Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 115551 |
| Name | oriT_pMB5971_1  |
| Organism | Klebsiella aerogenes strain 722 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP103640 (152105..152154 [+], 50 nt) |
| oriT length | 50 nt |
| IRs (inverted repeats) | 7..14, 17..24 (GCAAAATT..AATTTTGC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 50 nt
>oriT_pMB5971_1
AAATCTGCAAAATTTTAATTTTGCGTAGTGTGTGGTCATTTTGTGGTGAG
AAATCTGCAAAATTTTAATTTTGCGTAGTGTGTGGTCATTTTGTGGTGAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 11616 | GenBank | WP_023313941 |
| Name | traD_M5T48_RS25920_pMB5971_1 |
UniProt ID | _ |
| Length | 769 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 769 a.a. Molecular weight: 85666.78 Da Isoelectric Point: 5.2499
>WP_023313941.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacterales]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPASGTADTNSHAGEQPEPVSQPA
PAEVTVSPAPVKAPATTKMPAAEPSARTAEPPVLRVTTVPLIKPKAAAAAAAAATASSAGTPAAAAGGTE
QELAQQSAEQGQDMLPAGMNKDGVIEDMQAYDAWADEQTLRDMQRREEVNINHSHRHDEQDDVEIGGNF
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPASGTADTNSHAGEQPEPVSQPA
PAEVTVSPAPVKAPATTKMPAAEPSARTAEPPVLRVTTVPLIKPKAAAAAAAAATASSAGTPAAAAGGTE
QELAQQSAEQGQDMLPAGMNKDGVIEDMQAYDAWADEQTLRDMQRREEVNINHSHRHDEQDDVEIGGNF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 121761..152712
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| M5T48_RS25920 (M5T48_25920) | 121761..124070 | - | 2310 | WP_023313941 | type IV conjugative transfer system coupling protein TraD | virb4 |
| M5T48_RS25925 (M5T48_25925) | 124197..124886 | - | 690 | WP_013023831 | hypothetical protein | - |
| M5T48_RS25930 (M5T48_25930) | 125079..125513 | - | 435 | Protein_135 | complement resistance protein TraT | - |
| M5T48_RS25935 (M5T48_25935) | 125528..126496 | + | 969 | WP_015958259 | IS5 family transposase | - |
| M5T48_RS25940 (M5T48_25940) | 126553..126874 | - | 322 | Protein_137 | complement resistance protein TraT | - |
| M5T48_RS25945 (M5T48_25945) | 127063..127590 | - | 528 | WP_032409657 | conjugal transfer protein TraS | - |
| M5T48_RS25950 (M5T48_25950) | 127596..130445 | - | 2850 | WP_023287129 | conjugal transfer mating-pair stabilization protein TraG | traG |
| M5T48_RS25955 (M5T48_25955) | 130445..131824 | - | 1380 | WP_072159474 | conjugal transfer pilus assembly protein TraH | traH |
| M5T48_RS25960 (M5T48_25960) | 131802..132245 | - | 444 | WP_023287131 | F-type conjugal transfer protein TrbF | - |
| M5T48_RS25965 (M5T48_25965) | 132291..132848 | - | 558 | WP_013214031 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
| M5T48_RS25970 (M5T48_25970) | 132820..133059 | - | 240 | WP_004144400 | type-F conjugative transfer system pilin chaperone TraQ | - |
| M5T48_RS25975 (M5T48_25975) | 133070..133822 | - | 753 | WP_004152677 | type-F conjugative transfer system pilin assembly protein TraF | traF |
| M5T48_RS25980 (M5T48_25980) | 133843..134169 | - | 327 | WP_004152676 | hypothetical protein | - |
| M5T48_RS25985 (M5T48_25985) | 134182..134430 | - | 249 | WP_013214029 | hypothetical protein | - |
| M5T48_RS25990 (M5T48_25990) | 134408..134662 | - | 255 | WP_023287133 | conjugal transfer protein TrbE | - |
| M5T48_RS25995 (M5T48_25995) | 134694..136649 | - | 1956 | WP_023287134 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
| M5T48_RS26000 (M5T48_26000) | 136708..137355 | - | 648 | WP_064757794 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
| M5T48_RS26005 (M5T48_26005) | 137631..138620 | - | 990 | WP_032439620 | conjugal transfer pilus assembly protein TraU | traU |
| M5T48_RS26010 (M5T48_26010) | 138617..139018 | - | 402 | WP_016831047 | hypothetical protein | - |
| M5T48_RS26015 (M5T48_26015) | 139053..139688 | - | 636 | WP_020325113 | type-F conjugative transfer system protein TraW | traW |
| M5T48_RS26020 (M5T48_26020) | 139688..140077 | - | 390 | WP_004167468 | type-F conjugative transfer system protein TrbI | - |
| M5T48_RS26025 (M5T48_26025) | 140077..142716 | - | 2640 | WP_032448278 | type IV secretion system protein TraC | virb4 |
| M5T48_RS26030 (M5T48_26030) | 142788..143186 | - | 399 | WP_072159473 | hypothetical protein | - |
| M5T48_RS26035 (M5T48_26035) | 143194..143583 | - | 390 | WP_065799586 | hypothetical protein | - |
| M5T48_RS26040 (M5T48_26040) | 143626..144030 | - | 405 | WP_016831042 | hypothetical protein | - |
| M5T48_RS26045 (M5T48_26045) | 144097..144408 | - | 312 | WP_014386200 | hypothetical protein | - |
| M5T48_RS26050 (M5T48_26050) | 144409..144627 | - | 219 | WP_014386199 | hypothetical protein | - |
| M5T48_RS26055 (M5T48_26055) | 144651..144941 | - | 291 | WP_014386198 | hypothetical protein | - |
| M5T48_RS26060 (M5T48_26060) | 144946..145356 | - | 411 | WP_014386197 | hypothetical protein | - |
| M5T48_RS26065 (M5T48_26065) | 145487..146071 | - | 585 | WP_014386196 | type IV conjugative transfer system lipoprotein TraV | traV |
| M5T48_RS26070 (M5T48_26070) | 146118..146690 | - | 573 | WP_014386195 | conjugal transfer pilus-stabilizing protein TraP | - |
| M5T48_RS26075 (M5T48_26075) | 146683..148107 | - | 1425 | WP_014386194 | F-type conjugal transfer pilus assembly protein TraB | traB |
| M5T48_RS26080 (M5T48_26080) | 148107..148847 | - | 741 | WP_014386193 | type-F conjugative transfer system secretin TraK | traK |
| M5T48_RS26085 (M5T48_26085) | 148834..149400 | - | 567 | WP_004152602 | type IV conjugative transfer system protein TraE | traE |
| M5T48_RS26090 (M5T48_26090) | 149420..149725 | - | 306 | WP_049110928 | type IV conjugative transfer system protein TraL | traL |
| M5T48_RS26095 (M5T48_26095) | 149739..150107 | - | 369 | WP_004194426 | type IV conjugative transfer system pilin TraA | - |
| M5T48_RS26100 (M5T48_26100) | 150169..150375 | - | 207 | WP_171773970 | TraY domain-containing protein | - |
| M5T48_RS26105 (M5T48_26105) | 150508..151227 | - | 720 | WP_014386192 | conjugal transfer protein | - |
| M5T48_RS26110 (M5T48_26110) | 151420..151836 | - | 417 | WP_072145360 | conjugal transfer relaxosome DNA-binding protein TraM | - |
| M5T48_RS26115 (M5T48_26115) | 152227..152712 | + | 486 | WP_004178063 | transglycosylase SLT domain-containing protein | virB1 |
| M5T48_RS26120 (M5T48_26120) | 152745..153074 | - | 330 | WP_014386191 | DUF5983 family protein | - |
| M5T48_RS26125 (M5T48_26125) | 153107..153928 | - | 822 | WP_004182076 | DUF932 domain-containing protein | - |
| M5T48_RS26130 (M5T48_26130) | 154761..155174 | - | 414 | WP_013023817 | type II toxin-antitoxin system HigA family antitoxin | - |
| M5T48_RS26135 (M5T48_26135) | 155175..155453 | - | 279 | WP_004152721 | helix-turn-helix transcriptional regulator | - |
| M5T48_RS26140 (M5T48_26140) | 155443..155763 | - | 321 | WP_004152720 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| M5T48_RS26145 (M5T48_26145) | 155844..156068 | - | 225 | WP_014386189 | hypothetical protein | - |
| M5T48_RS26150 (M5T48_26150) | 156079..156291 | - | 213 | WP_014386188 | hypothetical protein | - |
| M5T48_RS26155 (M5T48_26155) | 156353..156679 | - | 327 | WP_014386187 | hypothetical protein | - |
Host bacterium
| ID | 15984 | GenBank | NZ_CP103640 |
| Plasmid name | pMB5971_1 | Incompatibility group | IncFIB |
| Plasmid size | 170849 bp | Coordinate of oriT [Strand] | 152105..152154 [+] |
| Host baterium | Klebsiella aerogenes strain 722 |
Cargo genes
| Drug resistance gene | sul2, aph(3'')-Ib, aph(6)-Id, blaTEM-1B, blaCTX-M-15, dfrA14, blaOXA-1, aac(6')-Ib-cr, tet(A), qnrB1, aac(3)-IIa |
| Virulence gene | - |
| Metal resistance gene | silE, silS, silR, silC, silF, silB, silA, silP, pcoA, pcoB, pcoC, pcoD, pcoR, pcoS, pcoE, arsB, arsC, arsH |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |