Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 116061
• Name: oriT_pSALNBL75
• Organism: Staphylococcus aureus strain B2-7A
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP042047 (19071..19246 [-], 176 nt)
• oriT length: 176 nt
• IRs (inverted repeats): 154..159, 167..172 (AGCATA..TATGCT) 137..144, 148..155 (CTATCATT..AATGATAG) 120..126, 130..136 (GTCTGGC..GCCAGAC)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 176 nt

>oriT_pSALNBL75
TGAGACATATACTATATATAGTGTCACAAAAGTGAGACAACCACTATATATAGTGTCACAAAGGTGAGACAAAAGGGGATTTAAGATATCATTTAAAAATTACCTTGAATTGTTGGAATGTCTGGCTTCGCCAGACCTATCATTATAAATGATAGCATATTCTCCTTATGCTCTTA

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobP2_FP477_RS14135_pSALNBL75

• ID: 10271
• GenBank: WP_145389854
• Name: mobP2_FP477_RS14135_pSALNBL75
• UniProt ID: _
• Length: 391 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 391 a.a.
• Molecular weight: 47186.01 Da
• Isoelectric Point: 9.2044

>WP_145389854.1 MobP2 family relaxase [Staphylococcus aureus]
MPKIVTMSEFEQSKNKTKFSKYVDYVDRDEAKKNEHKEFQYDVYTHYMFDEQKSNSMFNDNSNYMSEDEI
KYTKSKFNEAQKNNGIMWKDVISFDNESLKEAGIFDPECKYLDEQKMRQATRKAMQKFEKKEGLENNLVW
SSSIHYNTDNIHVHVAAVEKDVTRERGKRKYSTIKEMKSSFANELFDMSGERTKINNFIRDRIVKGIKEQ
NEPDYDKQMKLQLKKIHEEIKDIPKKEWQYNNNIMKQARPEIDKFTLMYIKNNHGEEFDEFKQDLKKQSK
LYKETYGDKSDYKKYEETKMNDLYSRAGNTVLKQIKSFNEEYNNIKFKEINKQDNKKPLNINPKVNNKMQ
IGYALNSTLYNTQRALRNDFQKERNINQYHYEFDKSYEKEE

Protein domains

Predicted by InterproScan.

• MobL relaxases (MobL) (1-368)

Protein structure

No available structure.

T4CP

t4cp2_FP477_RS14185_pSALNBL75

• ID: 12005
• GenBank: WP_261374895
• Name: t4cp2_FP477_RS14185_pSALNBL75
• UniProt ID: _
• Length: 941 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 941 a.a.
• Molecular weight: 108637.71 Da
• Isoelectric Point: 8.3862

>WP_261374895.1 VirD4-like conjugal transfer protein, CD1115 family [Staphylococcus aureus]
MIPFRSKHNHGYHRKKASFSQNKHLLAHPIGLLALLVVGFIFIMLAANFFINMLNNLIFAGGDIFDNMKK
INNEWTNYFFKVRFDLALFYIPFFVLLTVLWGKKVYQIRQKFKSLDNQEKASGRFSTKKEIKQQYKAIAQ
REKQFEGEGGLPVAMFGVYDFATKTKQYLEQRKTAKTEINSMPDLTQDEKYNMKKDKLGQINKEFVSTFF
LKREFTFIDESPTNNIFVGTSRSGKGEVFVLSMIENYSRSSHQPSLVVGDMKGELYSASNETLENRNYHT
EVFNLDQPMESTMSFNLLQFVIEEYQKGDLGEAQEMTKQITHTLTNNEGVEDNEWNLMSAALINAMILAL
CEEALPEHPEKVTLYSVSNMLQTLSVKKFYKQIQIGNQVKVIETSALDEYMERFPSHSPAKTQYATVEAA
PDKMRSSIIGTALKALQPFTTDTIAKLTSHSSLDLNKLGFPSIIDGFSEPDSKFELNVQVKRETNIGTKY
DEVEGINIGVNEYGYWQYPFKTVLNIDDRIEIVETDEFNEEVKYYFYVTSIDKKGQVTFEAKGDIYSSNV
TIRKFNSFAKPIAIFMVVPDYNSANHGIASILVNQIVFELSKNSQLYTEKQSTHRRVIFHLDEAGNMPQI
PNLSQKVNVSLSRGLRFNFFVQAFSQIKDTYGDAYDAIMDACQNKVFIMATQEQTLKDFSDMIGSQQITV
RSRSGETDSLKSSITENQEERPLLRADELAHLQEGETVVVRNLKRQDNKRRKVMSYPIFNSGKYAMKYRY
QYLADLMDTGKSIIHFRPLLKERCEHRHLQLETTLVDWEKIIENMQAGIENDSNNDIDDLTQSLNKSMGE
KANANNPEVYKEENNKKPVTRQKKVLTLKAQLSAEEFDRLTRRFTKTIRMYEKDGNNVDRLTLYKLKQYL
KLRVRNNDITEEQKNKSINYVKQLIEEAKAK

Protein domains

Predicted by InterproScan.

• TraM recognition site of TraD and TraG (TraG-D_C) (616-733)

• Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf) (190-459)

Protein structure

No available structure.

Host bacterium

• ID: 16494
• GenBank: NZ_CP042047
• Plasmid name: pSALNBL75
• Incompatibility group: -
• Plasmid size: 75938 bp
• Coordinate of oriT [Strand]: 19071..19246 [-]
• Host baterium: Staphylococcus aureus strain B2-7A

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: AcrIIA21