Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 115828 |
| Name | oriT_pEh24_OXA48  |
| Organism | Enterobacter hormaechei strain Ehh_24 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP126763 (43379..43484 [+], 106 nt) |
| oriT length | 106 nt |
| IRs (inverted repeats) | 30..35, 41..46 (CCGCCG..CGGCGG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 106 nt
>oriT_pEh24_OXA48
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 10141 | GenBank | WP_004187323 |
| Name | traI_QPR70_RS24240_pEh24_OXA48 |
UniProt ID | A0A3U8KUL3 |
| Length | 659 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75179.89 Da Isoelectric Point: 9.9948
>WP_004187323.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3U8KUL3 |
T4CP
| ID | 11848 | GenBank | WP_004206885 |
| Name | t4cp2_QPR70_RS23945_pEh24_OXA48 |
UniProt ID | _ |
| Length | 695 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 695 a.a. Molecular weight: 79538.91 Da Isoelectric Point: 4.8317
>WP_004206885.1 MULTISPECIES: conjugal transfer protein TrbC [Enterobacterales]
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 46280..62788
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| QPR70_RS24220 (QPR70_24185) | 41364..41675 | + | 312 | WP_004187333 | hypothetical protein | - |
| QPR70_RS24225 (QPR70_24190) | 41808..42344 | + | 537 | WP_004187332 | hypothetical protein | - |
| QPR70_RS24230 (QPR70_24195) | 42846..43241 | - | 396 | WP_019725163 | hypothetical protein | - |
| QPR70_RS24235 (QPR70_24200) | 43276..43803 | + | 528 | WP_172740549 | plasmid mobilization protein MobA | - |
| QPR70_RS24240 (QPR70_24205) | 43790..45769 | + | 1980 | WP_004187323 | TraI/MobA(P) family conjugative relaxase | - |
| QPR70_RS24245 (QPR70_24210) | 45783..46283 | + | 501 | WP_004187320 | DotD/TraH family lipoprotein | - |
| QPR70_RS24250 (QPR70_24215) | 46280..47059 | + | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| QPR70_RS24255 (QPR70_24220) | 47070..48233 | + | 1164 | WP_004187313 | plasmid transfer ATPase TraJ | virB11 |
| QPR70_RS24260 (QPR70_24225) | 48223..48483 | + | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| QPR70_RS24265 (QPR70_24230) | 48508..51945 | + | 3438 | WP_015586048 | LPD7 domain-containing protein | - |
| QPR70_RS24270 (QPR70_24235) | 51911..52423 | + | 513 | WP_011091071 | hypothetical protein | traL |
| QPR70_RS24275 (QPR70_24240) | 52424..52636 | - | 213 | WP_305953721 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| QPR70_RS24280 (QPR70_24245) | 52608..53018 | - | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| QPR70_RS24285 (QPR70_24250) | 53086..53799 | + | 714 | WP_004187467 | DotI/IcmL family type IV secretion protein | traM |
| QPR70_RS24290 (QPR70_24255) | 53808..54959 | + | 1152 | WP_004187471 | DotH/IcmK family type IV secretion protein | traN |
| QPR70_RS24295 (QPR70_24260) | 54971..56320 | + | 1350 | WP_004187474 | conjugal transfer protein TraO | traO |
| QPR70_RS24300 (QPR70_24265) | 56332..57036 | + | 705 | WP_015060002 | conjugal transfer protein TraP | traP |
| QPR70_RS24305 (QPR70_24270) | 57060..57590 | + | 531 | WP_004187478 | conjugal transfer protein TraQ | traQ |
| QPR70_RS24310 (QPR70_24275) | 57607..57996 | + | 390 | WP_004187479 | DUF6750 family protein | traR |
| QPR70_RS24315 (QPR70_24280) | 58042..58536 | + | 495 | WP_004187480 | hypothetical protein | - |
| QPR70_RS24320 (QPR70_24285) | 58533..61583 | + | 3051 | WP_004187482 | hypothetical protein | traU |
| QPR70_RS24325 (QPR70_24290) | 61580..62788 | + | 1209 | WP_011091082 | conjugal transfer protein TraW | traW |
| QPR70_RS24330 (QPR70_24295) | 62785..63381 | + | 597 | WP_032419542 | hypothetical protein | - |
| QPR70_RS24335 (QPR70_24305) | 63506..65568 | + | 2063 | Protein_81 | DotA/TraY family protein | - |
| QPR70_RS24340 (QPR70_24310) | 65570..66223 | + | 654 | WP_015060005 | hypothetical protein | - |
| QPR70_RS24345 (QPR70_24315) | 66304..66534 | + | 231 | WP_011091085 | IncL/M type plasmid replication protein RepC | - |
Host bacterium
| ID | 16261 | GenBank | NZ_CP126763 |
| Plasmid name | pEh24_OXA48 | Incompatibility group | IncL/M |
| Plasmid size | 66829 bp | Coordinate of oriT [Strand] | 43379..43484 [+] |
| Host baterium | Enterobacter hormaechei strain Ehh_24 |
Cargo genes
| Drug resistance gene | blaOXA-48 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |