Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 115805
• Name: oriT_pHDC14-2.35K
• Organism: Enterococcus hirae strain HDC14-2
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP042293 (5892..5991 [-], 100 nt)
• oriT length: 100 nt
• IRs (inverted repeats): 13..18, 32..37 (AAAGGG..CCCTTT)
• Location of nic site: 94..95
• Conserved sequence flanking the nic site: GCTTGCAGTA
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 100 nt

>oriT_pHDC14-2.35K
CCGAAGGGGATGAAAGGGAACGTGTAACTTGCCCTTTTCCAAGCAACTTTGTCATACAAGACACTTCGTTATTGTATGACAAAGTGAAAGCTTGCAGTAT

oriT secondary structure

Predicted by RNAfold.

Relaxase

Relaxase_FQ488_RS15045_pHDC14-2.35K

• ID: 10125
• GenBank: WP_115250366
• Name: Relaxase_FQ488_RS15045_pHDC14-2.35K
• UniProt ID: _
• Length: 345 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 345 a.a.
• Molecular weight: 40658.12 Da
• Isoelectric Point: 10.2506

>WP_115250366.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Enterococcus]
MREIHNKTQERNQVLRITQSFALDELSPKNTQDWQRANDLGVALAEKLYPQHQTAVYTHLDGQNHVLHNH
ILVNKVNLETGKKLREVPGKTVEKARVINDRLAQQQHWRILPPPQERFSETEQDLVAKQKYSYMDDLRTR
INEAMVDISVRSYKDLRNILEQKSIIFQERVQSLSYAFLDVNNKKRRAKGARLGTDFEKETIFNELENRA
RQQQPRPIEPVKQRELATTRLMQDTQQTEPSLERRESTTRGIEQQLEQRKPGIARLIDTIQHLRDQLPGI
TQLVKKHLTYAKEALFDAFEKRFSFDMAQHKQVQRKKIQQDLEQRTQEQAMPKFQTPTQDRGLSL

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (2-208)

Protein structure

No available structure.

Host bacterium

• ID: 16238
• GenBank: NZ_CP042293
• Plasmid name: pHDC14-2.35K
• Incompatibility group: -
• Plasmid size: 35046 bp
• Coordinate of oriT [Strand]: 5892..5991 [-]
• Host baterium: Enterococcus hirae strain HDC14-2

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: AcrIIA1, AcrIIA21