Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 115790 |
| Name | oriT_99202522619|unnamed2  |
| Organism | Enterobacter ludwigii strain 99202522619 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP134669 (69251..69337 [-], 87 nt) |
| oriT length | 87 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 87 nt
>oriT_99202522619|unnamed2
GGGTGTCGGGGCGGAGCCCTGACCAGGTGGCATTTGTAATATCGTGCGTGCGCGGTATTACAAATGCACATCCTGTCCCGTCTTTCG
GGGTGTCGGGGCGGAGCCCTGACCAGGTGGCATTTGTAATATCGTGCGTGCGCGGTATTACAAATGCACATCCTGTCCCGTCTTTCG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 10114 | GenBank | WP_311382345 |
| Name | Relaxase_RIK67_RS01050_99202522619|unnamed2 |
UniProt ID | _ |
| Length | 899 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 899 a.a. Molecular weight: 102354.55 Da Isoelectric Point: 8.3892
>WP_311382345.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Enterobacter cloacae complex]
MIPVIPPKRQDGKSSFGDLVSYVSVRDEPRDDDLMEAVKASGTKPEMPHSSRFSRLVDYATKLRDESFIS
LVDVMPDGGEWVNFYGVTCFHNCTSIESAAEEMEFSARKAKFAHNKSDPVFHYILSWQSHESPRPEQIYD
SVSHTLSRLGLVGHQFVAAVHTDTDNLHVHIAVNRVHPETGYLNRLAYSQEKLSKACRELELKHGFSPDN
GCYVIAPDNRIVRRTSVERDRQSAWKRDRNQSLREYIADTAIAGLRESPVTDWSSLHQRFAKEGLFLTKD
SGELKVKDGWNSERPGVTLSAFGHAFDTDKLTRKLGEFVPPAQDIFSQVPAVGRYEPDKIIMPSRPERMT
EKESLSDYATARLRAPLIALDQDPTQRTIQSVHTLLAQSGLYLKEQHDRLVICDGYDPTRTPVRAERVWP
ALTKAILDSYKGGWQPVPKDIFQQVPPAEQFTGGGLEATPVSDREWRKLRTGSGPQGALKREIFSDKESL
WGYAVSHCRQDIEMLIGSKQFSWERCHELLARQGLLLMREQQGLVVMDAYNHEQTPVKASHVHPDLTLAR
AEPHAGSFVSVPADIFERVKPVSRYNPELAVSDRDIPGMKRDPELRRQRREARAAAREDLKARYAAWRAN
WQRPDLHGRERYQAIHDECRMRKARIRVEHRDPLVRKLHYHIAELQRMQALIALKETMKAERAELIGQGK
WYPPSYRQWVEAEAVKGDKAAISQLRGWDYRDRRGNAVKSTTDNRCVVICEPGGTPMYSGVPGLRASLKK
NGRVQFRDPETGRHICTDYGDRVIFRNSGDYDALKRDMVKVAPVLFSRSPSVAMAPEGNNEQFNTAFAKM
VAWHNVNHRDEGEYQISRPEVDQVRVESEMLFAEMTNQGSNTEAVRNEPGPNWKPPSPL
MIPVIPPKRQDGKSSFGDLVSYVSVRDEPRDDDLMEAVKASGTKPEMPHSSRFSRLVDYATKLRDESFIS
LVDVMPDGGEWVNFYGVTCFHNCTSIESAAEEMEFSARKAKFAHNKSDPVFHYILSWQSHESPRPEQIYD
SVSHTLSRLGLVGHQFVAAVHTDTDNLHVHIAVNRVHPETGYLNRLAYSQEKLSKACRELELKHGFSPDN
GCYVIAPDNRIVRRTSVERDRQSAWKRDRNQSLREYIADTAIAGLRESPVTDWSSLHQRFAKEGLFLTKD
SGELKVKDGWNSERPGVTLSAFGHAFDTDKLTRKLGEFVPPAQDIFSQVPAVGRYEPDKIIMPSRPERMT
EKESLSDYATARLRAPLIALDQDPTQRTIQSVHTLLAQSGLYLKEQHDRLVICDGYDPTRTPVRAERVWP
ALTKAILDSYKGGWQPVPKDIFQQVPPAEQFTGGGLEATPVSDREWRKLRTGSGPQGALKREIFSDKESL
WGYAVSHCRQDIEMLIGSKQFSWERCHELLARQGLLLMREQQGLVVMDAYNHEQTPVKASHVHPDLTLAR
AEPHAGSFVSVPADIFERVKPVSRYNPELAVSDRDIPGMKRDPELRRQRREARAAAREDLKARYAAWRAN
WQRPDLHGRERYQAIHDECRMRKARIRVEHRDPLVRKLHYHIAELQRMQALIALKETMKAERAELIGQGK
WYPPSYRQWVEAEAVKGDKAAISQLRGWDYRDRRGNAVKSTTDNRCVVICEPGGTPMYSGVPGLRASLKK
NGRVQFRDPETGRHICTDYGDRVIFRNSGDYDALKRDMVKVAPVLFSRSPSVAMAPEGNNEQFNTAFAKM
VAWHNVNHRDEGEYQISRPEVDQVRVESEMLFAEMTNQGSNTEAVRNEPGPNWKPPSPL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 11819 | GenBank | WP_311382348 |
| Name | trbC_RIK67_RS01070_99202522619|unnamed2 |
UniProt ID | _ |
| Length | 766 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 766 a.a. Molecular weight: 87431.70 Da Isoelectric Point: 6.4590
>WP_311382348.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacter cloacae complex]
MNNTPVNQALMKRNAWNSPVLELMRDHLLYAGIMAGSVVAGFIWPLAIPLLLLLAIVASISFGTHRWRMP
MRMPVHLNKVDPSEDRKVRRSLFRVFPSLFQYETTQESIGKGIFYMGYKRMNDIGRELWLTLDDLTRHVM
FFASTGGGKTETIYAWMLNSFCWGRGFTFVDGKAQNDTARTCYYLARRFGREDDVEYINFMNSGMSRSEM
IQNGDKSRPQSHQWNPFCYSTEAFVAETMQSMLPTNVQGGEWQSRAIAMNKALVFGTKFYCVRENKTMSL
QLLREFMPLEKLAGLYCRAVDDQWPEEAVSPLYNYLVDVPGFDMATVRQPSAWTEEPRKQHSYLTGQFLE
TFNTFTETFGNIFAEDAGDIDIRDSIHSDRILLVLIPAMNTSQHTTSALGRMFVTQQSMILARDLGHKLE
GLDSEALEITKYKGQFPYMNYLDEVGAYYTERIAVQATQVRSLEFALIMMSQDQERIENQTSAANVATLM
QNAGTKVAGKIVSDDKTARTISNAAGQEARANMVSLQRQDGIIGTSWIDGDHINIQMENKINVQDLIKLQ
PGENYTVFQGDPVPGASFYIESQDKTCNAPVIINRYITINPPNLKQLRKLVPRTAQRRLPAPENVSKIIG
VLTEKPSRRRKAARAEPWKIIDTFQQRLANRQEAHNLMTEYDMDHSGREENLWEEALEIIRTTTMEERTI
RYITLNKPESESTEVNAEEIKLAPDAILRTLTLPQPAYVPPARYRNEPFISPKLPGSPHPDMEWPE
MNNTPVNQALMKRNAWNSPVLELMRDHLLYAGIMAGSVVAGFIWPLAIPLLLLLAIVASISFGTHRWRMP
MRMPVHLNKVDPSEDRKVRRSLFRVFPSLFQYETTQESIGKGIFYMGYKRMNDIGRELWLTLDDLTRHVM
FFASTGGGKTETIYAWMLNSFCWGRGFTFVDGKAQNDTARTCYYLARRFGREDDVEYINFMNSGMSRSEM
IQNGDKSRPQSHQWNPFCYSTEAFVAETMQSMLPTNVQGGEWQSRAIAMNKALVFGTKFYCVRENKTMSL
QLLREFMPLEKLAGLYCRAVDDQWPEEAVSPLYNYLVDVPGFDMATVRQPSAWTEEPRKQHSYLTGQFLE
TFNTFTETFGNIFAEDAGDIDIRDSIHSDRILLVLIPAMNTSQHTTSALGRMFVTQQSMILARDLGHKLE
GLDSEALEITKYKGQFPYMNYLDEVGAYYTERIAVQATQVRSLEFALIMMSQDQERIENQTSAANVATLM
QNAGTKVAGKIVSDDKTARTISNAAGQEARANMVSLQRQDGIIGTSWIDGDHINIQMENKINVQDLIKLQ
PGENYTVFQGDPVPGASFYIESQDKTCNAPVIINRYITINPPNLKQLRKLVPRTAQRRLPAPENVSKIIG
VLTEKPSRRRKAARAEPWKIIDTFQQRLANRQEAHNLMTEYDMDHSGREENLWEEALEIIRTTTMEERTI
RYITLNKPESESTEVNAEEIKLAPDAILRTLTLPQPAYVPPARYRNEPFISPKLPGSPHPDMEWPE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 512..24155
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| RIK67_RS00655 (RIK67_00655) | 512..1381 | - | 870 | WP_311382354 | conjugal transfer protein TraT | traT |
| RIK67_RS00660 (RIK67_00660) | 1428..1832 | - | 405 | WP_172689581 | DUF6750 family protein | traR |
| RIK67_RS00665 (RIK67_00665) | 1867..2391 | - | 525 | WP_172689580 | conjugal transfer protein TraQ | traQ |
| RIK67_RS00670 (RIK67_00670) | 2391..3161 | - | 771 | WP_311382355 | conjugal transfer protein TraP | traP |
| RIK67_RS00675 (RIK67_00675) | 3145..4461 | - | 1317 | WP_311382356 | conjugal transfer protein TraO | traO |
| RIK67_RS00680 (RIK67_00680) | 4461..5444 | - | 984 | WP_284951011 | DotH/IcmK family type IV secretion protein | traN |
| RIK67_RS00685 (RIK67_00685) | 5455..6156 | - | 702 | WP_284951012 | DotI/IcmL/TraM family protein | traM |
| RIK67_RS00690 (RIK67_00690) | 6162..6512 | - | 351 | WP_048210070 | hypothetical protein | traL |
| RIK67_RS00695 (RIK67_00695) | 6530..10078 | - | 3549 | WP_311382357 | LPD7 domain-containing protein | - |
| RIK67_RS00700 (RIK67_00700) | 10150..10437 | - | 288 | WP_048210072 | IcmT/TraK family protein | traK |
| RIK67_RS00705 (RIK67_00705) | 10434..11594 | - | 1161 | WP_311382358 | plasmid transfer ATPase TraJ | virB11 |
| RIK67_RS00710 (RIK67_00710) | 11606..12412 | - | 807 | WP_172689573 | type IV secretory system conjugative DNA transfer family protein | traI |
| RIK67_RS00715 (RIK67_00715) | 12409..12882 | - | 474 | WP_311382359 | DotD/TraH family lipoprotein | - |
| RIK67_RS00720 (RIK67_00720) | 12970..13488 | - | 519 | WP_172689571 | hypothetical protein | - |
| RIK67_RS00725 (RIK67_00725) | 13509..14717 | - | 1209 | WP_172689570 | conjugal transfer protein TraF | - |
| RIK67_RS00730 (RIK67_00730) | 14820..15656 | - | 837 | WP_172689569 | hypothetical protein | traE |
| RIK67_RS00735 (RIK67_00735) | 15802..16278 | - | 477 | WP_156174862 | hypothetical protein | - |
| RIK67_RS00740 (RIK67_00740) | 16298..17458 | - | 1161 | WP_048210079 | site-specific integrase | - |
| RIK67_RS00745 (RIK67_00745) | 18321..19658 | - | 1338 | WP_311382360 | shufflon system plasmid conjugative transfer pilus tip adhesin PilV | - |
| RIK67_RS00750 (RIK67_00750) | 19655..20329 | - | 675 | WP_284950938 | prepilin peptidase | - |
| RIK67_RS00755 (RIK67_00755) | 20314..20796 | - | 483 | WP_284951020 | lytic transglycosylase domain-containing protein | virB1 |
| RIK67_RS00760 (RIK67_00760) | 20881..21495 | - | 615 | WP_311382361 | type 4 pilus major pilin | - |
| RIK67_RS00765 (RIK67_00765) | 21509..22603 | - | 1095 | WP_311382374 | type II secretion system F family protein | - |
| RIK67_RS00770 (RIK67_00770) | 22596..24155 | - | 1560 | WP_311382362 | ATPase, T2SS/T4P/T4SS family | virB11 |
| RIK67_RS00775 (RIK67_00775) | 24158..24673 | - | 516 | WP_311382363 | type IV pilus biogenesis protein PilP | - |
| RIK67_RS00780 (RIK67_00780) | 24657..25961 | - | 1305 | WP_311382364 | type 4b pilus protein PilO2 | - |
| RIK67_RS00785 (RIK67_00785) | 25963..27639 | - | 1677 | WP_048210024 | PilN family type IVB pilus formation outer membrane protein | - |
| RIK67_RS00790 (RIK67_00790) | 27654..28094 | - | 441 | WP_284950950 | type IV pilus biogenesis protein PilM | - |
| RIK67_RS00795 (RIK67_00795) | 28091..28897 | - | 807 | WP_311382365 | TcpQ domain-containing protein | - |
Host bacterium
| ID | 16223 | GenBank | NZ_CP134669 |
| Plasmid name | 99202522619|unnamed2 | Incompatibility group | - |
| Plasmid size | 86517 bp | Coordinate of oriT [Strand] | 69251..69337 [-] |
| Host baterium | Enterobacter ludwigii strain 99202522619 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |