Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   115898
Name   oriT_pEh7_3 in_silico
Organism   Enterobacter hormaechei strain Ehh_7
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP126853 (46214..46300 [-], 87 nt)
oriT length   87 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 87 nt

>oriT_pEh7_3
GGGTGTCGGGGCGGAGCCCTGACCAGGTGGCATTTGTGATATCGTGCGTGCGCGGTATTACAAATGCACATCCTGTCCCGTCTTTCG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   10179 GenBank   WP_022650008
Name   Relaxase_QPR83_RS24660_pEh7_3 insolico UniProt ID   A0A4Q2QTK9
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 102314.37 Da        Isoelectric Point: 8.2756

>WP_022650008.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Enterobacterales]
MIPVIPPKRQDGKSSFGDLVSYVSVRDEPRDDDLMEAVRASGTKPEMPHSSRFSRLVDYATKLRDESFIS
LVDVMPDGGEWVNFYGVTCFHNCTSIESAAEEMEFSARKTKFAHNKSDPVFHYILSWQSHESPRPEQIYD
SVSHTLSRLGLAGHQFVAAVHTDTDNLHVHIAVNRVHPETGYLNRLAYSQEKLSKACRELELKHGFSPDN
GCYVIAPDNRIVRRTSVERDRQSAWKRGRNQSLREYIADTAIAGLRESPVTDWSSLHQRFAKEGLFLTKE
SGELKVKDGWDSERPGVTLSAFGHAFDTDKLIRKLGEFVPPVQDIFSQVPAVGRYEPDKIIMPSRPERMT
EKESLSDYAIARLRAPLIALDQDPTQRTIQSVHTLLAQSGLYLKEQHDRLVICDGYDSTRTPVRAERVWP
ALTKAILDSYKGGWQPVPKDIFQQVPPAEQFTGGSLEATPVSDREWRKLRTGSGPQGALKREIFSDKESL
WGYAVSHCRQDIESLIGSGEFTWERCHELLARQGLLLMREQQGLVVMDAYNHEQTPVKASHVHPDLTLAR
AEPHAGSFVSVPADIFERVKPASRYNPELAVSDRDIPGMKRDPELRRQRREARAAAREDLKARYAAWRAN
WQRPDLHGRERYQAIHDECRIRKARIRIEHRDPLVRKLHYHIAELQRMQALIALKETMKAERAELIGQGK
WYPPSYRQWVEAEAVKGDKAAISQLRGWDYRDRRGNAVKSTTDNRCVVICEPGGTPMYSGVPGLRASLKK
NGRVQFRDPETGRHICTDYGDRVIFRNSGDYDALKRDMVKVAPVLFSRSPSVAMAPEGNNEQFNTAFAKM
VAWHNVNHRDEGEYQISRPEVDQVRVESEMRFAEMTNQGSNTEAVRNESGPNWKPPSPL

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(51-317)


  Protein structure


Source ID Structure
AlphaFold DB A0A4Q2QTK9


T4CP

ID   11891 GenBank   WP_022650006
Name   trbC_QPR83_RS24675_pEh7_3 insolico UniProt ID   _
Length   766 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 766 a.a.        Molecular weight: 87660.94 Da        Isoelectric Point: 6.8660

>WP_022650006.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacterales]
MNYTPVNQALMKRNAWNSPVLELLRDHLLYAGIMAGSVVAGFIWPLAIPLLLLLAIVASISFGTHRWRMP
MRMPVHLNKVDPSEDRKVRRSLFRRFPSLFQYETTQESKGKGIFYLGYRRMNDIGRELWLTIDDLTRHVM
FFASTGGGKTETIYAWMLNSFCWGRGFTFVDGKAQNDTARTCYYLARRFGREDDVEYINFMNSGMSRSEM
IHNGDKSRPQSHQWNPFCYSTEAFVAETMQSMLPTNVQGGEWQSRAIAMNKALVFGTKFYCVRENKTMSL
QLLREFMPLEKLAELYCRAIDDQWPEEAVSPLYNYLVDVPGFDMATVRQPSAWTEEPRKQHSYLTGQFLE
TFNTFTETFGNIFAEDAGDIDIRDSIHSDRILLVLIPAMNTSQHTTSALGRMFVTQQSMILARDLGHRLE
GLDSEALEITKYKGPFPYMNYLDEVGAYYTERIAVQATQVRSLDFSIVMMSQDQERIESQTSATNVATLM
QNAGTKVAGKIVSDDKTAKTITNAAGQEARANMVSLQRRDGIIGTSWIDGDHINIQMENKINVQDLIKLQ
PGENYTVFQGDPVPGASFYIEPQDKTCNAPVIINRYITINPPNLKQLRKLVPRTAQRRLPAPENVSKIIG
VLTEKPSRRRKAAKAEPWKIIDTFQQRLANRQEAHNLMTEYDTDHRGREENLWEEALEVIRTTTMAERTI
RYITLNKPESEPTQVSAEEIQLAPDAILRTLTLPQPAYIPPSRYRNEPFISPKLPDSPHPDMEWPE

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(440-528)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 52423..86867

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QPR83_RS24665 (QPR83_24650) 49439..49642 - 204 WP_123839396 hypothetical protein -
QPR83_RS24670 (QPR83_24655) 49776..50045 - 270 WP_022650007 hypothetical protein -
QPR83_RS24675 (QPR83_24660) 50119..52419 - 2301 WP_022650006 F-type conjugative transfer protein TrbC -
QPR83_RS24680 (QPR83_24665) 52423..53679 - 1257 WP_126530067 hypothetical protein trbB
QPR83_RS24685 (QPR83_24670) 53660..54868 - 1209 WP_022650004 hypothetical protein trbA
QPR83_RS24690 (QPR83_24675) 55189..55821 - 633 WP_022650003 plasmid IncI1-type surface exclusion protein ExcA -
QPR83_RS24695 (QPR83_24680) 55883..58042 - 2160 WP_022650002 DotA/TraY family protein traY
QPR83_RS24700 (QPR83_24685) 58100..58642 - 543 WP_022650001 conjugal transfer protein TraX -
QPR83_RS24705 (QPR83_24690) 58639..59841 - 1203 WP_022650000 conjugal transfer protein TraW traW
QPR83_RS24710 (QPR83_24695) 59854..60423 - 570 WP_022649999 hypothetical protein traV
QPR83_RS24715 (QPR83_24700) 60423..63476 - 3054 WP_022649998 hypothetical protein traU
QPR83_RS24720 (QPR83_24705) 63563..64288 - 726 WP_063864836 hypothetical protein traT
QPR83_RS24725 (QPR83_24710) 64472..64876 - 405 WP_022649996 DUF6750 family protein traR
QPR83_RS24730 (QPR83_24715) 64911..65435 - 525 WP_022649995 conjugal transfer protein TraQ traQ
QPR83_RS24735 (QPR83_24720) 65435..66208 - 774 WP_123839398 conjugal transfer protein TraP traP
QPR83_RS24740 (QPR83_24725) 66189..67496 - 1308 WP_022649993 conjugal transfer protein TraO traO
QPR83_RS24745 (QPR83_24730) 67496..68476 - 981 WP_022649992 DotH/IcmK family type IV secretion protein traN
QPR83_RS24750 (QPR83_24735) 68487..69104 - 618 WP_179297403 DotI/IcmL family type IV secretion protein traM
QPR83_RS24755 (QPR83_24740) 69194..69544 - 351 WP_022649990 hypothetical protein traL
QPR83_RS24760 (QPR83_24745) 69562..73104 - 3543 WP_032635082 LPD7 domain-containing protein -
QPR83_RS24765 (QPR83_24750) 73176..73463 - 288 WP_022649988 hypothetical protein traK
QPR83_RS24770 (QPR83_24755) 73460..74620 - 1161 WP_022649987 plasmid transfer ATPase TraJ virB11
QPR83_RS24775 (QPR83_24760) 74633..75439 - 807 WP_022649986 type IV secretory system conjugative DNA transfer family protein traI
QPR83_RS24780 (QPR83_24765) 75436..75909 - 474 WP_022649985 DotD/TraH family lipoprotein -
QPR83_RS24785 (QPR83_24770) 75980..76396 - 417 WP_123839394 hypothetical protein -
QPR83_RS24790 (QPR83_24775) 76505..77710 - 1206 WP_062939536 conjugal transfer protein TraF -
QPR83_RS24795 (QPR83_24780) 77816..78652 - 837 WP_022649983 hypothetical protein traE
QPR83_RS24800 (QPR83_24785) 78799..79275 - 477 WP_022649982 hypothetical protein -
QPR83_RS24805 (QPR83_24790) 79295..80455 - 1161 WP_022649981 site-specific integrase -
QPR83_RS24810 (QPR83_24795) 81036..82370 - 1335 WP_032627586 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
QPR83_RS24815 (QPR83_24800) 82367..83041 - 675 WP_022649979 prepilin peptidase -
QPR83_RS24820 (QPR83_24805) 83026..83508 - 483 WP_045261878 lytic transglycosylase domain-containing protein virB1
QPR83_RS24825 (QPR83_24810) 83593..84207 - 615 WP_022649977 type 4 pilus major pilin -
QPR83_RS24830 (QPR83_24815) 84221..85315 - 1095 WP_032623575 type II secretion system F family protein -
QPR83_RS24835 (QPR83_24820) 85308..86867 - 1560 WP_022649975 GspE/PulE family protein virB11
QPR83_RS24840 (QPR83_24825) 86870..87385 - 516 WP_022649974 type IV pilus biogenesis protein PilP -
QPR83_RS24845 (QPR83_24830) 87369..88673 - 1305 WP_022649973 type 4b pilus protein PilO2 -
QPR83_RS24850 (QPR83_24835) 88675..90351 - 1677 WP_022649972 PilN family type IVB pilus formation outer membrane protein -
QPR83_RS24855 (QPR83_24840) 90366..90803 - 438 WP_022649971 type IV pilus biogenesis protein PilM -

Host bacterium

ID   16331 GenBank   NZ_CP126853
Plasmid name   pEh7_3 Incompatibility group   -
Plasmid size   97002 bp Coordinate of oriT [Strand]   46214..46300 [-]
Host baterium   Enterobacter hormaechei strain Ehh_7

Cargo genes

Drug resistance gene   blaLAP-2, qnrS1
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIC1, AcrIIA7