Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 116137
• Name: oriT_59731|p59731-1
• Organism: Staphylococcus aureus strain 59731
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_AP024739 (26615..26803 [+], 189 nt)
• oriT length: 189 nt
• IRs (inverted repeats): 149..155, 162..168 (CTATCAT..ATGATAG) 132..138, 142..148 (GTCTGGC..GCCAGAC) 71..76, 89..94 (AAAAAT..ATTTTT) 33..38, 45..50 (TGTCAC..GTGACA)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 189 nt

>oriT_59731|p59731-1
TGTCACAAAAATAAGACAAAATCTACATGTAGTGTCACAAAACCGTGACAAACACAATATATAGTGTCACAAAAATGTGACACTAGGTATTTTTATGATATCACTATGAAATGTCCTAAAACCCTTGGAATGTCTGGCTTTGCCAGACCTATCATTGTACGATGATAGCAAATTCCCCTTATGCTCTTA

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobP2_OCX20_RS14380_59731|p59731-1

• ID: 10320
• GenBank: WP_042742193
• Name: mobP2_OCX20_RS14380_59731|p59731-1
• UniProt ID: A0A1B3ISX3
• Length: 382 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 382 a.a.
• Molecular weight: 45794.09 Da
• Isoelectric Point: 9.8763

>WP_042742193.1 MobP2 family relaxase [Staphylococcus aureus]
MPKIVTVSQFEQPNEFYEYSNYVNYMKRGEAQNNKDDFKYDVYAHYMFDEEKSQNMFNEHDNFMSKKAIN
KIKSDFSHAQKNKGMMWKDVISFDNSSLESVGIYDSKTHTLDEQKIKEATRKMMKRFEKKEGLEDNLVWS
GAIHYNTDNIHVHVAAVEKVIKRTRGKRKPATLKHMKSTFANELFDIKGERQKINEFIRERIVKGIRDES
EPCKDKAMKKQIKKVHKLLEDIPKNQWNYNNNILKYIRPEIDKITDIYIQNYHSKDFEVFKNDLKRQNNL
YKETYGENSNYGSYEKTKMKDLYARSGNTILKNIKELDQDLMKVKQQFTHSKLNVTITKLKIGKCLNQAL
YRTKYALRSDYDKEKNIQQYYQEFDKQSYRER

Protein domains

Predicted by InterproScan.

• MobL relaxases (MobL) (1-350)

Protein structure

• AlphaFold DB: A0A1B3ISX3

T4CP

t4cp2_OCX20_RS14330_59731|p59731-1

• ID: 12067
• GenBank: WP_235854108
• Name: t4cp2_OCX20_RS14330_59731|p59731-1
• UniProt ID: _
• Length: 857 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 857 a.a.
• Molecular weight: 98676.15 Da
• Isoelectric Point: 6.2583

>WP_235854108.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Staphylococcus]
MKQLNTKYTDYIFNFRSDAVLLYIPIWVLVFIFFGKKVYQFRQRFKSLNNNEKASGRFATRKEITQQYKA
IPQREHAFEGEGGLPVAMFGVYDFVTKTKQYLEQRKTAIREINEITDLTADEKLELRKDKLGKMNKEFAT
TFFSKREFTFIDESPTNNLIVGTSRSGKGEIVVLSMIENYSRSSEQPSLIVNDMKGELFSASYKTLENRG
YQVEIFNLDQPMESTMSFNPLQQVIDEYMKGDLGEAQEMTKQITYTLTNNEGVEDNEWNLMAGALINAMI
LALCEETLPKNPEKVTLYSVSNMLQTLSTKRFYKEIAIGNKVQLIEVSALDEYMERFPSHSPAKTQYATV
QAAPDKMRSSIIGTALKALQPFTTDTIAKLTSHTSFDINKLGFPSIIDGYATKDSTFELGVQVLRDGENG
IEYEEVEGIQIGVNEYGYWQYPFKTVLKVGDRIETIEKDENNQVITSYFYVEHIDDKGQVAFNQKGELDN
SDITIRKFNSFPKPIAVFMVVPDYNNANHGIASILVNQIVFEISKNSQLYTENQSTYRRVIFHLDEAGNM
PPIPNLSQKVNVSLSRGLRFNFFVQAFSQFKDKYGDAYDAIMDACQNKVYIMATQEQTLKDFSAMIGSQQ
ITVHSRSGEAGAIKSSITENQEERPLLRPDELARLQEGETVVVRNLKRQDKKRNKVMSYPIFNDGKYAMK
YRYQYLSDLMDTSQSIIHFRPMLKARCEHRHLQLEATLVDWDKLIEEMQEGIDGQKEENKNINDINQYKK
SNEEGNVAVRVKRLETLKDKVGDAVFDKITRRFERYLRTYAEQGKNVNTITQKKLEELVLADSEVKEEEK
TKRIEMIKKLIKEAKTT

Protein domains

Predicted by InterproScan.

• TraM recognition site of TraD and TraG (TraG-D_C) (549-666)

• Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf) (143-392)

Protein structure

No available structure.

Host bacterium

• ID: 16570
• GenBank: NZ_AP024739
• Plasmid name: 59731|p59731-1
• Incompatibility group: -
• Plasmid size: 40197 bp
• Coordinate of oriT [Strand]: 26615..26803 [+]
• Host baterium: Staphylococcus aureus strain 59731

Cargo genes

• Drug resistance gene: aac(6')-aph(2'')
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: AcrIIA21, AcrIIA14