Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   115607
Name   oriT_pK92-KPC in_silico
Organism   Klebsiella michiganensis strain K92
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_OL828742 (90465..90514 [-], 50 nt)
oriT length   50 nt
IRs (inverted repeats)      7..14, 17..24  (GCAAAATT..AATTTTGC)
Location of nic site      33..34
Conserved sequence flanking the
  nic site    		  TGTGTGGTGA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 50 nt

>oriT_pK92-KPC
AAATCTGCAAAATTTTAATTTTGCGTAGTGTGTGGTGATTTTGTGGTGAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   9993 GenBank   WP_075606914
Name   traI_N1J62_RS00710_pK92-KPC insolico UniProt ID   A0A6M3HIK1
Length   1752 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1752 a.a.        Molecular weight: 190386.65 Da        Isoelectric Point: 5.5084

>WP_075606914.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Enterobacteriaceae]
MLSFSQVKSAGSAGNYYTEKDNYYVIGSMEERWQGKGAEALGLEGKVDKQVFTELLQGKLPDGSDLTRMQ
DGVNKHRPGYDLTFSAPKSVSMLAMLGGDKRLIDAHNRAVTVALNQVESLASTRVQKDGVSETVLTNNLI
IARFNHDTSRAQEPQIHTHSVVINATQNGDKWQTLGSDTVGKTGFSENILANRIAFGKIYQSTLRADVES
MGYKTVDAGKHGMWEMEGVPVESFSTRSQELREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMNT
LKETGFDIRGYREAADTRAKELASAPAAPVKTDVPDISDVVTKAIAGLSDRKVQFTYADLLARTVSQLEA
KPGVFEQARTGIDAAIEREQLIPLDREKGLFTSNIHVLDELSVKALSQEIQRQNHVSVTPDTAVVRQSPF
SDAVSVLAQDRPTMGIVSGQGGAAGQRERVAELTLMAREQGRDVHIIAADNRSREFLSADARLAGETVTG
KSALQDGTAFIPGGTLIVDQAEKMSLKETLSMLDGAMRHNVQVLLSDSGKRSGTGSALTVLKESGVNTYR
WQGGKQATADIISEPEKGARYSRLAQEFAASVREGQESVAQISGSREQGILNGLIRETLKSEGVLGEKDM
TVTALTPVWLDSKSRGVRDYYREGMVMERWDPETRQHDRFVIDRVTASSNMLTLRDKEGERLDMKVSAVD
SQWTLFRADKLTVAEGERLAVLGKIPDTRLKGGESVTVLKAEEGQLTVQRPGQKTTQSLSVGSSPFDGIK
VGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHIRLYSAQGTARTTEKLARHTAFSVVSEQ
LKARSGETDLDAAIASQKAGLHTPAEQVIHLSIPLLESEELTFTRPQLLATALETGGGKVPMTEIDATIQ
SQIQAGSLLNVPVAPGHGNDLLISRQTWDAEKSILTKVLEGKEAVAPLMDNVPGSLMTDLTSGQRASTRM
ILETTDRFTVVQGYAGVGKTTQFRAVTGAISLLPEATRPRVIGLGPTHRAVGEMQSAGVDAQTTASFLHD
TQLLQRNGQTPDFSNTLFLLDESSMVGLADTAKALSLIAAGGGRAVLSGDTDQLQSIAPGQPFRLMQQRS
AADVAIMKEIVRQVPELRPAVYSLIDRDVNRALATIEQVTPEQVPRKDGAWVPENSVVEFTPAQEEAIQK
ALNKGESVPAGQPTTLYEALVKDYADRTPEAQSQTLIITHLNDDRRVLNSLIHDARRDNGEVGREEVTLP
VLVTSNIRDGELRRLSTWTAHKGAVALVDNVYHRIGSVDKENQLITLMDGEGKERLISPREALAEGVTLY
REQEITVSQGDKMRFSKSDTERGYVANSVWEVKSVSGDSVTLSDGKSTRTLNPKADQAQQHIDLAYAITA
HGAQGASEPFAIALEGVAGGRERMASFESAYVGLSRMKQHVQVYTDNREGWIKAINNSPEKATAHDILEP
RNDRAVKTAGQLFSRARALDEAAAGRAALQQSGLARGQSPGKFISPGKKYPQPHVALPAFDKNGKDAGIW
LSPLTDNDGRLQVIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRIARDNPDSGVVVRLAGDDRP
WNPEAITGGRIWADPLPNPPINDTGTDIPLPPEVLAQRAAEEAQRREMERQTEQATREVSGEEKKAGEPG
ERIKEVIGDVIRGLERDRPGEEKTVLPDDPQTRRQEAAVQQVVSESLQRDRLQQMERDMVRDLNREKTLG
GD

  Protein domains


Predicted by InterproScan.

DNA helicase TraI (TraI_C)

(1432-1552)

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(577-628)

TrwC relaxase (TrwC)

(11-283)

AAA domain (AAA_30)

(970-1157)

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(634-713)


  Protein structure



No available structure.




Auxiliary protein

ID   5857 GenBank   WP_053390193
Name   WP_053390193_pK92-KPC insolico UniProt ID   A0A8G2A327
Length   130 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 130 a.a.        Molecular weight: 14883.89 Da        Isoelectric Point: 4.3779

>WP_053390193.1 MULTISPECIES: conjugal transfer relaxosome DNA-binding protein TraM [Enterobacteriaceae]
MAKVQAYVSDEVADKINAIVEKRRVEGAKDKDISFSSISTMLLELGLRVYEAQMERKESGFNQMAFNKAL
LESVIKTQFTVNKVLGIECLSPHVSGNPRWEWPGLIENIRDDVQEVMLRFFPDEESEDEE

  Protein domains


Predicted by InterproScan.

TraM protein, DNA-binding (Tra_M)

(1-125)


  Protein structure



No available structure.




T4CP

ID   11663 GenBank   WP_075606915
Name   traD_N1J62_RS00705_pK92-KPC insolico UniProt ID   _
Length   772 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 772 a.a.        Molecular weight: 86712.47 Da        Isoelectric Point: 5.0825

>WP_075606915.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacteriaceae]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFIIFWVLCGLILMYRLSWQTFVNGCVYWWCTTLAPMR
DIIRSQPVYTINYYGKSLEYNSEQILADKYTIWCGEQLWTSFVFAAIVSLTVCIVTFFVASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGVASDIKIGDLPILKHSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDIWRECLTLPDFDNVSNTLIPMGTKEDPFWQG
SGRTIFSEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLKNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDQPNGWLFISSNADTHASLKPVISMWLSIAIRGLLGMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKSRPKIAEGFILRNLDTRTDARLDTLLEAREAESSHARTLFTPDAPAPELAEKDEKVGNPPEQSQPAE
SSGTPATVKVPSTAKTPEADESGQSPEVSNPAALLTKVVTVPLTRPRPAAATGTAAVASATDVRATPAGG
TEQALETQPAEQGQDMLPPGVNEYGEIDDMHAWDEWQSGEQTQRDMQRREEVNINHSHRRDEQDDIEIGG
NF

  Protein domains


Predicted by InterproScan.

F sex factor protein N terminal (TraD_N)

(32-128)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(172-560)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 92933..117651

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N1J62_RS00530 88871..89338 - 468 WP_053390247 hypothetical protein -
N1J62_RS00535 89333..89875 + 543 WP_053390246 antirestriction protein -
N1J62_RS00540 89898..90320 - 423 WP_077268549 transglycosylase SLT domain-containing protein -
N1J62_RS00545 90826..91218 + 393 WP_053390193 conjugal transfer relaxosome DNA-binding protein TraM -
N1J62_RS00550 91457..92131 + 675 WP_075606928 hypothetical protein -
N1J62_RS00555 92325..92486 + 162 WP_172686697 TraY domain-containing protein -
N1J62_RS00560 92551..92919 + 369 WP_053390195 type IV conjugative transfer system pilin TraA -
N1J62_RS00565 92933..93238 + 306 WP_053390196 type IV conjugative transfer system protein TraL traL
N1J62_RS00570 93258..93824 + 567 WP_053390197 type IV conjugative transfer system protein TraE traE
N1J62_RS00575 93811..94545 + 735 WP_053390198 type-F conjugative transfer system secretin TraK traK
N1J62_RS00580 94545..95966 + 1422 WP_075606927 F-type conjugal transfer pilus assembly protein TraB traB
N1J62_RS00585 95959..96555 + 597 WP_075606926 conjugal transfer pilus-stabilizing protein TraP -
N1J62_RS00590 96578..97147 + 570 WP_075606925 type IV conjugative transfer system lipoprotein TraV traV
N1J62_RS00595 97259..97537 + 279 WP_053390295 hypothetical protein -
N1J62_RS00600 97544..97732 + 189 WP_053390294 hypothetical protein -
N1J62_RS00605 97816..98217 + 402 WP_075606924 hypothetical protein -
N1J62_RS00610 98214..98513 + 300 WP_053390307 hypothetical protein -
N1J62_RS00615 98672..98983 + 312 WP_227524648 hypothetical protein -
N1J62_RS00620 99076..101715 + 2640 WP_075606922 type IV secretion system protein TraC virb4
N1J62_RS00625 101715..102104 + 390 WP_060415481 type-F conjugative transfer system protein TrbI -
N1J62_RS00630 102101..102727 + 627 WP_060415482 type-F conjugative transfer system protein TraW traW
N1J62_RS00635 102771..103730 + 960 WP_088912317 conjugal transfer pilus assembly protein TraU traU
N1J62_RS00640 103743..104369 + 627 WP_075606921 type-F conjugative transfer system pilin assembly protein TrbC trbC
N1J62_RS00645 104366..106321 + 1956 WP_075606920 type-F conjugative transfer system mating-pair stabilization protein TraN traN
N1J62_RS00650 106353..106613 + 261 WP_053390216 hypothetical protein -
N1J62_RS00655 106606..107217 + 612 WP_053390217 hypothetical protein -
N1J62_RS00660 107207..107449 + 243 WP_082226157 conjugal transfer protein TrbE -
N1J62_RS00665 107495..107821 + 327 WP_053390219 hypothetical protein -
N1J62_RS00670 107842..108594 + 753 WP_053390220 type-F conjugative transfer system pilin assembly protein TraF traF
N1J62_RS00675 108605..108841 + 237 WP_053390221 type-F conjugative transfer system pilin chaperone TraQ -
N1J62_RS00680 108816..109376 + 561 WP_039698500 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
N1J62_RS00685 109363..110733 + 1371 WP_075606918 conjugal transfer pilus assembly protein TraH traH
N1J62_RS00690 110733..113588 + 2856 WP_075606917 conjugal transfer mating-pair stabilization protein TraG traG
N1J62_RS00695 113653..114276 + 624 WP_258237686 hypothetical protein -
N1J62_RS00700 114522..115214 + 693 WP_202395549 hypothetical protein -
N1J62_RS00705 115333..117651 + 2319 WP_075606915 type IV conjugative transfer system coupling protein TraD virb4

Host bacterium

ID   16040 GenBank   NZ_OL828742
Plasmid name   pK92-KPC Incompatibility group   IncFII
Plasmid size   128222 bp Coordinate of oriT [Strand]   90465..90514 [-]
Host baterium   Klebsiella michiganensis strain K92

Cargo genes

Drug resistance gene   blaKPC-2
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -