Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   115806
Name   oriT_contig000002 in_silico
Organism   Enterococcus faecalis isolate WE0254
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_OU538996 (34141..34190 [+], 50 nt)
oriT length   50 nt
IRs (inverted repeats)     _
Location of nic site      34..35
Conserved sequence flanking the
  nic site    		  GCTTGCAAAA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 50 nt

>oriT_contig000002
AATATCGCAACATGCTAGCATGTTGCTCCGCTTGCAAAAAGAAAGCCTAC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   10126 GenBank   WP_010784826
Name   Relaxase_KG893_RS14135_contig000002 insolico UniProt ID   A0A8E7CAC2
Length   561 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 561 a.a.        Molecular weight: 65352.94 Da        Isoelectric Point: 6.0135

>WP_010784826.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Enterococcus]
MVYTKHFVIHTFDKLNNACSYIENAEKTEVTNDNPSEHLEHLFQYIVNDDKTYMKKLVSGHGIVDPTNPY
EEFKLTKLQAAIQRKIGYTFDPKSERLLPPTLTELEKGNAVLAHHLIQSFSPEDDLTPEKIHEIGYNTVM
ELTGGEYEFVIATHVDKEHLHNHIIFSSTNLKTGKAFRWQKGTKKVFEQISDKIAAKEGAKIIEKSPKQT
HTKYTKWQTESIYKQKIKSRLDFLLEQSSSINDFLEKAEALNLSADFSNKWTTYRLLDEPQIKNTRSRSL
SKSDPTRYNYEKIIERLKENKNVLTVEECVKRYEEKNEQEKNNFDYQFTIEPWQLSHKTERGYYINIDYG
YGNSGKLFVGGYKVDPLDNGNYNIYIKRNEYFYFMSDKDATKSKYLTGASLIKQLRLYNGQTPLKREPVM
QTIDELVSAINFLAANEIEDTRQLKLLEEKLEVAFLEAEKTLETLDEKMLELHQLSNLLLENELQGDPEL
IQKKLKTLLPDATLAEFSYEDVRGEIEAIKTSQTLLESKLERTRNEINQLHEIQAVQEKEPEKQTDIKPK
L

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(37-296)


  Protein structure



No available structure.




Auxiliary protein

ID   5961 GenBank   WP_002393737
Name   WP_002393737_contig000002 insolico UniProt ID   A0A8E7CBI6
Length   118 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 118 a.a.        Molecular weight: 13965.01 Da        Isoelectric Point: 9.6545

>WP_002393737.1 MULTISPECIES: plasmid mobilization relaxosome protein MobC [Enterococcus]
MENKQKLKRPIQRIVRLSEEENNLIKRKIEESFFPNFQNFALHLLIQGEIRHVDYSELNRLTTEIHKIGI
NINQMARLANQFHEISSEDIKDLTDKVQSLNALVQSELNKLIKRKDQS

  Protein domains


Predicted by InterproScan.

Bacterial mobilisation protein (MobC) (MobC)

(64-105)


  Protein structure



No available structure.




T4CP

ID   11828 GenBank   WP_126263104
Name   t4cp2_KG893_RS14115_contig000002 insolico UniProt ID   _
Length   609 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 609 a.a.        Molecular weight: 70188.16 Da        Isoelectric Point: 6.1807

>WP_126263104.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Enterococcus]
MQTYKKELKPYLYLGIFLFVIGFGIGNFFWLLPGTDYFSKTNYLFTKDILTYIEQTFYRFFITPSLLGFS
VGILGFLLGLLMYVRDNDRGIYRHGEEYGSARFATPAEMKKYEDPIPENNIIVSKHVKISLFNKRLPIKL
QKNKNIAILGDSGAAKTLAFIKTNLMQRHASFITTDPDGGILPEIGLLLKKGKYKIKVLDLNTLSNSDTF
NVFEYMHSELDVDRVLEAVTEATKEVEKTTGDDFWIKAEGLLVRSLIAYLWFDGRRNDYTPHLGMIADML
RHLKRKDKNVPSPVEEWFEELNEAIPDNYAYRQWTLFNNLYESETRASVLGIAAARYSVFDHEEVVNMIR
TDTMDIDSWNEEKTAVFIAIPETNTSFNFIAALMFATVGEVLRNKSDQVRLGKRVLEEGKELLHVRFLID
EFANIGRIPHFEKMLTTFRKREMSFTIVLQSLNQLQTLYRNGWQNILNGCACLLYLGGDEEETTKYLSAR
AGKQTLSIRKHSMNKGNQGGGSENRDKYGRDLIDRSEVALIGGDECLVFISKEHVFKDKKFYAFQHDRAD
ELATGPTDDKWYTWRRYMTDEEAILDKISATDIIDHGEITSEQKEETHV

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(144-558)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 12755..31497

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KG893_RS14000 (WE0254_02873) 7870..8250 + 381 WP_002367664 hypothetical protein -
KG893_RS14005 (WE0254_02874) 8821..9126 + 306 WP_126263119 hypothetical protein -
KG893_RS14010 (WE0254_02875) 9137..11809 + 2673 WP_010706919 surface exclusion protein SEA1/PrgA -
KG893_RS14015 (WE0254_02876) 11888..12082 + 195 WP_002415094 hypothetical protein -
KG893_RS14020 12355..12543 + 189 Protein_10 hypothetical protein -
KG893_RS14025 (WE0254_02877) 12755..16672 + 3918 WP_221595589 LPXTG-anchored aggregation substance PrgB/Asc10 prgB
KG893_RS14030 (WE0254_02878) 16734..17090 + 357 WP_002367672 pheromone response system RNA-binding regulator PrgU -
KG893_RS14035 (WE0254_02879) 17118..17975 + 858 WP_002367673 pheromone response LPXTG-anchored adhesin PrgC prgC
KG893_RS14040 (WE0254_02880) 18017..18916 + 900 WP_002409287 hypothetical protein -
KG893_RS14045 (WE0254_02881) 18936..19370 + 435 WP_002365857 hypothetical protein -
KG893_RS14050 (WE0254_02882) 19417..19644 + 228 WP_002365858 hypothetical protein -
KG893_RS14055 (WE0254_02883) 19658..19954 + 297 WP_002387740 hypothetical protein -
KG893_RS14060 (WE0254_02884) 19969..20769 + 801 WP_002393746 hypothetical protein prgHb
KG893_RS14065 (WE0254_02885) 20771..21124 + 354 WP_002367919 PrgI family mobile element protein prgIc
KG893_RS14070 (WE0254_02886) 21078..23468 + 2391 WP_002365863 VirB4-like conjugal transfer ATPase, CD1110 family virb4
KG893_RS14075 (WE0254_02887) 23480..26095 + 2616 WP_002410288 phage tail tip lysozyme prgK
KG893_RS14080 (WE0254_02888) 26119..26745 + 627 WP_002386490 hypothetical protein prgL
KG893_RS14085 (WE0254_02889) 26732..26977 + 246 WP_002365866 hypothetical protein -
KG893_RS14090 (WE0254_02890) 26961..27569 + 609 WP_016618746 hypothetical protein -
KG893_RS14095 (WE0254_02891) 27683..28438 + 756 WP_077932946 Fic family protein -
KG893_RS14100 (WE0254_02892) 28455..28913 + 459 WP_077932945 single-stranded DNA-binding protein -
KG893_RS14105 (WE0254_02893) 28959..29141 + 183 WP_002367689 hypothetical protein -
KG893_RS14110 (WE0254_02894) 29183..29668 + 486 WP_002386493 PcfB family protein gbs1365
KG893_RS14115 (WE0254_02895) 29668..31497 + 1830 WP_126263104 VirD4-like conjugal transfer protein, CD1115 family virb4
KG893_RS14120 (WE0254_02896) 31547..33706 + 2160 WP_126263105 ArdC-like ssDNA-binding domain-containing protein -
KG893_RS14125 (WE0254_02897) 33739..34011 + 273 WP_002362409 LtrD -
KG893_RS14130 (WE0254_02898) 34257..34613 + 357 WP_002393737 plasmid mobilization relaxosome protein MobC -
KG893_RS14135 (WE0254_02899) 34614..36299 + 1686 WP_010784826 relaxase/mobilization nuclease domain-containing protein -

Host bacterium

ID   16239 GenBank   NZ_OU538996
Plasmid name   contig000002 Incompatibility group   -
Plasmid size   80496 bp Coordinate of oriT [Strand]   34141..34190 [+]
Host baterium   Enterococcus faecalis isolate WE0254

Cargo genes

Drug resistance gene   ant(9)-Ia, erm(A), fexA, optrA
Virulence gene   prgB/asc10
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIA9