Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 115895
• Name: oriT_7.8.2|unnamed2
• Organism: Lactiplantibacillus pentosus strain 7.8.2
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP134790 (2687..2707 [-], 21 nt)
• oriT length: 21 nt
• IRs (inverted repeats): _
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 21 nt

>oriT_7.8.2|unnamed2
AGTGGGGTGTAAGTGCGCATT

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobQ_RNT80_RS16765_7.8.2|unnamed2

• ID: 10178
• GenBank: WP_216804304
• Name: mobQ_RNT80_RS16765_7.8.2|unnamed2
• UniProt ID: _
• Length: 687 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 687 a.a.
• Molecular weight: 80482.21 Da
• Isoelectric Point: 9.7777

>WP_216804304.1 MobQ family relaxase [Lactiplantibacillus pentosus]
MAIFHMSFSNISAGKGRSAIASAAYRSGEKLFDDQEGRHYFYARSVMPESFILTPKNAPAWASDREKLWN
EVERKDRRANSRYAKEFNVALPVELSEDEQKELLTKYVQENFVDQGMVADVAIHRDHQDNPHAHVMLTNR
PFNSDGTWGIKSKKQYILDDNGNKTYTGTSKYPKSRKILMVDWDKKEKITEWRHNWAASVNQALEQKNIP
DRISEKSFVEQGIADTPMQHEGINSKRSKRKEFNQQVKKYRKTKANYKNNQEKVINRGHLDSLSKHFSFN
EKKVVKELSHELKTYISLESLDDKRRMLFNWKNSTLIKHAVGEDITKQLLTINQQESSLKKADELLNKVV
DRTTKKLYPELDFEQTTAAERRELIKETNSEQTIFKGSELNERLMNIRDDLLVRQLLTFTKRPYVGWKLL
MQQEKEVKIELKYTLMIHDDSLESLEHVDQGLLEKYSPTEQQKITRAVKDLRTITAVKQVIQTQYQEVLR
RAFPNGNFNELPMIKLEQAYTAVMYYDPALKPCKVETIAQWQKNPPRVFNTQEHQQGLSYLSGQLSLDQL
ENHHLQRVLKHDGTKQLFFGECKADPTIKNSQIEKIQKQLKGQQAKDDQYRKVNIGHYQPLNYKPVSPSY
YLKTAFSNAIMTALYARDEDYERQKQAQGLKETEWEMTKKQRQHQTRNRHEDGGMHL

Protein domains

Predicted by InterproScan.

• Nicking enzyme C-terminal middle helical domain (NES_C_h) (276-384)

• MobA/MobL family (MobA_MobL) (17-241)

Protein structure

No available structure.

Host bacterium

• ID: 16328
• GenBank: NZ_CP134790
• Plasmid name: 7.8.2|unnamed2
• Incompatibility group: -
• Plasmid size: 53893 bp
• Coordinate of oriT [Strand]: 2687..2707 [-]
• Host baterium: Lactiplantibacillus pentosus strain 7.8.2

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: AcrIIA21