPEQQQPEPSP
QEIAKSTPPLAVENPLQAIMDVGGGLGGLDFPFDAFVAGTSMSDEPSQALAPEPKQDVANPDNPDTPNLG
LMNQVFPNFMNDESTGDVGGMDLPPAWAIEEIPSFHAYEGEVGQPGNQFVSEELAGADAPDAKGELLTLM
ATFGEAGPILERAILPVLEGKATLGEVLCLMKGQAVILYPEGARALGAPSEILTALFHAQAIVPDPVMSG
RKVQDFNGVKAIVLTEQLSSAFIAAIKQAESLLGGYQDAFELVASPEIKKTQSKKNRGQRKTSRSESSGE
DSHAASPERAQVEEKRVIADAQLIDVSVEPPSPPVDTTPPPDKVVPRKESRGKEKVDKPIQPAPTTKPAE
GTQNLARLPPREQPKPESQQKQARVIDVGNIALPGPDLPEELSPRSIVKEETVDEALYLPPKMTPEKAIQ
MLKEMIKNRSGRWLVSAVLEEEGFLTTSDKAFEVIAGEYTGISKHVLGGTLRRAQRRPLLKQRQGKLYLE
VDKS

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(22-328)


  Protein structure



No available structure.




T4CP

ID   12068 GenBank   WP_011899407
Name   traD_OCZ21_RS21835_pKAM497_1 insolico UniProt ID   _
Length   621 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 621 a.a.        Molecular weight: 68931.34 Da        Isoelectric Point: 6.8339

>WP_011899407.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Gammaproteobacteria]
MTYDPLAYEMPWRPNYEMSAVFGWVAASVGALAVQQITDMPPEPFYWMTGICGVMTMARLPKAIKLHLLQ
KHLKGRDLEFISIKELQKKVQASPEDMWLGYGFAWENRHAQRVFEILKRDWTSIVGTESTTSKIVRTIRG
KKKKEVPIGQPWIHGVEPKEEHLMQPLKHTEGHSLIVGTTGSGKTRMFDTLISQAILRGEAVIIIDPKGD
KEMRDNARRACEAMGQAERFVSFHPAFPEESVRIDPLRNFTRVTEIASRLAALIPSEGGGDPFKSFGWQA
LNNIAQGLILAYDRPNLIKLRRFLEGGADGLVIKAVQAYAERVMPDWEAAAAPYLEKAANGSRSKLAVAH
MAFYNNVIQPEHPSSELEGLLNMFKHDATHFSKMVSNLLPIMNMLTSGELGPLLSPDATDLSDERQITDS
AKIINNAQVAYLGLDSLTDNMVGSALGSIFLSDLTAVAGDRYNYGVNNLPVNIFVDEAAEVINDPFIQLL
NKGRGAKLRLFVATQTFADFAARLGSKDKALQVLGNINNVFALRIVDAETQEYIAENLPKTRLKYVMRTQ
GQNTDGSEPIMHGGNQGERLMEEEADLFPAQLLGMLPNLEYIAKISGGKIVKGRLPILTEK

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(470-597)

AAA-like domain (AAA_10)

(168-257)

  Protein structure



No available structure.



ID   12069 GenBank   WP_081374866
Name   traC_OCZ21_RS21920_pKAM497_1 insolico UniProt ID   _
Length   814 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 814 a.a.        Molecular weight: 91684.72 Da        Isoelectric Point: 5.7286

>WP_081374866.1 MULTISPECIES: type IV secretion system protein TraC [Gammaproteobacteria]
MIVTIKKKLDGALIQEHMRASGVIPVLAYSEDEQVFLMDDQSAGFGFICEPMCGADEKVQERMNGFLNQE
FPSKTTLQFLLFRSPDINQEMYQMLGLRDGFRHGLLSPVIQERINYLQHHTTERIMARTNKGIYDNGLIQ
DLKLFVTCKVPIKSNTPTETELVELDQLKTKIESSLQTVGLRPRTMTAVNYVRIMSTLLNWGPDASWRHD
ASRWETDKPICEQLFDYGTDLEVSKNGIRLGGYHAKVLSAKKLPDIAYFGDAVAYTGDLTGGNSSIKENY
MVVTNVFFPEVESTKNTLERKRQFTVNQAYGPMLKFVPVLADKKESFDTVYESLKEGAKPVKISYSLVIF
APTKERVESAATAARSIWRESRFELMEDRFVALPIFLNSLPFCTDRDAVRDLFRYKTMTSEQAAVLLPVF
GEWKGTGTFHAALISRNGQLMSLSLHDSNTNKNLVIAAESGSGKSFLTNELICSYLSEGAQVWVIDAGKS
YQKMSEMLRGDFVHFEEGTHVCLNPFELIQNYEDEEDAIVSLVCAMASSKGLLVEWQISALKQVMSSIWE
EKGTTMKVDDIAERCLEGEDLRLKDVGQQLYAFTSEGSYGKYFSNKNNVSFQNQFTVLELDELQGRKHLR
QVVLLQLIYQIQQEVFLGERNRKKVVIVDEAWDLLKEGEVSVFMEHAYRKFRKYGGSVVIATQSINDLYE
NAVGRAIAENSASMYLLGQTEETVESVKRAGRLALSDGGFHTLKTVHTIQGVYSEIFIKSKSGMGVGRLI
VGDFQKLLYSTDPVDVNAIDQFVNQGLSVPDAIKAVMQARRQAA

  Protein domains


Predicted by InterproScan.

TraG P-loop domain (P-loop_TraG)

(449-808)

Family of unknown function (DUF5934) (DUF5934)

(284-434)

TraC protein (TraC_F_IV)

(21-255)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 74594..101220

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OCZ21_RS21825 (KAM497c_42150) 70773..71387 - 615 WP_011899409 hypothetical protein -
OCZ21_RS21830 (KAM497c_42160) 71637..74591 + 2955 WP_223936724 MobH family relaxase -
OCZ21_RS21835 (KAM497c_42170) 74594..76459 + 1866 WP_011899407 conjugative transfer system coupling protein TraD virb4
OCZ21_RS21840 (KAM497c_42180) 76470..77054 + 585 WP_011899406 hypothetical protein -
OCZ21_RS21845 (KAM497c_42190) 77011..77640 + 630 WP_011899405 DUF4400 domain-containing protein tfc7
OCZ21_RS21850 (KAM497c_42200) 77786..78178 + 393 WP_043774535 hypothetical protein -
OCZ21_RS21855 78356..78883 + 528 WP_139027008 hypothetical protein -
OCZ21_RS21860 (KAM497c_42210) 79321..79749 + 429 WP_011899394 hypothetical protein -
OCZ21_RS21865 (KAM497c_42220) 79761..79988 + 228 WP_011899395 hypothetical protein -
OCZ21_RS21870 (KAM497c_42230) 80022..80618 + 597 WP_151884378 hypothetical protein -
OCZ21_RS21875 (KAM497c_42240) 80667..80912 + 246 WP_011899397 hypothetical protein -
OCZ21_RS21880 (KAM497c_42250) 81454..81735 + 282 WP_011899398 type IV conjugative transfer system protein TraL traL
OCZ21_RS21885 (KAM497c_42260) 81732..82367 + 636 WP_011899399 TraE/TraK family type IV conjugative transfer system protein traE
OCZ21_RS21890 (KAM497c_42270) 82351..83256 + 906 WP_059119365 type-F conjugative transfer system secretin TraK traK
OCZ21_RS21895 (KAM497c_42280) 83259..84578 + 1320 WP_139027009 TraB/VirB10 family protein traB
OCZ21_RS21900 (KAM497c_42290) 84575..85141 + 567 WP_011899401 type IV conjugative transfer system lipoprotein TraV traV
OCZ21_RS21905 (KAM497c_42300) 85232..85558 + 327 WP_050804919 TraA family conjugative transfer protein -
OCZ21_RS21910 (KAM497c_42310) 85740..91226 + 5487 WP_223936723 Ig-like domain-containing protein -
OCZ21_RS21915 (KAM497c_42320) 91382..92107 + 726 WP_223936722 DsbC family protein trbB
OCZ21_RS21920 (KAM497c_42330) 92104..94548 + 2445 WP_081374866 type IV secretion system protein TraC virb4
OCZ21_RS21925 (KAM497c_42340) 94579..94896 + 318 WP_151884429 hypothetical protein -
OCZ21_RS21930 (KAM497c_42350) 94893..95423 + 531 WP_073545150 S26 family signal peptidase -
OCZ21_RS21935 (KAM497c_42360) 95386..96651 + 1266 WP_073545151 TrbC family F-type conjugative pilus assembly protein traW
OCZ21_RS21940 (KAM497c_42370) 96648..97319 + 672 WP_223936720 EAL domain-containing protein -
OCZ21_RS21945 97316..97663 + 348 WP_261950815 TraU family protein traU
OCZ21_RS21950 (KAM497c_42380) 97657..98436 + 780 Protein_93 TraU family protein -
OCZ21_RS21955 (KAM497c_42390) 98446..101220 + 2775 WP_223936719 conjugal transfer mating pair stabilization protein TraN traN
OCZ21_RS21960 (KAM497c_42400) 102778..104052 - 1275 WP_045892401 IS4-like element ISApu2 family transposase -
OCZ21_RS21965 (KAM497c_42410) 104209..104580 + 372 WP_004099020 hypothetical protein -
OCZ21_RS21970 (KAM497c_42420) 104804..106078 + 1275 WP_011191341 IS4-like element ISApu2 family transposase -

Host bacterium

ID   16572 GenBank   NZ_AP026376
Plasmid name   pKAM497_1 Incompatibility group   -
Plasmid size   192599 bp Coordinate of oriT [Strand]   76515..76644 [+]
Host baterium   Aeromonas caviae strain KAM497

Cargo genes

Drug resistance gene   -
Virulence gene   hcp, vgrG1
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -