Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   116120
Name   oriT_2022CK-00409|unnamed6 in_silico
Organism   Enterobacter cloacae strain 2022CK-00409
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP104842 (166910..167060 [-], 151 nt)
oriT length   151 nt
IRs (inverted repeats)      93..98, 106..111  (TGGCCT..AGGCCA)
 12..17, 24..29  (AACCCT..AGGGTT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 151 nt

>oriT_2022CK-00409|unnamed6
TGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   10307 GenBank   WP_261659477
Name   mobH_N7S94_RS28790_2022CK-00409|unnamed6 insolico UniProt ID   _
Length   803 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 803 a.a.        Molecular weight: 88000.07 Da        Isoelectric Point: 4.6065

>WP_261659477.1 MobH family relaxase [Enterobacter cloacae]
MLKALNKLFGGRSGVIETAPSARVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEADDGDDQEEGEAALNDDMLAAEQEAEKALAGLGFGDAMDMLKSTSDAVEEKPEQKDAGPTESSK
PDAGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPF
DAFNASAETTSTDATNSEIPDVAMPGKQEEQPKQDFVPQEQNSLQGDDFPMFGGSDEPPSWAIEPLPMLT
DAPEQPTHTPEMPHTDNVNQHEKDAKTLLVEMLSGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVI
LYPDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQ
DAFELVSPPGLDASKNKSAPKQQSRKKGAAAEA

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(51-357)


  Protein structure



No available structure.




T4CP

ID   12057 GenBank   WP_000637386
Name   traC_N7S94_RS28705_2022CK-00409|unnamed6 insolico UniProt ID   _
Length   815 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 815 a.a.        Molecular weight: 92590.87 Da        Isoelectric Point: 5.9222

>WP_000637386.1 MULTISPECIES: type IV secretion system protein TraC [Gammaproteobacteria]
MIVTIKKKLEETLIPEHLRAAGIIPVLAYDEDDHVFLMDDHSAGFGFMCEPLCGADEKVQERMNGFLNQE
FPSKTTLQFVLFRSPDINQEMYRMMGLRDGFRHELLTSVIKERINFLQHHTTERIFAKTNKGIYDNGLIQ
DLKLFVTCKVPIKNNNPTESELQQLAQLRTKVESSLQTVGLRPRTMTAVNYIRIMSTILNWGPDASWRHD
SVDWEMDKPICEQIFDYGTDVEVSKNGIRLGDYHAKVMSAKKLPDVFYFGDALTYAGDLSGGNSSIKENY
MVVTNVFFPEAESTKNTLERKRQFTVNQAYGPMLKFVPVLADKKESFDTLYESMKEGAKPVKITYSVVLF
APTKERVEAAAMAARNIWRESRFELMEDKFVALPMFLNCLPFCTDRDAVRDLFRYKTMTTEQAAVVLPVF
GEWKGTGTYHAALISRNGQLMSLSLHDSNTNKNLVIAAESGSGKSFLTNELIFSYLSEGAQVWVIDAGKS
YQKLSEMLNGDFVHFEEGTHVCLNPFELIQNYEDEEDAIVSLVCAMASAKGLLDEWQISALKQVLSRLWE
EKGKEMKVDDIAERCLEEENDQRLKDIGQQLYAFTSKGSYGKYFSRKNNVSFQNQFTVLELDELQGRKHL
RQVVLLQLIYQIQQEVFLGERNRKKVVIVDEAWDLLKEGEVSVFMEHAYRKFRKYGGSVVIATQSINDLY
ENAVGRAIAENSASMYLLGQTEETVESVKRSGRLTLSEGGFHTLKTVHTIQGVYSEIFIKSKSGMGVGRL
IVGDFQKLLYSTDPVDVNAIDQFVKQGMSIPEAIKAVMRSRQQAA

  Protein domains


Predicted by InterproScan.

TraG P-loop domain (P-loop_TraG)

(449-809)

Family of unknown function (DUF5934) (DUF5934)

(284-434)

TraC protein (TraC_F_IV)

(21-255)

  Protein structure



No available structure.



ID   12058 GenBank   WP_000178856
Name   traD_N7S94_RS28780_2022CK-00409|unnamed6 insolico UniProt ID   _
Length   621 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 621 a.a.        Molecular weight: 69197.66 Da        Isoelectric Point: 6.9403

>WP_000178856.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Gammaproteobacteria]
MTMSYDPLAYEMPWRPNYEKNAVAGWLAASGAALAVEQVSTMPPEPFYWMTGICGVMAMARLPKAIKLHL
LQKHLKGRDLEFISIAELQKYIKDTPDDMWLGSGFLWENRHAQRVFEILKRDWTSIVGRESTVKKVVRKI
QGKKKELPIGQPWIHGVEPKEEKLMQPLKHTEGHSLIVGTTGSGKTRMFDILISQAILRGEAVIIIDPKG
DKEMRDNARRACEAMGQPERFVSFHPAFPEESVRIDPLRNFTRVTEIASRLAALIPSEAGADPFKSFGWQ
ALNNIAQGLVITHDRPNLTKLRRFLEGGAAGLVIKAVQAYSERVMPDWEAEAAAYLEKAKNGSREKIAFA
LMKFYYDIIQPEHPNSDLEGLLSMFQHDQTHFSKMVANLLPIMNMLTSGELGPLLSPDSSDLSDERQITD
SAKIINNAQVAYLGLDSLTDNMVGSAMGSIFLSDLTAVAGDRYNYGVNNRPVNIFVDEAAEVINDPFIQL
LNKGRGAKLRLFVATQTFADFAARLGSKDKALQVLGNINNTFALRIVDGETQEYIADNLPKTRLKYVMRT
QGQNSDGKEPIMHGGNQGERLMEEEADLFPAQLLGMLPNLEYIAKISGGTIVKGRLPILTQ

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(471-598)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(170-297)

  Protein structure



No available structure.



ID   12059 GenBank   WP_104457263
Name   t4cp2_N7S94_RS28925_2022CK-00409|unnamed6 insolico UniProt ID   _
Length   701 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 701 a.a.        Molecular weight: 77343.46 Da        Isoelectric Point: 10.0162

>WP_104457263.1 MULTISPECIES: conjugal transfer protein TraG [Enterobacterales]
MRGGRILWGQIAVVITIVLVMTWAATQWVAFRLGFQPQLGAPWFDLAGLPVYYPPAFFWWWFSFDAYAPA
IFVEGGIIAVSGGFIAIAAAILMSIIRAREARNIATYGSARWAEDKEIRAAGLLGPDGVVLGRHTQDYLR
HDGPEHVLCFAPTRSGKGVGLVVPTLLTWPASAIVHDIKGENWTLTAGFRAKHGRVLLFDPTNAGSSAYN
PLLEVRRGEWEVRDVQNIADILVDPEGSLDKRNHWEKTSHSLLVGAILHVLYAERDKTLAGVANFLSDPR
RPVEATLRAMMDTPHLGEAGVHPVIASSARELLNKSENERSGVLSTAMSFLGLYRDPVVARVTARCDWRI
ADLVGSRRPVTLYLVVPPSDINRTKPLIRLILNQIGRRLTEELTTSGKRHRLLLMLDEFPALGRLDFFES
ALAFMAGYGIKGFLIAQSLNQIERAYGPNNAILDNCHVRVSFATNDERTAKRVSDALGAATELRDSTNYA
GHRLAPWLGHLMVSRQETARPLLTPGEIMQLPPTDEIVMVAGTPPIRATKARYFEDARFQERILTPPKLV
AAPLTSSPLDFIARLAALVPKPRVNLTRFHGVFAPNSRHRALVTPAKRGRGNKVRVADEPATPAQRRASM
TWAQRLKRVFNIDIETCSGCGGAMKVIACIEDPIVIKQILDHLKHKAETSGTRALPESRAPPAELLLGLF
D

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(111-557)

Putative transposase (Y2_Tnp)

(561-597)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 143785..168976

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N7S94_RS28645 (N7S94_28635) 138912..139820 - 909 WP_000739139 hypothetical protein -
N7S94_RS28650 (N7S94_28640) 139831..140799 - 969 WP_000085162 AAA family ATPase -
N7S94_RS28655 (N7S94_28645) 141019..141204 - 186 WP_001186917 hypothetical protein -
N7S94_RS28660 (N7S94_28650) 141508..141828 - 321 WP_000547566 hypothetical protein -
N7S94_RS28665 (N7S94_28655) 142122..142763 + 642 WP_000796664 hypothetical protein -
N7S94_RS28670 (N7S94_28660) 142886..143746 + 861 WP_000709517 hypothetical protein -
N7S94_RS28675 (N7S94_28665) 143785..146583 - 2799 WP_001256487 conjugal transfer mating pair stabilization protein TraN traN
N7S94_RS28680 (N7S94_28670) 146699..147703 - 1005 WP_043940352 TraU family protein traU
N7S94_RS28685 (N7S94_28675) 147703..148374 - 672 WP_001337754 EAL domain-containing protein -
N7S94_RS28690 (N7S94_28680) 148371..149636 - 1266 WP_000621288 TrbC family F-type conjugative pilus assembly protein traW
N7S94_RS28695 (N7S94_28685) 149599..150129 - 531 WP_001010738 S26 family signal peptidase -
N7S94_RS28700 (N7S94_28690) 150126..150443 - 318 WP_000351984 hypothetical protein -
N7S94_RS28705 (N7S94_28695) 150458..152905 - 2448 WP_000637386 type IV secretion system protein TraC virb4
N7S94_RS28710 (N7S94_28700) 152902..153609 - 708 WP_001259347 DsbC family protein -
N7S94_RS28715 (N7S94_28705) 153758..159289 - 5532 WP_000606833 Ig-like domain-containing protein -
N7S94_RS28720 (N7S94_28710) 159490..159882 - 393 WP_000479535 TraA family conjugative transfer protein -
N7S94_RS28725 (N7S94_28715) 159886..160464 - 579 WP_000793435 type IV conjugative transfer system lipoprotein TraV traV
N7S94_RS28730 (N7S94_28720) 160461..161774 - 1314 WP_024131605 TraB/VirB10 family protein traB
N7S94_RS28735 (N7S94_28725) 161774..162691 - 918 WP_000794249 type-F conjugative transfer system secretin TraK traK
N7S94_RS28740 (N7S94_28730) 162675..163301 - 627 WP_001049716 TraE/TraK family type IV conjugative transfer system protein traE
N7S94_RS28745 (N7S94_28735) 163298..163579 - 282 WP_000805625 type IV conjugative transfer system protein TraL traL
N7S94_RS28750 (N7S94_28740) 163724..164089 - 366 WP_001052531 hypothetical protein -
N7S94_RS28755 (N7S94_28745) 164425..165087 - 663 WP_001231463 hypothetical protein -
N7S94_RS28760 (N7S94_28750) 165087..165464 - 378 WP_000869261 hypothetical protein -
N7S94_RS28765 (N7S94_28755) 165474..165920 - 447 WP_000122506 hypothetical protein -
N7S94_RS28770 (N7S94_28760) 165930..166559 - 630 WP_000743450 DUF4400 domain-containing protein tfc7
N7S94_RS28775 (N7S94_28765) 166516..167061 - 546 WP_000228720 hypothetical protein -
N7S94_RS28780 (N7S94_28770) 167111..168976 - 1866 WP_000178856 conjugative transfer system coupling protein TraD virb4
N7S94_RS28785 (N7S94_28775) 168973..169608 - 636 WP_261659476 hypothetical protein -
N7S94_RS28790 (N7S94_28780) 169541..171952 - 2412 WP_261659477 MobH family relaxase -
N7S94_RS28795 (N7S94_28785) 172119..172736 + 618 WP_001249396 hypothetical protein -
N7S94_RS28800 (N7S94_28790) 172718..172951 + 234 WP_001191892 hypothetical protein -

Host bacterium

ID   16553 GenBank   NZ_CP104842
Plasmid name   2022CK-00409|unnamed6 Incompatibility group   IncA/C2
Plasmid size   209995 bp Coordinate of oriT [Strand]   166910..167060 [-]
Host baterium   Enterobacter cloacae strain 2022CK-00409

Cargo genes

Drug resistance gene   blaKPC-2, sul1, qacE, aac(6')-33, ant(2'')-Ia, blaTEM-1D, sul2, floR
Virulence gene   -
Metal resistance gene   merR, merT, merP, merA, merD, merE, merC
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -