Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   115979
Name   oriT_IMT49658-1|unnamed1 in_silico
Organism   Enterobacter hormaechei strain IMT49658-1
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP135271 (92478..92732 [-], 255 nt)
oriT length   255 nt
IRs (inverted repeats)      153..158, 160..165  (AAAAGT..ACTTTT)
 122..130, 135..143  (TTAAGGCTT..AAGCCTTAA)
 49..55, 60..66  (GAATTTT..AAAATTC)
Location of nic site      78..79
Conserved sequence flanking the
  nic site    		  TTTGGTTAAA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 255 nt

>oriT_IMT49658-1|unnamed1
GGATTTAGGTTTTTTTTTAATCGCTTCACATTTCGTTAGCATGCGCAGGAATTTTTGATAAAATTCTGGTTAGTTTGGTTAAAAAGTGTTACAAGTAAGCGGTGTGGTTGAAGGGATAGATTTAAGGCTTATTCAAGCCTTAAGAAAATACTAAAAGTTACTTTTCACCCTACCGAACACCTAACAAAAAATCCATGTTGAAGATTTGAACAATTGTAATGGCGCAAGGACAATCAGCACATGTCAGAATCTGAT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   10219 GenBank   WP_012477394
Name   mobH_RP424_RS23050_IMT49658-1|unnamed1 insolico UniProt ID   A0A2Z2E7B6
Length   1050 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1050 a.a.        Molecular weight: 118067.47 Da        Isoelectric Point: 4.6347

>WP_012477394.1 MULTISPECIES: MobH family relaxase [Enterobacterales]
MMMNFRALYLCIKRILGIFSSQENDATSVMIEDISSLSPFAQILGDQKYTVPDHPNPEVLKFIEYPTRPT
GIQTFNEQSILSLYREKLHSISMMLAISDSDIRDDAYTFTNLVLKPLVEYVRWIHLLPASENHHHNGIGG
LLSHSLEVAILSLKNAHHSELRPIGYQDEEVVRRKVYLYAAFICGLVHDAGKVYDLDIVSLNLASPIIWT
PSSQSLLDWARENDVVEYEIHWRKRIHNQHNIWSSVFLERILNPVCLAFLDRVNKERVYSKMITALNVYT
DGNDFLSKCVRTADFYSTGTDLNVLRDPIMGLRSNDAAARAISTIKHNFTSININNYNAKPMHIIIVNGE
VYLNENAFLDFVLNDFELHKYNFPQGEAGKTVLVESLVQRGYVEPYDDERVVHYFIPGIYSENEISNIFR
NGIGKLEFYNLLKLRWIGLIFDSYKIPDSVPGLFSVNANKDFIYIDEQKTVTEYRRPVPGRDVITKITDT
VETAVLKVNDLGRSSASIDVDIHSKKNEGSSDDFEKKAESDNEIDNDTQIVKSEGEEAADPVIPDIEESE
DESAKDTESHVLVNQLHELLLSAPLSNDYIVCVDAVPYLNIDTTMALLPGLDEKAFSEEPYFQLTFREGS
LDGMWIVRDIDDLRLVQLGDNCAGFQLTYHEPRRPTTLKSLFNTSMYQALVINDESSVENSAPRPKQTLE
LPPPRVNAVEEHSGDVEYHGTDSASATGPLKTEAVEYEHYQHLFEKEDEEHEIIDYTDFSQLSVSRPEVG
SCATSSSVHNEKLLSEPSELPELNREQNADPQGTNERSMDVSVGQENSEPDTEGNCPPPAEVVYSQTEAA
ATSVMASEEPALPPVLEESNGEHAPTDAKGHHLSPALARLFAPTAPVEKQNPKRNRNKSSDKAEVQKPAS
PVSGHNLNSKVFASTESDQNGEFSLISEGDVTELEFVEIALVLHQILSKMEVAFKRKRKNRFMVSTPNTL
YLTQSCVEKFGSQLEAQDLFNKLPQYLVNSGAVINTKCHAFNMPTLLAASDRAKVDIERIINNLKEAGNL

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(108-304)


  Protein structure


Source ID Structure
AlphaFold DB A0A2Z2E7B6


T4CP

ID   11938 GenBank   WP_001284073
Name   traD_RP424_RS23055_IMT49658-1|unnamed1 insolico UniProt ID   _
Length   694 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 694 a.a.        Molecular weight: 78123.91 Da        Isoelectric Point: 8.0177

>WP_001284073.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Enterobacterales]
MSDSKRTNLHAQENFYRPILEYRSASILLICSVSMLYMGLSSDGLDIAPIVLFTSILLFLLCLYRCKTAA
PFLMAHWRVFKRHFMFVSLDSLRVINKSNFFSNERKYRQLVQDYQNKNKDIPERKSYFCDGFEWGPEHAD
RAYQIANLSSDKREIELPFVFNPIKRHFDAMARKMGGSNAIFAVERREPIFVTEDNWFGHTLITGNVGTG
KTVLQRLLSISMLHLGHVVVVIDPKNDAEWRESLMEEAKTLGLPFYKFHPGQPASSVCIDVCNTYTNVSD
LTSRLLSLVTVPGEVNPFVQYAKALVSNVISGLSYIEKKPSIYLIHKNMKSHMSIVNLTVKVMESCYARY
YGYDVWTEKVKYVANDTLPVRFKRLAEWFTAHFMNYEGSEQIDWLDTVSQLIDYSMSDPEHMAKMTAGIM
PVFDMLIEKPLNELLSPNPNSVSSREIVTSEGMFSTGGVLYISLDGLSNPDTAAAISQLIMSDLTSCAGS
RYNAQDGDMSANSRISIFVDEAHSAINNPMINLLAQGRAAKIALFICTQTISDFIAAASVETANRITGLC
NNYISLRVNDTPTQTLVVENFGKSAISTNMVTYTTGSETSLPHNNFSGSISERKQTTLEESIPKDLLGQV
PMFHIVARLQDGRKVVGQIPIAVAEKQMKPNTTLSEMLFKKAGKVTLRQNLDIKNLNKFLRKLH

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(406-609)

AAA-like domain (AAA_10)

(199-249)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 218044..227284

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RP424_RS23755 213187..213903 - 717 WP_000838219 zinc finger-like domain-containing protein -
RP424_RS23760 213900..214100 - 201 WP_000594930 hypothetical protein -
RP424_RS23765 214120..215172 - 1053 WP_001065778 hypothetical protein -
RP424_RS23770 215649..216524 + 876 WP_000594611 RepB family plasmid replication initiator protein -
RP424_RS23775 217673..218026 + 354 WP_000423602 hypothetical protein -
RP424_RS23780 218044..218394 + 351 WP_001447770 type IV conjugative transfer system protein TraL traL
RP424_RS23785 218406..219194 + 789 WP_000783153 TraE/TraK family type IV conjugative transfer system protein traE
RP424_RS23790 219194..220465 + 1272 WP_000592092 type-F conjugative transfer system secretin TraK traK
RP424_RS23795 220467..220928 + 462 WP_000521242 plasmid transfer protein HtdO -
RP424_RS23800 220918..222273 + 1356 WP_000351841 IncHI-type conjugal transfer protein TrhB traB
RP424_RS23805 222281..222763 + 483 WP_000377632 hypothetical protein -
RP424_RS23810 222882..223634 + 753 WP_182911723 protein-disulfide isomerase HtdT -
RP424_RS23815 223644..224594 + 951 WP_219715965 IncHI-type conjugal transfer lipoprotein TrhV traV
RP424_RS23820 224603..227284 + 2682 WP_000387412 TraC family protein virb4
RP424_RS23825 229833..229994 + 162 WP_001371949 hypothetical protein -

Host bacterium

ID   16412 GenBank   NZ_CP135271
Plasmid name   IMT49658-1|unnamed1 Incompatibility group   IncHI2
Plasmid size   294358 bp Coordinate of oriT [Strand]   92478..92732 [-]
Host baterium   Enterobacter hormaechei strain IMT49658-1

Cargo genes

Drug resistance gene   aadA5, qacE, sul1, armA, blaTEM-1B, blaCTX-M-3, tet(D), catA2, blaDHA-1, qnrB4, ere(A), aac(6')-IIc
Virulence gene   -
Metal resistance gene   terD, terE, merE, merD, merA, merC, merP, merT, merR, terW, terZ, terA, terB, terC
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -