Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 115791 |
| Name | oriT_99202523580|unnamed2  |
| Organism | Enterobacter ludwigii strain 99202523580 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP134673 (55282..55368 [-], 87 nt) |
| oriT length | 87 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 87 nt
>oriT_99202523580|unnamed2
GGGTGTCGGGGCGGAGCCCTGACCAGGTGGCATTTGTAATATCGTGCGTGCGCGGTATTACAAATGCACATCCTGTCCCGTCTTTCG
GGGTGTCGGGGCGGAGCCCTGACCAGGTGGCATTTGTAATATCGTGCGTGCGCGGTATTACAAATGCACATCCTGTCCCGTCTTTCG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 10115 | GenBank | WP_311382345 |
| Name | Relaxase_RIK62_RS23615_99202523580|unnamed2 |
UniProt ID | _ |
| Length | 899 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 899 a.a. Molecular weight: 102354.55 Da Isoelectric Point: 8.3892
>WP_311382345.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Enterobacter cloacae complex]
MIPVIPPKRQDGKSSFGDLVSYVSVRDEPRDDDLMEAVKASGTKPEMPHSSRFSRLVDYATKLRDESFIS
LVDVMPDGGEWVNFYGVTCFHNCTSIESAAEEMEFSARKAKFAHNKSDPVFHYILSWQSHESPRPEQIYD
SVSHTLSRLGLVGHQFVAAVHTDTDNLHVHIAVNRVHPETGYLNRLAYSQEKLSKACRELELKHGFSPDN
GCYVIAPDNRIVRRTSVERDRQSAWKRDRNQSLREYIADTAIAGLRESPVTDWSSLHQRFAKEGLFLTKD
SGELKVKDGWNSERPGVTLSAFGHAFDTDKLTRKLGEFVPPAQDIFSQVPAVGRYEPDKIIMPSRPERMT
EKESLSDYATARLRAPLIALDQDPTQRTIQSVHTLLAQSGLYLKEQHDRLVICDGYDPTRTPVRAERVWP
ALTKAILDSYKGGWQPVPKDIFQQVPPAEQFTGGGLEATPVSDREWRKLRTGSGPQGALKREIFSDKESL
WGYAVSHCRQDIEMLIGSKQFSWERCHELLARQGLLLMREQQGLVVMDAYNHEQTPVKASHVHPDLTLAR
AEPHAGSFVSVPADIFERVKPVSRYNPELAVSDRDIPGMKRDPELRRQRREARAAAREDLKARYAAWRAN
WQRPDLHGRERYQAIHDECRMRKARIRVEHRDPLVRKLHYHIAELQRMQALIALKETMKAERAELIGQGK
WYPPSYRQWVEAEAVKGDKAAISQLRGWDYRDRRGNAVKSTTDNRCVVICEPGGTPMYSGVPGLRASLKK
NGRVQFRDPETGRHICTDYGDRVIFRNSGDYDALKRDMVKVAPVLFSRSPSVAMAPEGNNEQFNTAFAKM
VAWHNVNHRDEGEYQISRPEVDQVRVESEMLFAEMTNQGSNTEAVRNEPGPNWKPPSPL
MIPVIPPKRQDGKSSFGDLVSYVSVRDEPRDDDLMEAVKASGTKPEMPHSSRFSRLVDYATKLRDESFIS
LVDVMPDGGEWVNFYGVTCFHNCTSIESAAEEMEFSARKAKFAHNKSDPVFHYILSWQSHESPRPEQIYD
SVSHTLSRLGLVGHQFVAAVHTDTDNLHVHIAVNRVHPETGYLNRLAYSQEKLSKACRELELKHGFSPDN
GCYVIAPDNRIVRRTSVERDRQSAWKRDRNQSLREYIADTAIAGLRESPVTDWSSLHQRFAKEGLFLTKD
SGELKVKDGWNSERPGVTLSAFGHAFDTDKLTRKLGEFVPPAQDIFSQVPAVGRYEPDKIIMPSRPERMT
EKESLSDYATARLRAPLIALDQDPTQRTIQSVHTLLAQSGLYLKEQHDRLVICDGYDPTRTPVRAERVWP
ALTKAILDSYKGGWQPVPKDIFQQVPPAEQFTGGGLEATPVSDREWRKLRTGSGPQGALKREIFSDKESL
WGYAVSHCRQDIEMLIGSKQFSWERCHELLARQGLLLMREQQGLVVMDAYNHEQTPVKASHVHPDLTLAR
AEPHAGSFVSVPADIFERVKPVSRYNPELAVSDRDIPGMKRDPELRRQRREARAAAREDLKARYAAWRAN
WQRPDLHGRERYQAIHDECRMRKARIRVEHRDPLVRKLHYHIAELQRMQALIALKETMKAERAELIGQGK
WYPPSYRQWVEAEAVKGDKAAISQLRGWDYRDRRGNAVKSTTDNRCVVICEPGGTPMYSGVPGLRASLKK
NGRVQFRDPETGRHICTDYGDRVIFRNSGDYDALKRDMVKVAPVLFSRSPSVAMAPEGNNEQFNTAFAKM
VAWHNVNHRDEGEYQISRPEVDQVRVESEMLFAEMTNQGSNTEAVRNEPGPNWKPPSPL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 11820 | GenBank | WP_311382348 |
| Name | trbC_RIK62_RS23635_99202523580|unnamed2 |
UniProt ID | _ |
| Length | 766 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 766 a.a. Molecular weight: 87431.70 Da Isoelectric Point: 6.4590
>WP_311382348.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacter cloacae complex]
MNNTPVNQALMKRNAWNSPVLELMRDHLLYAGIMAGSVVAGFIWPLAIPLLLLLAIVASISFGTHRWRMP
MRMPVHLNKVDPSEDRKVRRSLFRVFPSLFQYETTQESIGKGIFYMGYKRMNDIGRELWLTLDDLTRHVM
FFASTGGGKTETIYAWMLNSFCWGRGFTFVDGKAQNDTARTCYYLARRFGREDDVEYINFMNSGMSRSEM
IQNGDKSRPQSHQWNPFCYSTEAFVAETMQSMLPTNVQGGEWQSRAIAMNKALVFGTKFYCVRENKTMSL
QLLREFMPLEKLAGLYCRAVDDQWPEEAVSPLYNYLVDVPGFDMATVRQPSAWTEEPRKQHSYLTGQFLE
TFNTFTETFGNIFAEDAGDIDIRDSIHSDRILLVLIPAMNTSQHTTSALGRMFVTQQSMILARDLGHKLE
GLDSEALEITKYKGQFPYMNYLDEVGAYYTERIAVQATQVRSLEFALIMMSQDQERIENQTSAANVATLM
QNAGTKVAGKIVSDDKTARTISNAAGQEARANMVSLQRQDGIIGTSWIDGDHINIQMENKINVQDLIKLQ
PGENYTVFQGDPVPGASFYIESQDKTCNAPVIINRYITINPPNLKQLRKLVPRTAQRRLPAPENVSKIIG
VLTEKPSRRRKAARAEPWKIIDTFQQRLANRQEAHNLMTEYDMDHSGREENLWEEALEIIRTTTMEERTI
RYITLNKPESESTEVNAEEIKLAPDAILRTLTLPQPAYVPPARYRNEPFISPKLPGSPHPDMEWPE
MNNTPVNQALMKRNAWNSPVLELMRDHLLYAGIMAGSVVAGFIWPLAIPLLLLLAIVASISFGTHRWRMP
MRMPVHLNKVDPSEDRKVRRSLFRVFPSLFQYETTQESIGKGIFYMGYKRMNDIGRELWLTLDDLTRHVM
FFASTGGGKTETIYAWMLNSFCWGRGFTFVDGKAQNDTARTCYYLARRFGREDDVEYINFMNSGMSRSEM
IQNGDKSRPQSHQWNPFCYSTEAFVAETMQSMLPTNVQGGEWQSRAIAMNKALVFGTKFYCVRENKTMSL
QLLREFMPLEKLAGLYCRAVDDQWPEEAVSPLYNYLVDVPGFDMATVRQPSAWTEEPRKQHSYLTGQFLE
TFNTFTETFGNIFAEDAGDIDIRDSIHSDRILLVLIPAMNTSQHTTSALGRMFVTQQSMILARDLGHKLE
GLDSEALEITKYKGQFPYMNYLDEVGAYYTERIAVQATQVRSLEFALIMMSQDQERIENQTSAANVATLM
QNAGTKVAGKIVSDDKTARTISNAAGQEARANMVSLQRQDGIIGTSWIDGDHINIQMENKINVQDLIKLQ
PGENYTVFQGDPVPGASFYIESQDKTCNAPVIINRYITINPPNLKQLRKLVPRTAQRRLPAPENVSKIIG
VLTEKPSRRRKAARAEPWKIIDTFQQRLANRQEAHNLMTEYDMDHSGREENLWEEALEIIRTTTMEERTI
RYITLNKPESESTEVNAEEIKLAPDAILRTLTLPQPAYVPPARYRNEPFISPKLPGSPHPDMEWPE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 61920..84960
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| RIK62_RS23620 (RIK62_23620) | 58507..58737 | - | 231 | WP_311382346 | hypothetical protein | - |
| RIK62_RS23625 (RIK62_23625) | 58846..59130 | - | 285 | WP_311382347 | hypothetical protein | - |
| RIK62_RS23630 (RIK62_23630) | 59274..59543 | - | 270 | WP_048210058 | hypothetical protein | - |
| RIK62_RS23635 (RIK62_23635) | 59616..61916 | - | 2301 | WP_311382348 | F-type conjugative transfer protein TrbC | - |
| RIK62_RS23640 (RIK62_23640) | 61920..63116 | - | 1197 | WP_311382349 | thioredoxin fold domain-containing protein | trbB |
| RIK62_RS23645 (RIK62_23645) | 63169..64377 | - | 1209 | WP_311382350 | conjugal transfer protein TrbA | trbA |
| RIK62_RS23650 (RIK62_23650) | 64690..65322 | - | 633 | WP_311382351 | plasmid IncI1-type surface exclusion protein ExcA | - |
| RIK62_RS23655 (RIK62_23655) | 65383..67545 | - | 2163 | WP_311382352 | DotA/TraY family protein | traY |
| RIK62_RS23660 (RIK62_23660) | 67603..68145 | - | 543 | WP_053070179 | conjugal transfer protein TraX | - |
| RIK62_RS23665 (RIK62_23665) | 68142..69344 | - | 1203 | WP_048210062 | conjugal transfer protein TraW | traW |
| RIK62_RS23670 (RIK62_23670) | 69357..69926 | - | 570 | WP_284951006 | conjugal transfer protein TraV | traV |
| RIK62_RS23675 (RIK62_23675) | 69926..72979 | - | 3054 | WP_311382353 | ATP-binding protein | traU |
| RIK62_RS23680 (RIK62_23680) | 73060..73929 | - | 870 | WP_311382354 | conjugal transfer protein TraT | traT |
| RIK62_RS23685 (RIK62_23685) | 73976..74380 | - | 405 | WP_172689581 | DUF6750 family protein | traR |
| RIK62_RS23690 (RIK62_23690) | 74415..74939 | - | 525 | WP_172689580 | conjugal transfer protein TraQ | traQ |
| RIK62_RS23695 (RIK62_23695) | 74939..75709 | - | 771 | WP_311382355 | conjugal transfer protein TraP | traP |
| RIK62_RS23700 (RIK62_23700) | 75693..77009 | - | 1317 | WP_311382356 | conjugal transfer protein TraO | traO |
| RIK62_RS23705 (RIK62_23705) | 77009..77992 | - | 984 | WP_284951011 | DotH/IcmK family type IV secretion protein | traN |
| RIK62_RS23710 (RIK62_23710) | 78003..78704 | - | 702 | WP_284951012 | DotI/IcmL/TraM family protein | traM |
| RIK62_RS23715 (RIK62_23715) | 78710..79060 | - | 351 | WP_048210070 | hypothetical protein | traL |
| RIK62_RS23720 (RIK62_23720) | 79078..82626 | - | 3549 | WP_311382357 | LPD7 domain-containing protein | - |
| RIK62_RS23725 (RIK62_23725) | 82698..82985 | - | 288 | WP_048210072 | IcmT/TraK family protein | traK |
| RIK62_RS23730 (RIK62_23730) | 82982..84142 | - | 1161 | WP_311382358 | plasmid transfer ATPase TraJ | virB11 |
| RIK62_RS23735 (RIK62_23735) | 84154..84960 | - | 807 | WP_172689573 | type IV secretory system conjugative DNA transfer family protein | traI |
| RIK62_RS23740 (RIK62_23740) | 84957..85430 | - | 474 | WP_311382359 | DotD/TraH family lipoprotein | - |
| RIK62_RS23745 (RIK62_23745) | 85518..86036 | - | 519 | WP_172689571 | hypothetical protein | - |
Host bacterium
| ID | 16224 | GenBank | NZ_CP134673 |
| Plasmid name | 99202523580|unnamed2 | Incompatibility group | - |
| Plasmid size | 86232 bp | Coordinate of oriT [Strand] | 55282..55368 [-] |
| Host baterium | Enterobacter ludwigii strain 99202523580 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |