Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 115746 |
| Name | oriT_p22ES-84  |
| Organism | Enterobacter hormaechei strain 22ES |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CM008906 (15114..15219 [+], 106 nt) |
| oriT length | 106 nt |
| IRs (inverted repeats) | 30..35, 41..46 (CCGCCG..CGGCGG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 106 nt
>oriT_p22ES-84
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 10085 | GenBank | WP_050868819 |
| Name | traI_CS900_RS08225_p22ES-84 |
UniProt ID | A0A142BMF1 |
| Length | 659 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75234.98 Da Isoelectric Point: 10.0285
>WP_050868819.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPVYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSERDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPVYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSERDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A142BMF1 |
T4CP
| ID | 11778 | GenBank | WP_101429073 |
| Name | t4cp2_CS900_RS08410_p22ES-84 |
UniProt ID | _ |
| Length | 695 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 695 a.a. Molecular weight: 79491.86 Da Isoelectric Point: 4.8656
>WP_101429073.1 conjugal transfer protein TrbC [Enterobacter hormaechei]
MQQESVDSKKVIRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPKVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMHFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFADWLDGTDTDDVLYHQEDDLFPMIEM
MQQESVDSKKVIRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPKVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMHFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFADWLDGTDTDDVLYHQEDDLFPMIEM
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 18015..37118
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| CS900_RS08205 (CS900_08185) | 13099..13410 | + | 312 | WP_011091065 | hypothetical protein | - |
| CS900_RS08210 (CS900_08190) | 13543..14079 | + | 537 | WP_011091066 | hypothetical protein | - |
| CS900_RS08215 (CS900_08195) | 14580..14945 | - | 366 | WP_011091067 | hypothetical protein | - |
| CS900_RS27160 | 14960..15538 | + | 579 | WP_223811519 | plasmid mobilization protein MobA | - |
| CS900_RS08225 (CS900_08205) | 15525..17504 | + | 1980 | WP_050868819 | TraI/MobA(P) family conjugative relaxase | - |
| CS900_RS08230 (CS900_08210) | 17518..18018 | + | 501 | WP_004187320 | DotD/TraH family lipoprotein | - |
| CS900_RS08235 (CS900_08215) | 18015..18794 | + | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| CS900_RS08240 (CS900_08220) | 18805..19968 | + | 1164 | WP_004187313 | plasmid transfer ATPase TraJ | virB11 |
| CS900_RS08245 (CS900_08225) | 19958..20218 | + | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| CS900_RS26905 | 20243..23455 | + | 3213 | WP_011091070 | LPD7 domain-containing protein | - |
| CS900_RS08255 (CS900_08235) | 23421..23933 | + | 513 | WP_011091071 | hypothetical protein | traL |
| CS900_RS08260 (CS900_08240) | 23934..24146 | - | 213 | WP_305953721 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| CS900_RS08265 (CS900_08245) | 24118..24528 | - | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| CS900_RS08270 (CS900_08250) | 24596..25309 | + | 714 | WP_109914255 | DotI/IcmL family type IV secretion protein | traM |
| CS900_RS08275 (CS900_08255) | 25318..26469 | + | 1152 | WP_011091074 | DotH/IcmK family type IV secretion protein | traN |
| CS900_RS08280 (CS900_08260) | 26481..27830 | + | 1350 | WP_011091075 | conjugal transfer protein TraO | traO |
| CS900_RS08285 (CS900_08265) | 27842..28546 | + | 705 | WP_011091076 | conjugal transfer protein TraP | traP |
| CS900_RS08290 (CS900_08270) | 28570..29100 | + | 531 | WP_011091077 | conjugal transfer protein TraQ | traQ |
| CS900_RS08295 (CS900_08275) | 29117..29506 | + | 390 | WP_011091078 | DUF6750 family protein | traR |
| CS900_RS08300 (CS900_08280) | 29552..30046 | + | 495 | WP_011091080 | hypothetical protein | - |
| CS900_RS08305 (CS900_08285) | 30043..33093 | + | 3051 | WP_101429072 | conjugal transfer protein | traU |
| CS900_RS08310 (CS900_08290) | 33090..34298 | + | 1209 | WP_011091082 | conjugal transfer protein TraW | traW |
| CS900_RS08315 (CS900_08295) | 34295..34945 | + | 651 | WP_011091083 | hypothetical protein | - |
| CS900_RS08320 (CS900_08300) | 34938..37118 | + | 2181 | WP_004187492 | DotA/TraY family protein | traY |
| CS900_RS08325 (CS900_08305) | 37121..37774 | + | 654 | WP_011091084 | hypothetical protein | - |
| CS900_RS08330 (CS900_08310) | 37849..38079 | + | 231 | WP_011091085 | IncL/M type plasmid replication protein RepC | - |
| CS900_RS08335 (CS900_08315) | 38377..39432 | + | 1056 | WP_011091086 | plasmid replication initiator RepA | - |
| CS900_RS08340 (CS900_08320) | 40436..41296 | - | 861 | WP_000027057 | broad-spectrum class A beta-lactamase TEM-1 | - |
Host bacterium
| ID | 16179 | GenBank | NZ_CM008906 |
| Plasmid name | p22ES-84 | Incompatibility group | IncL/M |
| Plasmid size | 83956 bp | Coordinate of oriT [Strand] | 15114..15219 [+] |
| Host baterium | Enterobacter hormaechei strain 22ES |
Cargo genes
| Drug resistance gene | blaTEM-1B, aac(3)-IId, aac(6')-Ib-cr, blaOXA-1, blaCTX-M-3 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |