Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   115954
Name   oriT_pOHT403-4_3 in_silico
Organism   Enterobacter hormaechei strain OHT403-4
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP135279 (27399..27480 [-], 82 nt)
oriT length   82 nt
IRs (inverted repeats)      27..32, 35..40  (GCCACC..GGTGGC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 82 nt

>oriT_pOHT403-4_3
CGGGACAGGATATGTTTTGTATTACCGCCACCACGGTGGCAGCAATCCAAAACTCCTGGTCAGGGCGAAGCCCCGACACCCC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   10203 GenBank   WP_032440569
Name   traI_RRJ53_RS24560_pOHT403-4_3 insolico UniProt ID   A0A7G3L4G1
Length   642 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 642 a.a.        Molecular weight: 74384.24 Da        Isoelectric Point: 8.3203

>WP_032440569.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MNAIIPPKRRDRKSSFVQLVAYVSVRDDVPLKDELKEDERFQRPSRSRQAIFDRLVDYMNRSEGTIIEQI
SDMTPEGDIRCSVDGVICQHNLMSVETAAMEMNSIAMQNTHVKDAVYHYILSWQEDENPSDDQVFDSVKH
SLKRLGMEEHQYVAAIHRDTDNLHVHIAANRVHPVSYRAANVWNDADKLQRTCRELELKHGFKVDNGSWV
RDADNNIVRARKGYRNAPRSARQLEHFSDKESLHHFAVQHVRKPVNAMFREKNASWEKLHQVMNDAGLML
ETSGKGLIVRDVCGDEKLAVKSSRVHPGMTLSRLEPALGTFVPSPRSAEMVKQSVQTSYSDKLHVRDRGA
RAERRQARADARIDLRERYEKYRNAWKKPDLHAAERFRAVSAAFKEQKTHIRDNEKDAHMRKLMYHIVEF
EREKSMAALRIALKAERQKLYDEGMMRPLTYRAWTEQQALHGDQAALSQLRGWSYRQNRKNRTPQASDNI
IYCAPADDTSLLKADGYEARLHRDGAVVYSRQGQDAIIDRGDRVEVINPYDDGDLNTRIAVDMVAWKSGE
RVEFSRDGSFAYAAGDAVAAHNLHHRDSIIVPSDREQYDYTREKFNQLKGRYTDSEREALRQQYDDRYYD
DVRHNDDSSYKP

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(52-307)


  Protein structure


Source ID Structure
AlphaFold DB A0A7G3L4G1


T4CP

ID   11913 GenBank   WP_032440593
Name   trbC_RRJ53_RS24765_pOHT403-4_3 insolico UniProt ID   _
Length   730 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 730 a.a.        Molecular weight: 82679.78 Da        Isoelectric Point: 6.3201

>WP_032440593.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacterales]
MQQKQKPVDAARLQRQVHQSMVEEMAYRTGPLLLAIIAIFGAAIVWPVLLWAAIPVMLVWVPMFMLNEFR
MPARMPMDMDRLDPSTETMRPGKLLGFLPVTKLNRHMEKAAGVFYTGYERTLDGGREIWLRMDDLTRHLI
LMATTGAGKTEMLLGFVLNALCWAKGLIFSDGKAQNDVWFAVASLSRRFGREDDLRLLNYITGGQSRSQR
LLENDKSRQQSNNTNPFALAQETYITQLMDSMMPEAGNDRTWQEKAKGMNQVLTKALIYKSRKEQKVMSQ
RLIQEYLSLDKFAELYLQAEAEEWHEEIRTGLRNYLSTSVPGFDMSLITKPSEWSQEARNQHGYLTSQYN
RMLSLFSDTYGHVFSSGAGDIDLRDVVHNDRILVILIPALELSASEALTLGRLTTSQLAMILSQDLGERM
EGKAEDILVIKKFRDRFPFLWLADEVGSYYSDTIGQLATQVRSLGYALVLAGQDLQKLKTAVGDKLWTLL
GNMFTRIFGTIIDPTETAEFAAKSAGMEYQAIQDSMEYRAGAIGGSYADTGRVTVQEKPKLPFDELQALN
QGEQATVFKGAVIRNNALYIADDDKITDKDVRINRFVEVEWPTYDSLSHLLPAKVRRDRPRPASINRIMA
IVKNDKIRHAPDSMIIQDGVLYAIADEAMYFDEVAPTPPNTVQRATQLLSLAAAVLTETRGRYRVEYPEP
EKLSVKKDELEKYQHIHQSAVTQQADGFSY

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(439-560)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 2432..18290

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RRJ53_RS24445 (RRJ53_24445) 1854..2435 - 582 WP_050597335 conjugal transfer protein TraX -
RRJ53_RS24450 (RRJ53_24450) 2432..3628 - 1197 WP_032440590 conjugal transfer protein TraW traW
RRJ53_RS24455 (RRJ53_24455) 3638..6685 - 3048 WP_032440589 conjugal transfer protein traU
RRJ53_RS24460 (RRJ53_24460) 6695..7258 - 564 WP_045325070 hypothetical protein traT
RRJ53_RS24465 (RRJ53_24465) 7302..7703 - 402 WP_032440588 DUF6750 family protein traR
RRJ53_RS24470 (RRJ53_24470) 7759..8292 - 534 WP_032440587 conjugal transfer protein TraQ traQ
RRJ53_RS24475 (RRJ53_24475) 8302..9021 - 720 WP_050597333 conjugal transfer protein TraP traP
RRJ53_RS24480 (RRJ53_24480) 9028..10368 - 1341 WP_032440586 conjugal transfer protein TraO traO
RRJ53_RS24485 (RRJ53_24485) 10372..11475 - 1104 WP_077264204 DotH/IcmK family type IV secretion protein traN
RRJ53_RS24490 (RRJ53_24490) 11486..12154 - 669 WP_032440585 DotI/IcmL/TraM family protein traM
RRJ53_RS24495 (RRJ53_24495) 12226..12579 - 354 WP_032440584 hypothetical protein traL
RRJ53_RS24500 (RRJ53_24500) 12613..16026 - 3414 WP_052145516 LPD7 domain-containing protein -
RRJ53_RS24505 (RRJ53_24505) 16044..16310 - 267 WP_032440577 IcmT/TraK family protein traK
RRJ53_RS24510 (RRJ53_24510) 16307..17494 - 1188 WP_077255878 plasmid transfer ATPase TraJ virB11
RRJ53_RS24515 (RRJ53_24515) 17472..18290 - 819 WP_315434907 type IV secretory system conjugative DNA transfer family protein traI
RRJ53_RS24520 (RRJ53_24520) 18287..18751 - 465 WP_225546581 DotD/TraH family lipoprotein -
RRJ53_RS24525 (RRJ53_24525) 18777..19256 - 480 WP_032440574 lytic transglycosylase domain-containing protein -
RRJ53_RS24530 (RRJ53_24530) 19269..19637 - 369 WP_126523558 hypothetical protein -
RRJ53_RS24535 (RRJ53_24535) 19988..20215 - 228 WP_032440573 hypothetical protein -
RRJ53_RS24540 (RRJ53_24540) 20825..21667 + 843 WP_032440572 helix-turn-helix domain-containing protein -
RRJ53_RS24545 (RRJ53_24545) 21727..22656 + 930 WP_032440581 glycosyltransferase family 9 protein -
RRJ53_RS24550 (RRJ53_24550) 22649..23143 + 495 WP_032440571 molybdopterin-dependent oxidoreductase -

Host bacterium

ID   16387 GenBank   NZ_CP135279
Plasmid name   pOHT403-4_3 Incompatibility group   IncFII
Plasmid size   61235 bp Coordinate of oriT [Strand]   27399..27480 [-]
Host baterium   Enterobacter hormaechei strain OHT403-4

Cargo genes

Drug resistance gene   tet(A), qnrS1
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -