Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   115875
Name   oriT_3.2.17|unnamed in_silico
Organism   Enterobacter cancerogenus strain 3.2.17
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP134405 (74637..74749 [+], 113 nt)
oriT length   113 nt
IRs (inverted repeats)      93..99, 103..109  (TAATTTA..TAAATTA)
 90..95, 97..102  (AAATAA..TTATTT)
 29..34, 41..46  (GCAAAA..TTTTGC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 113 nt

>oriT_3.2.17|unnamed
TTCTCACCACAAAAGCACCACACCCCACGCAAAACGTACTTTTTGCTGATTGGCTATTCAGTTCAGTAAGTTAGCGTTTGAATAATGTGAAATAATTTATTTTAAATTACTAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   10165 GenBank   WP_313830629
Name   traI_RIN64_RS23120_3.2.17|unnamed insolico UniProt ID   _
Length   1747 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1747 a.a.        Molecular weight: 189258.36 Da        Isoelectric Point: 6.3948

>WP_313830629.1 conjugative transfer relaxase/helicase TraI [Enterobacter cancerogenus]
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMGERWAGEGAKALGLSGSVDVKTFTRVLEGKLPDGSDLSRAQ
DGTNKHRPGYDLTFSAPKSISVMAMLGGDTRLIDAHNRAVDTAIKQIEALASTRVMTEGKSETQLTGNLV
MALFNHDTSRDQEPQLHTHAVVANVTQHDGTWRTLSSDKIGKTGFIENVYANQIAFGRLYRAALKDDVTA
MGYETETVGKHGMWELKGVPTEPYSSRSKAIREAVGDDASLKSRDVAALDTRQSKQKVDPEQRMAEWMAT
LKETGFDIQAYREAADQRARGGDIPVTTPEPVNVDTAVGQSIAMLSDRRARFTYSELLATTLGQLPARPG
MIEQAREGIDAAIKNEQLIPLDKEKGLFTSNVHVLDELSVSALSLELQQQGRVDVFPDKSVPRSRAFGDA
VSVLAQDRSPVAIISGQGGAAGQRERVAELAMMAREQGRDVQIMAADRRSAANLARDERLSGELITDRRG
LTEGMTFIPGSTLIVDQGEKLTLKETLTLLDGALRHNVQLLIADSGQRTGTGSALTVMKEAGVSTLAWQG
GEKTRVSVISEPDRRERYDRLAADFAKSIRAGEEAVVQISGAREQAALAATVRDALKGEKVLGERDISIT
TLEPVWLDGRHRQVRDHYREGMVMERWNAEERSRERFVIDRVTSRNNSLTLRNAQGETLVTRLTELDSSW
SLYRTGTLQVTEGDRLAVLGQTQGARLKGGDSVTVTGIDEKGIHVSLPGRKTQAVLPAGDSPFTAPKIGQ
GWVESPGRSVSDSATVYASLTQREMDNATLNGLARSGSEIRLYSAQTVQKTEEKLSRQSAWTVVTAQVKE
AAGKDSLGEALAHQKAALHTPEQQAIHLAIPSLEGNGLAFTRPQLMAAAKDFAQDKLSLAAIEDEIARQT
RSGALISVPVSQGNGLQQLVSRQSYNAEKSIIRHVLEGKDAITPLMGKVPDAQLAGLTDGQQNATRMILE
SPDRFTLVQGYAGVGKTTQFRAVMSAIGTLPEEQRPRVIGVAPTHRAVGEMRDAGVPESQTLAAFIHDTQ
QQLRGGERPDFSNVLFLIDESSMVGNADMAKAYSLIAGGGGRAVSSGDTDQLQSIAPGQPFRLLQKRSAI
DTVVMQEIVRQTPALRPAVYSLIERNIGSALTTLESVAPQQVPRRPDAWQPDGSVMEFSREQEKAIADAI
KAGDLAPGGQPATLLEAIVKDYTGRTPQAQAQTIVITALNADRRQLNAMIHDARREAGELGEKEAIVPVL
TPANIRDGELRRMDTWQTHASSKVLLDNTYYRIDALDKDAHLVTLKDEQGNARSLSPAQAATEGVTLYRQ
DTIAVSPGDRMRFSKSDNDRGFVANSVWTVSDIKGDSVTLTDGKQTRTVRPGVEPAEQHIDLAYAVTVYG
AQGASEPFSISLQGTDGGRKQMVNFESAYVALSRMKQHAQVYTDNREKWVAAMEKSQAKSTAHDIVEPRN
DCAVANAARLTASAKGLGEVPAGRAALRQAGLRPETTLAKFISPGRKYPQPHVALPAFDRNGRQAGVWLS
TLTDGDGRLQGLSGERRVMGSGDAAFAGLQASRNGESLMAGDMTEGVRLARENPQSGVVVRIGDAEGRPW
NPGAITGGRVWADVVPDGTGTQHGEKIPPEVLAQQALEAQQRRELEKRAEDAVRELARGGEKSAEPADAV
RELARELGEGKNRERSTDVTLPESPDVRARDEAVSRVAHENVQRDRLQQMERDTVRVLEREKTQGGD

  Protein domains


Predicted by InterproScan.

AAA domain (AAA_30)

(967-1153)

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(573-625)

TrwC relaxase (TrwC)

(10-283)

DNA helicase TraI (TraI_C)

(1430-1551)

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(631-710)


  Protein structure



No available structure.




T4CP

ID   11875 GenBank   WP_313830627
Name   traD_RIN64_RS23115_3.2.17|unnamed insolico UniProt ID   _
Length   746 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 746 a.a.        Molecular weight: 84085.92 Da        Isoelectric Point: 5.0963

>WP_313830627.1 type IV conjugative transfer system coupling protein TraD [Enterobacter cancerogenus]
MSFNAKDMTQGGQIASMRIRMFSQIANIILYCLVILFGIIVALTLAVKLSWQTFVNGMAWYWCQTLAPMR
DIFRSQPVYTIRYYGKELKYNAQQLLQDKYVIWCADQVLTAFILAAIIAAVVCVIAFFVTTWILGHQGKQ
QSEDEVTGGRQLTDDPREVARMLKRDNLASDIKIGDLPLIKNSEIQNFCLHGTVGAGKSEVIRRLMNYAR
KRGDMVIVYDRSCEFVKSYYDPAIDKILNPMDARCAAWDLWRECLTQPDFDNVSNTLIPMGTKEDPFWQG
SGRTIFAEAAYLMRNDKDRSYSKLVDTLLSIKIEKLRSYLQNSPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEHNGEPFTIRDWMRGVREDGRNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVIGIQSYAQLEDIYGQKPAETLFDVLNTRAFFRSPSRNIA
EFAAGEIGEKEILKASEQYSYGVDPVRDGTSVGKDMERVTLVSYSDIQTLPDLSCYVTLPGPYPAVKMAL
KYQERPKVAPEFIQRRIDDKAEQRLNAALAAREAESRSVAMLFEPDTEVVAPVAGPENAVQPQQPQQPQQ
PQQPQQPQQPQQPQQPQQPQQPQPAPVITPRPAPVAASTSVAGTGGTESVLETKAEEAEQLPPGIDESGE
VVDMAAWEAWQAEQDELTPQERQRREEVNINVHRTHDDDLEPGGYI

  Protein domains


Predicted by InterproScan.

F sex factor protein N terminal (TraD_N)

(32-128)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(172-560)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 74095..97075

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RIN64_RS22960 (RIN64_22960) 69216..69395 + 180 Protein_79 theronine dehydrogenase -
RIN64_RS22965 (RIN64_22965) 69568..69717 + 150 Protein_80 DUF5431 family protein -
RIN64_RS22970 (RIN64_22970) 69665..69784 + 120 WP_272929840 type I toxin-antitoxin system Hok family toxin -
RIN64_RS22975 (RIN64_22975) 70487..70876 + 390 WP_313830589 ammonia monooxygenase -
RIN64_RS22980 (RIN64_22980) 70938..71219 + 282 WP_237658073 hypothetical protein -
RIN64_RS22985 (RIN64_22985) 71266..71622 + 357 WP_063449773 hypothetical protein -
RIN64_RS22990 (RIN64_22990) 71708..71971 + 264 WP_046622508 hypothetical protein -
RIN64_RS22995 (RIN64_22995) 72017..72847 + 831 WP_313830591 N-6 DNA methylase -
RIN64_RS23000 (RIN64_23000) 73525..74055 + 531 WP_313830593 antirestriction protein -
RIN64_RS23005 (RIN64_23005) 74095..74568 - 474 WP_313830595 transglycosylase SLT domain-containing protein virB1
RIN64_RS23010 (RIN64_23010) 74999..75394 + 396 WP_313830597 conjugal transfer relaxosome DNA-binding protein TraM -
RIN64_RS23015 (RIN64_23015) 75578..76282 + 705 WP_193037203 LuxR C-terminal-related transcriptional regulator -
RIN64_RS23020 (RIN64_23020) 76361..76585 + 225 WP_046622499 TraY domain-containing protein -
RIN64_RS23025 (RIN64_23025) 76632..76982 + 351 WP_202562312 type IV conjugative transfer system pilin TraA -
RIN64_RS23030 (RIN64_23030) 77119..77424 + 306 WP_063449767 type IV conjugative transfer system protein TraL traL
RIN64_RS23035 (RIN64_23035) 77439..78005 + 567 WP_046622494 type IV conjugative transfer system protein TraE traE
RIN64_RS23040 (RIN64_23040) 77992..78735 + 744 WP_313830604 type-F conjugative transfer system secretin TraK traK
RIN64_RS23045 (RIN64_23045) 78722..80125 + 1404 WP_313830606 F-type conjugal transfer pilus assembly protein TraB traB
RIN64_RS23050 (RIN64_23050) 80146..80682 + 537 WP_202562307 type IV conjugative transfer system lipoprotein TraV traV
RIN64_RS23055 (RIN64_23055) 80989..83619 + 2631 WP_313830609 type IV secretion system protein TraC virb4
RIN64_RS23060 (RIN64_23060) 83616..83963 + 348 WP_313830611 type-F conjugative transfer system protein TrbI -
RIN64_RS23065 (RIN64_23065) 83963..84610 + 648 WP_313830613 type-F conjugative transfer system protein TraW traW
RIN64_RS23070 (RIN64_23070) 84607..85593 + 987 WP_063449758 conjugal transfer pilus assembly protein TraU traU
RIN64_RS23075 (RIN64_23075) 85608..86231 + 624 WP_313830616 type-F conjugative transfer system pilin assembly protein TrbC trbC
RIN64_RS23080 (RIN64_23080) 86228..88114 + 1887 WP_313830618 type-F conjugative transfer system mating-pair stabilization protein TraN traN
RIN64_RS23085 (RIN64_23085) 88142..88879 + 738 WP_313830649 type-F conjugative transfer system pilin assembly protein TraF traF
RIN64_RS23090 (RIN64_23090) 88896..89141 + 246 WP_102750541 type-F conjugative transfer system pilin chaperone TraQ -
RIN64_RS23095 (RIN64_23095) 89131..89670 + 540 WP_313830621 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
RIN64_RS23100 (RIN64_23100) 89670..91043 + 1374 WP_313830651 conjugal transfer pilus assembly protein TraH traH
RIN64_RS23105 (RIN64_23105) 91043..93925 + 2883 WP_313830623 conjugal transfer mating-pair stabilization protein TraG traG
RIN64_RS23110 (RIN64_23110) 93937..94470 + 534 WP_313830625 hypothetical protein -
RIN64_RS23115 (RIN64_23115) 94835..97075 + 2241 WP_313830627 type IV conjugative transfer system coupling protein TraD virb4

Host bacterium

ID   16308 GenBank   NZ_CP134405
Plasmid name   3.2.17|unnamed Incompatibility group   IncFIB
Plasmid size   105076 bp Coordinate of oriT [Strand]   74637..74749 [+]
Host baterium   Enterobacter cancerogenus strain 3.2.17

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIA7