Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   115985
Name   oriT_223|p223B in_silico
Organism   Lactococcus lactis subsp. lactis strain 223
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP135056 (21453..21589 [+], 137 nt)
oriT length   137 nt
IRs (inverted repeats)      21..28, 42..49  (AAAAGGGG..CCCCTTTT)
 25..30, 39..44  (GGGGAA..TTCCCC)
Location of nic site      104..105
Conserved sequence flanking the
  nic site    		  GCTTGCAGTA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 137 nt

>oriT_223|p223B
CGACACAATCCAAAGGGGATAAAAGGGGAAAGTAAAACTTCCCCCTTTTCAAGCCACATTGTAATACAAGAACGAAGTGATTTGTATTACAATGTGATAGCTTGCAGTATTTATGGTTTTATATGGTCTATTTTGTT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   10222 GenBank   WP_101945462
Name   Relaxase_LL223_RS13175_223|p223B insolico UniProt ID   _
Length   410 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 410 a.a.        Molecular weight: 47775.34 Da        Isoelectric Point: 7.3196

>WP_101945462.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Lactococcus]
MATIAKISNGASAASALNYALGQDRPMHEKTEQWLQDHQLERPVELTNCRAVAVGGTNGIDPFIVKEQFD
VIRQLHNQTKESNQVLRITQSFALDELNPKVQKDWQKANDLGVELAENLYPNHQSAVYTHLDGKNHVLHN
HIIVNKVNLETGKKLREQKGESVQRAREMNDRLASRENWHILEPPKERQTETEKELIAKNEYSYMDDLRE
RINKSLQDVSVSSYETFKERLSDNGVILSERDQTFSYAFLDANNKQRRARETRLGSDFGRETILHELENR
ARQNEFSAVEQREPAITPLERDTQQRESEIVSLEQAIEPRKSEALKRESTINRFIDTIKQFAGRVPELTQ
RVTRKLKQTKEKILDDFERRFSKDMKNYEQEQQKSLEKQANRDVQSEKKPTKDHDRGMSR

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(9-280)


  Protein structure



No available structure.




Host bacterium

ID   16418 GenBank   NZ_CP135056
Plasmid name   223|p223B Incompatibility group   -
Plasmid size   27448 bp Coordinate of oriT [Strand]   21453..21589 [+]
Host baterium   Lactococcus lactis subsp. lactis strain 223

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -