Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 115540 |
| Name | oriT_pA10290_P3  |
| Organism | Enterococcus faecium strain A10290 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP059758 (40455..40646 [-], 192 nt) |
| oriT length | 192 nt |
| IRs (inverted repeats) | 106..112, 122..128 (ATTTTTT..AAAAAAT) 107..113, 120..126 (TTTTTTG..CAAAAAA) 41..49, 52..60 (CACCTTCCT..AGGAAGGTG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 192 nt
>oriT_pA10290_P3
CCAAAGAATTAATGCAAAGAGCATAAGGGAAAACTGATAGCACCTTCCTAAAGGAAGGTGGCTAAGTCGGCTGTGCCAACTGGTTTTCTTACAAAACAACCTTATATTTTTTGATATCACAAAAAAATCCATTTTGGACCTATTTTCGGTCATAATATAGTACCTACTTTTGGTCATAGTTTCGTCCGTAGT
CCAAAGAATTAATGCAAAGAGCATAAGGGAAAACTGATAGCACCTTCCTAAAGGAAGGTGGCTAAGTCGGCTGTGCCAACTGGTTTTCTTACAAAACAACCTTATATTTTTTGATATCACAAAAAAATCCATTTTGGACCTATTTTCGGTCATAATATAGTACCTACTTTTGGTCATAGTTTCGTCCGTAGT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 9953 | GenBank | WP_011377372 |
| Name | mobP2_HI839_RS15490_pA10290_P3 |
UniProt ID | Q33E54 |
| Length | 506 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 506 a.a. Molecular weight: 60652.14 Da Isoelectric Point: 9.4204
>WP_011377372.1 MULTISPECIES: MobP2 family relaxase [Enterococcus]
MTEHIFNRKSASIILKAKFETGKNKKKNHMVELQKFVDYISRQEAIRQDKLDHDYSEDELNELARIEKAL
EKIEQETDQPVIKDLREMDKYIDYMTRKKAILENVDNEIVNGAFSNTKRYITKKDISKIKESVIEAKNNG
SVMFQDVISFDNDFLVREGYYNPETNELNENILYEATKSMMGKMQEKEELVDPFWFATIHRNTEHIHIHV
TAMERKNTREIMEYDGVLQARGKRKQSTLDDMIFKFGSKILDRTNEFEKISKLRKEVPLELKQSVKDSLI
QLYVENNSRYDKELVKYLKELKKEIPSTTRGYNELPEETKEKIDEVTNYMTKDNPKRKEYDRMTKEIDEL
YQTTYGKRYNPQEYYLNRKKDFNARMGNAVVGQIKLLKQSEEKNLKSNELKNLLDGKKLNVKFDREKPEF
KTKYAKQSYDDFQKRLEERQLAREKNEVHFKKISEKFKDKKLIRKIDRVINDDLNMYRAKNDYEVAQRMI
EQEKMRQAREFEIGMN
MTEHIFNRKSASIILKAKFETGKNKKKNHMVELQKFVDYISRQEAIRQDKLDHDYSEDELNELARIEKAL
EKIEQETDQPVIKDLREMDKYIDYMTRKKAILENVDNEIVNGAFSNTKRYITKKDISKIKESVIEAKNNG
SVMFQDVISFDNDFLVREGYYNPETNELNENILYEATKSMMGKMQEKEELVDPFWFATIHRNTEHIHIHV
TAMERKNTREIMEYDGVLQARGKRKQSTLDDMIFKFGSKILDRTNEFEKISKLRKEVPLELKQSVKDSLI
QLYVENNSRYDKELVKYLKELKKEIPSTTRGYNELPEETKEKIDEVTNYMTKDNPKRKEYDRMTKEIDEL
YQTTYGKRYNPQEYYLNRKKDFNARMGNAVVGQIKLLKQSEEKNLKSNELKNLLDGKKLNVKFDREKPEF
KTKYAKQSYDDFQKRLEERQLAREKNEVHFKKISEKFKDKKLIRKIDRVINDDLNMYRAKNDYEVAQRMI
EQEKMRQAREFEIGMN
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | Q33E54 |
T4CP
| ID | 11606 | GenBank | WP_085803923 |
| Name | t4cp2_HI839_RS15420_pA10290_P3 |
UniProt ID | _ |
| Length | 952 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 952 a.a. Molecular weight: 108283.20 Da Isoelectric Point: 7.6503
>WP_085803923.1 VirD4-like conjugal transfer protein, CD1115 family [Enterococcus faecium]
MKLVQNNEVKEIKNGFVKNSNKILGKLKKVGSFKKNKYHTGKIEGEDLPKSLQDKLNVPLVFIGVFLGSI
VFLAITYLLNFLKALLVAIGQSESGLLGVEFDPKFSLAYLKPLGDTKQYFMCAVVAVIGVAYLIYGKLNH
ESEKNLVYGQKGDSRLSTIKEIKRDYHEIPEKTEVYDGIGGVPISHYQDKYYIDRDTVNTVVLGASRSGK
GETFVVPLLDNLSRAKIQSNMVVNDPKGELFSASKETLEKRGYRVLVLNIDDPLESMSFNPLQLVIDSWA
NKDYHEASKRANTLTSMLFASGMSTDNEFFYKSAKSAVNAIILTIVEHCFNNDCIEKITMYNVAQMLNEL
GSLFYTDPKTGKEKNALDEYFNTLPQGNQAKIQYGSTSFAGDKAKGSILSTASQGIEMFTSDLFGKLTSK
MSIDLKEIGFPKSIRFKVNTNLVGKRISISFLRKENDVIRLIKKYRVKVKALGLCVLNFNEYLQDGDMLD
IRYEEEKSRAWYRIEFPKKQANSHDTSLVTKKFKSGKNYSDLEISTLRLKYTDQPTAVFMVIPDYDPSNH
VLASIFISQLYTELASNCKQTPDKKCFRRVHFLLDEFGNMPAIDNMDGIMTVCLGRNMLFDLVIQSYSQL
ETRYDKAFKTIKENCQNHILIMSNDEETIEEISKKCGHKTTINRSASSKHLDTDSSVTSSAEQERVITVE
RASQLIEGEQIILRNLHRQDTKRNKIRPFPIFNTKETNMPYRYQFLAEDFDTQRDINEIDIACEHINLSL
QDNQIPYARFIKDLKTRLEYSIINNIPISEEDYQDYLNLSGSQQTINEEELLKLVNTEVKSQDMEGQVLS
EQEMKINIYLKESQKEAIELVKVINGRLADENSADMVIKTINSQILDTPFIQSLINDSIAIHNVQESKDQ
IMILEQQLANAYELAVFKTKNSILKNKIIKLKNAMTKLAKAI
MKLVQNNEVKEIKNGFVKNSNKILGKLKKVGSFKKNKYHTGKIEGEDLPKSLQDKLNVPLVFIGVFLGSI
VFLAITYLLNFLKALLVAIGQSESGLLGVEFDPKFSLAYLKPLGDTKQYFMCAVVAVIGVAYLIYGKLNH
ESEKNLVYGQKGDSRLSTIKEIKRDYHEIPEKTEVYDGIGGVPISHYQDKYYIDRDTVNTVVLGASRSGK
GETFVVPLLDNLSRAKIQSNMVVNDPKGELFSASKETLEKRGYRVLVLNIDDPLESMSFNPLQLVIDSWA
NKDYHEASKRANTLTSMLFASGMSTDNEFFYKSAKSAVNAIILTIVEHCFNNDCIEKITMYNVAQMLNEL
GSLFYTDPKTGKEKNALDEYFNTLPQGNQAKIQYGSTSFAGDKAKGSILSTASQGIEMFTSDLFGKLTSK
MSIDLKEIGFPKSIRFKVNTNLVGKRISISFLRKENDVIRLIKKYRVKVKALGLCVLNFNEYLQDGDMLD
IRYEEEKSRAWYRIEFPKKQANSHDTSLVTKKFKSGKNYSDLEISTLRLKYTDQPTAVFMVIPDYDPSNH
VLASIFISQLYTELASNCKQTPDKKCFRRVHFLLDEFGNMPAIDNMDGIMTVCLGRNMLFDLVIQSYSQL
ETRYDKAFKTIKENCQNHILIMSNDEETIEEISKKCGHKTTINRSASSKHLDTDSSVTSSAEQERVITVE
RASQLIEGEQIILRNLHRQDTKRNKIRPFPIFNTKETNMPYRYQFLAEDFDTQRDINEIDIACEHINLSL
QDNQIPYARFIKDLKTRLEYSIINNIPISEEDYQDYLNLSGSQQTINEEELLKLVNTEVKSQDMEGQVLS
EQEMKINIYLKESQKEAIELVKVINGRLADENSADMVIKTINSQILDTPFIQSLINDSIAIHNVQESKDQ
IMILEQQLANAYELAVFKTKNSILKNKIIKLKNAMTKLAKAI
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 18882..38544
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HI839_RS15355 (HI839_015350) | 14163..17792 | + | 3630 | WP_002311844 | collagen binding domain-containing protein | - |
| HI839_RS15360 (HI839_015355) | 17852..18214 | + | 363 | WP_002311845 | hypothetical protein | - |
| HI839_RS15365 (HI839_015360) | 18228..18380 | + | 153 | WP_002311846 | hypothetical protein | - |
| HI839_RS15370 (HI839_015365) | 18448..18870 | + | 423 | WP_002311847 | hypothetical protein | - |
| HI839_RS15375 (HI839_015370) | 18882..20297 | + | 1416 | WP_002311848 | CpaF/VirB11 family protein | virB11 |
| HI839_RS15380 (HI839_015375) | 20314..21129 | + | 816 | WP_002313315 | hypothetical protein | - |
| HI839_RS15385 (HI839_015380) | 21129..21926 | + | 798 | WP_002311850 | hypothetical protein | - |
| HI839_RS15390 (HI839_015385) | 21944..22306 | + | 363 | WP_002311851 | DUF4320 family protein | - |
| HI839_RS15395 (HI839_015390) | 22320..22466 | + | 147 | WP_002326866 | hypothetical protein | - |
| HI839_RS15400 (HI839_015395) | 22478..22699 | + | 222 | WP_002326867 | hypothetical protein | - |
| HI839_RS15405 (HI839_015400) | 22718..22993 | + | 276 | WP_002311852 | TrbC/VirB2 family protein | virB2 |
| HI839_RS15410 (HI839_015405) | 23102..24928 | + | 1827 | WP_002311853 | hypothetical protein | - |
| HI839_RS15415 (HI839_015410) | 24941..25657 | + | 717 | WP_002311854 | hypothetical protein | - |
| HI839_RS15420 (HI839_015415) | 25670..28528 | + | 2859 | WP_085803923 | VirD4-like conjugal transfer protein, CD1115 family | - |
| HI839_RS15425 (HI839_015420) | 28546..31215 | + | 2670 | WP_259395580 | pLS20_p028 family conjugation system transmembrane protein | - |
| HI839_RS15430 (HI839_015425) | 31239..31574 | + | 336 | WP_002329553 | DUF5592 family protein | - |
| HI839_RS15435 (HI839_015430) | 31571..32188 | + | 618 | WP_002329552 | hypothetical protein | virb4 |
| HI839_RS15440 (HI839_015435) | 32205..32672 | + | 468 | WP_002329551 | hypothetical protein | - |
| HI839_RS15445 (HI839_015440) | 32688..34313 | + | 1626 | WP_010729563 | VirB4 family type IV secretion system protein | virb4 |
| HI839_RS15450 (HI839_015445) | 35001..36878 | + | 1878 | WP_002305177 | group II intron reverse transcriptase/maturase | - |
| HI839_RS15455 (HI839_015450) | 37066..37386 | + | 321 | WP_010729568 | hypothetical protein | virb4 |
| HI839_RS15460 (HI839_015455) | 37408..38544 | + | 1137 | WP_002311861 | bifunctional lytic transglycosylase/C40 family peptidase | orf14 |
| HI839_RS15465 (HI839_015460) | 38558..39208 | + | 651 | WP_111249171 | cell division protein FtsL | - |
| HI839_RS15470 (HI839_015465) | 39222..40118 | + | 897 | WP_002311863 | hypothetical protein | - |
| HI839_RS15475 (HI839_015470) | 40154..40384 | + | 231 | WP_002311864 | hypothetical protein | - |
| HI839_RS15480 (HI839_015475) | 40735..41031 | + | 297 | WP_010776936 | hypothetical protein | - |
| HI839_RS15485 (HI839_015480) | 41028..41495 | + | 468 | WP_111249168 | hypothetical protein | - |
| HI839_RS15490 (HI839_015485) | 41578..43098 | + | 1521 | WP_011377372 | MobP2 family relaxase | - |
| HI839_RS15495 (HI839_015490) | 43120..43449 | + | 330 | WP_002311869 | hypothetical protein | - |
Host bacterium
| ID | 15973 | GenBank | NZ_CP059758 |
| Plasmid name | pA10290_P3 | Incompatibility group | - |
| Plasmid size | 54357 bp | Coordinate of oriT [Strand] | 40455..40646 [-] |
| Host baterium | Enterococcus faecium strain A10290 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIA1 |