Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 115816 |
| Name | oriT_pEh20_OXA48  |
| Organism | Enterobacter hormaechei strain Ehh_20 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP126790 (48773..48878 [+], 106 nt) |
| oriT length | 106 nt |
| IRs (inverted repeats) | 30..35, 41..46 (CCGCCG..CGGCGG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 106 nt
>oriT_pEh20_OXA48
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 10135 | GenBank | WP_004187323 |
| Name | traI_QPR81_RS25655_pEh20_OXA48 |
UniProt ID | A0A3U8KUL3 |
| Length | 659 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75179.89 Da Isoelectric Point: 9.9948
>WP_004187323.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3U8KUL3 |
T4CP
| ID | 11841 | GenBank | WP_004206885 |
| Name | t4cp2_QPR81_RS25340_pEh20_OXA48 |
UniProt ID | _ |
| Length | 695 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 695 a.a. Molecular weight: 79538.91 Da Isoelectric Point: 4.8317
>WP_004206885.1 MULTISPECIES: conjugal transfer protein TrbC [Enterobacterales]
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 51674..70963
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| QPR81_RS25635 (QPR81_25630) | 46759..47070 | + | 312 | WP_004187333 | hypothetical protein | - |
| QPR81_RS25640 (QPR81_25635) | 47203..47739 | + | 537 | WP_036970776 | hypothetical protein | - |
| QPR81_RS25645 (QPR81_25640) | 48240..48605 | - | 366 | WP_015586046 | hypothetical protein | - |
| QPR81_RS25650 (QPR81_25645) | 48880..49197 | + | 318 | WP_011091068 | plasmid mobilization protein MobA | - |
| QPR81_RS25655 (QPR81_25650) | 49184..51163 | + | 1980 | WP_004187323 | TraI/MobA(P) family conjugative relaxase | - |
| QPR81_RS25660 (QPR81_25655) | 51177..51677 | + | 501 | WP_004187320 | DotD/TraH family lipoprotein | - |
| QPR81_RS25665 (QPR81_25660) | 51674..52453 | + | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| QPR81_RS25670 (QPR81_25665) | 52464..53627 | + | 1164 | WP_004187313 | plasmid transfer ATPase TraJ | virB11 |
| QPR81_RS25675 (QPR81_25670) | 53617..53877 | + | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| QPR81_RS25680 (QPR81_25675) | 53902..57339 | + | 3438 | WP_015586048 | LPD7 domain-containing protein | - |
| QPR81_RS25685 (QPR81_25680) | 57305..57817 | + | 513 | WP_011091071 | hypothetical protein | traL |
| QPR81_RS25690 (QPR81_25685) | 57818..58030 | - | 213 | WP_305953721 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| QPR81_RS25695 (QPR81_25690) | 58002..58412 | - | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| QPR81_RS25700 (QPR81_25695) | 58480..59193 | + | 714 | WP_004187467 | DotI/IcmL family type IV secretion protein | traM |
| QPR81_RS25705 (QPR81_25700) | 59202..60353 | + | 1152 | WP_004187471 | DotH/IcmK family type IV secretion protein | traN |
| QPR81_RS25710 (QPR81_25705) | 60365..61714 | + | 1350 | WP_004187474 | conjugal transfer protein TraO | traO |
| QPR81_RS25715 (QPR81_25710) | 61726..62430 | + | 705 | WP_015060002 | conjugal transfer protein TraP | traP |
| QPR81_RS25720 (QPR81_25715) | 62454..62984 | + | 531 | WP_004187478 | conjugal transfer protein TraQ | traQ |
| QPR81_RS25725 (QPR81_25720) | 63001..63390 | + | 390 | WP_004187479 | DUF6750 family protein | traR |
| QPR81_RS25730 (QPR81_25725) | 63436..63930 | + | 495 | WP_004187480 | hypothetical protein | - |
| QPR81_RS25735 (QPR81_25730) | 63927..66977 | + | 3051 | WP_004187482 | hypothetical protein | traU |
| QPR81_RS25740 (QPR81_25735) | 66974..68182 | + | 1209 | WP_011091082 | conjugal transfer protein TraW | traW |
| QPR81_RS25745 (QPR81_25740) | 68179..68775 | + | 597 | WP_015060003 | hypothetical protein | - |
| QPR81_RS25750 (QPR81_25745) | 68768..70963 | + | 2196 | WP_015062834 | DotA/TraY family protein | traY |
| QPR81_RS25755 (QPR81_25750) | 70965..71618 | + | 654 | WP_015060005 | hypothetical protein | - |
| QPR81_RS25760 (QPR81_25755) | 71699..71929 | + | 231 | WP_011091085 | IncL/M type plasmid replication protein RepC | - |
Host bacterium
| ID | 16249 | GenBank | NZ_CP126790 |
| Plasmid name | pEh20_OXA48 | Incompatibility group | IncL/M |
| Plasmid size | 72224 bp | Coordinate of oriT [Strand] | 48773..48878 [+] |
| Host baterium | Enterobacter hormaechei strain Ehh_20 |
Cargo genes
| Drug resistance gene | blaOXA-48, blaCTX-M-14b, aph(3'')-Ib, aph(3')-VIb, aph(6)-Id |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |