Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 115820 |
| Name | oriT_pEh23_OXA48  |
| Organism | Enterobacter hormaechei strain Ehh_23 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP126769 (48771..48876 [+], 106 nt) |
| oriT length | 106 nt |
| IRs (inverted repeats) | 30..35, 41..46 (CCGCCG..CGGCGG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 106 nt
>oriT_pEh23_OXA48
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 10138 | GenBank | WP_004187323 |
| Name | traI_QPR74_RS24115_pEh23_OXA48 |
UniProt ID | A0A3U8KUL3 |
| Length | 659 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75179.89 Da Isoelectric Point: 9.9948
>WP_004187323.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3U8KUL3 |
T4CP
| ID | 11844 | GenBank | WP_004206885 |
| Name | t4cp2_QPR74_RS23800_pEh23_OXA48 |
UniProt ID | _ |
| Length | 695 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 695 a.a. Molecular weight: 79538.91 Da Isoelectric Point: 4.8317
>WP_004206885.1 MULTISPECIES: conjugal transfer protein TrbC [Enterobacterales]
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 51672..70961
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| QPR74_RS24095 (QPR74_24055) | 46757..47068 | + | 312 | WP_004187333 | hypothetical protein | - |
| QPR74_RS24100 (QPR74_24060) | 47201..47737 | + | 537 | WP_036970776 | hypothetical protein | - |
| QPR74_RS24105 (QPR74_24065) | 48238..48603 | - | 366 | WP_015586046 | hypothetical protein | - |
| QPR74_RS24110 (QPR74_24070) | 48878..49195 | + | 318 | WP_011091068 | plasmid mobilization protein MobA | - |
| QPR74_RS24115 (QPR74_24075) | 49182..51161 | + | 1980 | WP_004187323 | TraI/MobA(P) family conjugative relaxase | - |
| QPR74_RS24120 (QPR74_24080) | 51175..51675 | + | 501 | WP_004187320 | DotD/TraH family lipoprotein | - |
| QPR74_RS24125 (QPR74_24085) | 51672..52451 | + | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| QPR74_RS24130 (QPR74_24090) | 52462..53625 | + | 1164 | WP_004187313 | plasmid transfer ATPase TraJ | virB11 |
| QPR74_RS24135 (QPR74_24095) | 53615..53875 | + | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| QPR74_RS24140 (QPR74_24100) | 53900..57337 | + | 3438 | WP_015586048 | LPD7 domain-containing protein | - |
| QPR74_RS24145 (QPR74_24105) | 57303..57815 | + | 513 | WP_011091071 | hypothetical protein | traL |
| QPR74_RS24150 (QPR74_24110) | 57816..58028 | - | 213 | WP_305953721 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| QPR74_RS24155 (QPR74_24115) | 58000..58410 | - | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| QPR74_RS24160 (QPR74_24120) | 58478..59191 | + | 714 | WP_004187467 | DotI/IcmL family type IV secretion protein | traM |
| QPR74_RS24165 (QPR74_24125) | 59200..60351 | + | 1152 | WP_004187471 | DotH/IcmK family type IV secretion protein | traN |
| QPR74_RS24170 (QPR74_24130) | 60363..61712 | + | 1350 | WP_004187474 | conjugal transfer protein TraO | traO |
| QPR74_RS24175 (QPR74_24135) | 61724..62428 | + | 705 | WP_015060002 | conjugal transfer protein TraP | traP |
| QPR74_RS24180 (QPR74_24140) | 62452..62982 | + | 531 | WP_004187478 | conjugal transfer protein TraQ | traQ |
| QPR74_RS24185 (QPR74_24145) | 62999..63388 | + | 390 | WP_004187479 | DUF6750 family protein | traR |
| QPR74_RS24190 (QPR74_24150) | 63434..63928 | + | 495 | WP_004187480 | hypothetical protein | - |
| QPR74_RS24195 (QPR74_24155) | 63925..66975 | + | 3051 | WP_004187482 | hypothetical protein | traU |
| QPR74_RS24200 (QPR74_24160) | 66972..68180 | + | 1209 | WP_011091082 | conjugal transfer protein TraW | traW |
| QPR74_RS24205 (QPR74_24165) | 68177..68773 | + | 597 | WP_015060003 | hypothetical protein | - |
| QPR74_RS24210 (QPR74_24170) | 68766..70961 | + | 2196 | WP_015062834 | DotA/TraY family protein | traY |
| QPR74_RS24215 (QPR74_24175) | 70963..71616 | + | 654 | WP_015060005 | hypothetical protein | - |
| QPR74_RS24220 (QPR74_24180) | 71697..71927 | + | 231 | WP_011091085 | IncL/M type plasmid replication protein RepC | - |
Host bacterium
| ID | 16253 | GenBank | NZ_CP126769 |
| Plasmid name | pEh23_OXA48 | Incompatibility group | IncL/M |
| Plasmid size | 72222 bp | Coordinate of oriT [Strand] | 48771..48876 [+] |
| Host baterium | Enterobacter hormaechei strain Ehh_23 |
Cargo genes
| Drug resistance gene | blaOXA-48, blaCTX-M-14b, aph(3'')-Ib, aph(3')-VIb, aph(6)-Id |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |