Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 115783
• Name: oriT_pWSM1274_2
• Organism: Rhizobium sp. WSM1274
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP104140 (197104..197132 [+], 29 nt)
• oriT length: 29 nt
• IRs (inverted repeats): 14..19, 24..29 (CGTCGC..GCGACG)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 29 nt

>oriT_pWSM1274_2
AGGGCGCAATATACGTCGCTGGCGCGACG

oriT secondary structure

Predicted by RNAfold.

Relaxase

traA_N2600_RS30720_pWSM1274_2

• ID: 10107
• GenBank: WP_260299698
• Name: traA_N2600_RS30720_pWSM1274_2
• UniProt ID: _
• Length: 1543 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 1543 a.a.
• Molecular weight: 170786.07 Da
• Isoelectric Point: 7.4067

>WP_260299698.1 Ti-type conjugative transfer relaxase TraA [Rhizobium sp. WSM1274]
MAIMFVRAQVIGRGGGRSIVSAAAYRHRTRMMDEQAGTSFSYRGGASELVHEELALPDQTPDWLRAAIDG
RTVAAASEALWNAVDAFEKRADAQLARELIIALPEELTRAENIALVREFVRDNLTSKGMVADWVFHDKDS
NPHIHLMTTLRPLTEEGFGRKKVPVTGEDGTPLRVVTPDRPNGRIVYKLWAGDKETMKAWKIAWAETANR
HLALAGHEIRLDGRSYAERGLDGIAQKHLGPEKAALARKGRELYFAPADLARRQEMADRLLADPALLLKQ
LGNERSTFDERDIAKALHRYVDDPADFANIRARLMASDDLIMLKPQQIDAETGKASEPAVFTTREILRIE
YQMARSAQVLSERRGFAVSSRHVAAAVRHVETGDPEKPFKLDAEQVDAIRHVTGDNGIAAVVGLAGAGKS
TLLAGARIAWEHDGRRVIGSALAGKAAEGLEDSSGIRSRTLASWELIWANGHETLHRGDVLVIDEAGMVS
SQQMARVLKIAEEAGTKVVLVGDAMQLQPIQAGAAFRAITERIGFAELAGVRRQREPWAREASRLFARGE
VEKGLDAYAQQGHLVEAATRDEIIGRIVSDWTDARRALVRETAEGANTTRLRGDELLVLAHTNQDVKRLN
EALRLVMTDEGALGAGRSFRTERGAREFAAGDRIIFLENARFLEPRAPRLGPQYVKNGMLGTVVSTGDKR
GDTLLSVRLDNGRDVVISENSYRNVDHGYAATIHKSQGVTVERTFVLATGMMDQHLTYVSMTRHRDRADL
YAAKDDFEARPEWGRKPRVDHAAGVTGALVETGQAKFRPNDEDADDSPYADVKADDGTLHRLWGVSLPKA
LEQGGVSQGDTVTLRKDGVEKVKVQVQIVDEQTGQKRFEEKEVDRNVWTAKQIETAEARLERIERESHRP
EVFKQLALRLSRSGAKTTTLDFESEAGYRAHADDFARRRGLDHLSLAAAGMEESLSRQWAWIAEKREQVA
ELWERASMALGFAIERERRVAYSEQRTEPQPVSEADVAGGRYLIAPTTVFARSVEEDARLAQLSSPAWKE
REAILQPLLEKIYRDPDAALSALNALSSDMSIEPRRLADDLAAAPDRLGRLRGSDLIVDGRAARDERIAA
ITALTDLLPLARAHATEFRRNAERFESREQQRRTHMSLPIPALSKTAMTRLAEIEAVRRQGGDDAYKTAF
ALAAEDRSFVQEVKAVSEALSARFGWSAFTSRADAVAERNMIERMPEDLASISGEKLIRLFEAVKRFADE
QHQVERRDRSKIVAAASADQGMEADKENAAVLPMLAAITEFKNPLDEEARSRLLSDPLYRQQRAALAAVA
STIWRDPADAVGKIEDLLAKGFAGERIAAAVTNDPAAYGALRGSDRLMDRMLAAGRERKVAVQAVPEAAA
RLRALGSAYVNVLDAERQAVAEERRRMAVAIPGLSKAAEEALMRLTAEARNNGRKRNAPAVSLDPTIRQE
FAAVSRALDERFGRNAIIRGEKDLIHRVPQMQRNAFEAMQEKLKVLQQAVRRESSEQIISERRQRAVSRG
HGL

Protein domains

Predicted by InterproScan.

• MobA/MobL family (MobA_MobL) (17-249)

• BID domain of Bartonella effector protein (Bep) (Bep_C_terminal) (1046-1127)

• BID domain of Bartonella effector protein (Bep) (Bep_C_terminal) (1312-1406)

• AAA domain (AAA_30) (391-577)

Protein structure

No available structure.

Host bacterium

• ID: 16216
• GenBank: NZ_CP104140
• Plasmid name: pWSM1274_2
• Incompatibility group: -
• Plasmid size: 423083 bp
• Coordinate of oriT [Strand]: 197104..197132 [+]
• Host baterium: Rhizobium sp. WSM1274

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: actP
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -