Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   115514
Name   oriT_pIBB477e in_silico
Organism   Lactococcus cremoris subsp. cremoris IBB477
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CM007358 (8826..8962 [+], 137 nt)
oriT length   137 nt
IRs (inverted repeats)     _
Location of nic site      104..105
Conserved sequence flanking the
  nic site    		  GCTTGCAGTA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 137 nt

>oriT_pIBB477e
CGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTGGCCTCTTTTCAAGCCACATTGTCATACAAGAACGAAGTGATTTGTATGACAATGTGATAGCTTGCAGTATTTATGGTTTTATATGGTCTATTTTGTT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   9937 GenBank   WP_075070814
Name   Relaxase_AJ89_RS14645_pIBB477e insolico UniProt ID   A0A1E7G061
Length   410 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 410 a.a.        Molecular weight: 47727.39 Da        Isoelectric Point: 9.0605

>WP_075070814.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Lactococcus]
MATIAKISNGASAASALNYALGQDRPMHEKTEQWLQAHQLERPVELTNCRAVAVGGTNGIDPFIAKEQFD
VVRQLHNQTKESNQVLRITQSFALDELNPKVQKDWQKANDLGVELAENLYPNHQSAVYTHLDGKNHVLHN
HIIVNKVNLETGKKLREQKGESVQRAREMNDRLASRENWHILEPPKERQTETEKELIAKNEYSYMDDLRE
RINKSLQDVSVSSYETFKERLSDNGVILSERGQTFSYAFLDANNKQRRARETRLGSDFGRETILHELENR
ARQNEFRAVEQREPAITPLERDTQQRESEIVSLEQAIEPRKSEALKRESKINRFIDTIKQFAGRVPELTQ
RVTRKLKQTKEKILDDFERRFSKDMKNYEQEQQKSLEKQANRDVQSEKKPTKDHDRGMSR

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(9-280)


  Protein structure


Source ID Structure
AlphaFold DB A0A1E7G061


Host bacterium

ID   15947 GenBank   NZ_CM007358
Plasmid name   pIBB477e Incompatibility group   -
Plasmid size   11987 bp Coordinate of oriT [Strand]   8826..8962 [+]
Host baterium   Lactococcus cremoris subsp. cremoris IBB477

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -