Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 115744
• Name: oriT_p22ES-287
• Organism: Enterobacter hormaechei strain 22ES
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CM008898 (13857..14111 [-], 255 nt)
• oriT length: 255 nt
• IRs (inverted repeats): 153..158, 160..165 (AAAAGT..ACTTTT) 122..130, 135..143 (TTAAGGCTT..AAGCCTTAA) 49..55, 60..66 (GAATTTT..AAAATTC)
• Location of nic site: 78..79
• Conserved sequence flanking the nic site: TTTGGTTAAA
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 255 nt

>oriT_p22ES-287
GGATTTAGGTTTTTTTTTAATCGCTTCACATTTCGTTAGCATGCGCAGGAATTTTTGATAAAATTCTGGTTAGTTTGGTTAAAAAGTGTTACAAGTAAGCGGTGTGGTTGAAGGGATAGATTTAAGGCTTATTCAAGCCTTAAGAAAATACTAAAAGTTACTTTTCACCCTACCGAACACCTAACAAAAAATCCATGTTGAAGATTTGAACAATTGTAATGGCGCAAGGACAATCAGCACATGTCAGAATCTGAT

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobH_CS900_RS02355_p22ES-287

• ID: 10083
• GenBank: WP_012477394
• Name: mobH_CS900_RS02355_p22ES-287
• UniProt ID: A0A2Z2E7B6
• Length: 1050 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 1050 a.a.
• Molecular weight: 118067.47 Da
• Isoelectric Point: 4.6347

>WP_012477394.1 MULTISPECIES: MobH family relaxase [Enterobacterales]
MMMNFRALYLCIKRILGIFSSQENDATSVMIEDISSLSPFAQILGDQKYTVPDHPNPEVLKFIEYPTRPT
GIQTFNEQSILSLYREKLHSISMMLAISDSDIRDDAYTFTNLVLKPLVEYVRWIHLLPASENHHHNGIGG
LLSHSLEVAILSLKNAHHSELRPIGYQDEEVVRRKVYLYAAFICGLVHDAGKVYDLDIVSLNLASPIIWT
PSSQSLLDWARENDVVEYEIHWRKRIHNQHNIWSSVFLERILNPVCLAFLDRVNKERVYSKMITALNVYT
DGNDFLSKCVRTADFYSTGTDLNVLRDPIMGLRSNDAAARAISTIKHNFTSININNYNAKPMHIIIVNGE
VYLNENAFLDFVLNDFELHKYNFPQGEAGKTVLVESLVQRGYVEPYDDERVVHYFIPGIYSENEISNIFR
NGIGKLEFYNLLKLRWIGLIFDSYKIPDSVPGLFSVNANKDFIYIDEQKTVTEYRRPVPGRDVITKITDT
VETAVLKVNDLGRSSASIDVDIHSKKNEGSSDDFEKKAESDNEIDNDTQIVKSEGEEAADPVIPDIEESE
DESAKDTESHVLVNQLHELLLSAPLSNDYIVCVDAVPYLNIDTTMALLPGLDEKAFSEEPYFQLTFREGS
LDGMWIVRDIDDLRLVQLGDNCAGFQLTYHEPRRPTTLKSLFNTSMYQALVINDESSVENSAPRPKQTLE
LPPPRVNAVEEHSGDVEYHGTDSASATGPLKTEAVEYEHYQHLFEKEDEEHEIIDYTDFSQLSVSRPEVG
SCATSSSVHNEKLLSEPSELPELNREQNADPQGTNERSMDVSVGQENSEPDTEGNCPPPAEVVYSQTEAA
ATSVMASEEPALPPVLEESNGEHAPTDAKGHHLSPALARLFAPTAPVEKQNPKRNRNKSSDKAEVQKPAS
PVSGHNLNSKVFASTESDQNGEFSLISEGDVTELEFVEIALVLHQILSKMEVAFKRKRKNRFMVSTPNTL
YLTQSCVEKFGSQLEAQDLFNKLPQYLVNSGAVINTKCHAFNMPTLLAASDRAKVDIERIINNLKEAGNL

Protein domains

Predicted by InterproScan.

• Putative helicase (TraI_2) (108-304)

Protein structure

• AlphaFold DB: A0A2Z2E7B6

T4CP

traD_CS900_RS02360_p22ES-287

• ID: 11775
• GenBank: WP_001284073
• Name: traD_CS900_RS02360_p22ES-287
• UniProt ID: _
• Length: 694 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 694 a.a.
• Molecular weight: 78123.91 Da
• Isoelectric Point: 8.0177

>WP_001284073.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Enterobacterales]
MSDSKRTNLHAQENFYRPILEYRSASILLICSVSMLYMGLSSDGLDIAPIVLFTSILLFLLCLYRCKTAA
PFLMAHWRVFKRHFMFVSLDSLRVINKSNFFSNERKYRQLVQDYQNKNKDIPERKSYFCDGFEWGPEHAD
RAYQIANLSSDKREIELPFVFNPIKRHFDAMARKMGGSNAIFAVERREPIFVTEDNWFGHTLITGNVGTG
KTVLQRLLSISMLHLGHVVVVIDPKNDAEWRESLMEEAKTLGLPFYKFHPGQPASSVCIDVCNTYTNVSD
LTSRLLSLVTVPGEVNPFVQYAKALVSNVISGLSYIEKKPSIYLIHKNMKSHMSIVNLTVKVMESCYARY
YGYDVWTEKVKYVANDTLPVRFKRLAEWFTAHFMNYEGSEQIDWLDTVSQLIDYSMSDPEHMAKMTAGIM
PVFDMLIEKPLNELLSPNPNSVSSREIVTSEGMFSTGGVLYISLDGLSNPDTAAAISQLIMSDLTSCAGS
RYNAQDGDMSANSRISIFVDEAHSAINNPMINLLAQGRAAKIALFICTQTISDFIAAASVETANRITGLC
NNYISLRVNDTPTQTLVVENFGKSAISTNMVTYTTGSETSLPHNNFSGSISERKQTTLEESIPKDLLGQV
PMFHIVARLQDGRKVVGQIPIAVAEKQMKPNTTLSEMLFKKAGKVTLRQNLDIKNLNKFLRKLH

Protein domains

Predicted by InterproScan.

• Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind) (406-609)

• AAA-like domain (AAA_10) (199-249)

Protein structure

No available structure.

T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 120246..129485

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CS900_RS02960 (CS900_02950)	115389..116105	-	717	WP_000838219	zinc finger-like domain-containing protein	-
CS900_RS02965 (CS900_02955)	116102..116302	-	201	WP_000594930	hypothetical protein	-
CS900_RS02970 (CS900_02960)	116322..117374	-	1053	WP_001065778	hypothetical protein	-
CS900_RS02975 (CS900_02965)	117851..118726	+	876	WP_000594611	RepB family plasmid replication initiator protein	-
CS900_RS02985 (CS900_02975)	119875..120228	+	354	WP_000423602	hypothetical protein	-
CS900_RS02990 (CS900_02980)	120246..120596	+	351	WP_001447770	type IV conjugative transfer system protein TraL	traL
CS900_RS02995 (CS900_02985)	120608..121396	+	789	WP_101429029	TraE/TraK family type IV conjugative transfer system protein	traE
CS900_RS03000 (CS900_02990)	121396..122598	+	1203	WP_242449837	type-F conjugative transfer system secretin TraK	traK
CS900_RS03005 (CS900_02995)	122668..123129	+	462	WP_000521242	plasmid transfer protein HtdO	-
CS900_RS03010 (CS900_03000)	123119..124474	+	1356	WP_000351841	IncHI-type conjugal transfer protein TrhB	traB
CS900_RS03015 (CS900_03005)	124482..124964	+	483	WP_000377632	hypothetical protein	-
CS900_RS03020 (CS900_03010)	125083..125835	+	753	WP_182911723	protein-disulfide isomerase HtdT	-
CS900_RS03025 (CS900_03015)	125845..126795	+	951	WP_001022588	IncHI-type conjugal transfer lipoprotein TrhV	traV
CS900_RS27315	126972..128084	+	1113	Protein_144	TraC family protein	-
CS900_RS27320	128634..129485	+	852	WP_276542696	hypothetical protein	virb4
CS900_RS26650	132034..132195	+	162	WP_001371949	hypothetical protein	-

Host bacterium

• ID: 16177
• GenBank: NZ_CM008898
• Plasmid name: p22ES-287
• Incompatibility group: IncHI2
• Plasmid size: 286650 bp
• Coordinate of oriT [Strand]: 13857..14111 [-]
• Host baterium: Enterobacter hormaechei strain 22ES

Cargo genes

• Drug resistance gene: tet(D), blaSHV-12, qnrA1, sul1, dfrA19, aph(3'')-Ib, aph(6)-Id, blaTEM-1B, catA2, mcr-9
• Virulence gene: -
• Metal resistance gene: terW, terZ, terA, terB, terC, terD, terE, pbrA, merE, merD, merA, merC, merP, merT, merR, pcoS, pcoE, ncrC, rcnR/yohL, arsC, arsB, arsR, arsH
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -