Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 115819 |
| Name | oriT_pEh22_OXA48  |
| Organism | Enterobacter hormaechei strain Ehh_22 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP126776 (42766..42871 [+], 106 nt) |
| oriT length | 106 nt |
| IRs (inverted repeats) | 30..35, 41..46 (CCGCCG..CGGCGG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 106 nt
>oriT_pEh22_OXA48
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 10137 | GenBank | WP_004187323 |
| Name | traI_QPR68_RS25050_pEh22_OXA48 |
UniProt ID | A0A3U8KUL3 |
| Length | 659 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75179.89 Da Isoelectric Point: 9.9948
>WP_004187323.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3U8KUL3 |
T4CP
| ID | 11843 | GenBank | WP_004206885 |
| Name | t4cp2_QPR68_RS24740_pEh22_OXA48 |
UniProt ID | _ |
| Length | 695 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 695 a.a. Molecular weight: 79538.91 Da Isoelectric Point: 4.8317
>WP_004206885.1 MULTISPECIES: conjugal transfer protein TrbC [Enterobacterales]
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 45667..64956
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| QPR68_RS25030 (QPR68_25010) | 40751..41062 | + | 312 | WP_004187333 | hypothetical protein | - |
| QPR68_RS25035 (QPR68_25015) | 41195..41731 | + | 537 | WP_004187332 | hypothetical protein | - |
| QPR68_RS25040 (QPR68_25020) | 42233..42628 | - | 396 | WP_019725163 | hypothetical protein | - |
| QPR68_RS25045 (QPR68_25025) | 42663..43190 | + | 528 | WP_172740549 | plasmid mobilization protein MobA | - |
| QPR68_RS25050 (QPR68_25030) | 43177..45156 | + | 1980 | WP_004187323 | TraI/MobA(P) family conjugative relaxase | - |
| QPR68_RS25055 (QPR68_25035) | 45170..45670 | + | 501 | WP_004187320 | DotD/TraH family lipoprotein | - |
| QPR68_RS25060 (QPR68_25040) | 45667..46446 | + | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| QPR68_RS25065 (QPR68_25045) | 46457..47620 | + | 1164 | WP_004187313 | plasmid transfer ATPase TraJ | virB11 |
| QPR68_RS25070 (QPR68_25050) | 47610..47870 | + | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| QPR68_RS25075 (QPR68_25055) | 47895..51332 | + | 3438 | WP_015586048 | LPD7 domain-containing protein | - |
| QPR68_RS25080 (QPR68_25060) | 51298..51810 | + | 513 | WP_011091071 | hypothetical protein | traL |
| QPR68_RS25085 (QPR68_25065) | 51811..52023 | - | 213 | WP_305953721 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| QPR68_RS25090 (QPR68_25070) | 51995..52405 | - | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| QPR68_RS25095 (QPR68_25075) | 52473..53186 | + | 714 | WP_004187467 | DotI/IcmL family type IV secretion protein | traM |
| QPR68_RS25100 (QPR68_25080) | 53195..54346 | + | 1152 | WP_004187471 | DotH/IcmK family type IV secretion protein | traN |
| QPR68_RS25105 (QPR68_25085) | 54358..55707 | + | 1350 | WP_004187474 | conjugal transfer protein TraO | traO |
| QPR68_RS25110 (QPR68_25090) | 55719..56423 | + | 705 | WP_015060002 | conjugal transfer protein TraP | traP |
| QPR68_RS25115 (QPR68_25095) | 56447..56977 | + | 531 | WP_004187478 | conjugal transfer protein TraQ | traQ |
| QPR68_RS25120 (QPR68_25100) | 56994..57383 | + | 390 | WP_004187479 | DUF6750 family protein | traR |
| QPR68_RS25125 (QPR68_25105) | 57429..57923 | + | 495 | WP_004187480 | hypothetical protein | - |
| QPR68_RS25130 (QPR68_25110) | 57920..60970 | + | 3051 | WP_004187482 | hypothetical protein | traU |
| QPR68_RS25135 (QPR68_25115) | 60967..62175 | + | 1209 | WP_011091082 | conjugal transfer protein TraW | traW |
| QPR68_RS25140 (QPR68_25120) | 62172..62768 | + | 597 | WP_015060003 | hypothetical protein | - |
| QPR68_RS25145 (QPR68_25125) | 62761..64956 | + | 2196 | WP_015062834 | DotA/TraY family protein | traY |
| QPR68_RS25150 (QPR68_25130) | 64958..65611 | + | 654 | WP_015060005 | hypothetical protein | - |
| QPR68_RS25155 (QPR68_25135) | 65692..65922 | + | 231 | WP_011091085 | IncL/M type plasmid replication protein RepC | - |
Host bacterium
| ID | 16252 | GenBank | NZ_CP126776 |
| Plasmid name | pEh22_OXA48 | Incompatibility group | IncL/M |
| Plasmid size | 66217 bp | Coordinate of oriT [Strand] | 42766..42871 [+] |
| Host baterium | Enterobacter hormaechei strain Ehh_22 |
Cargo genes
| Drug resistance gene | blaOXA-48 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |