Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 115513 |
| Name | oriT_pIBB477d  |
| Organism | Lactococcus cremoris subsp. cremoris IBB477 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CM007357 (7163..7299 [-], 137 nt) |
| oriT length | 137 nt |
| IRs (inverted repeats) | 21..28, 42..49 (AAAAGGGG..CCCCTTTT) 25..30, 39..44 (GGGGAA..TTCCCC) |
| Location of nic site | 104..105 |
| Conserved sequence flanking the nic site |
GCTTGCAGTA |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 137 nt
>oriT_pIBB477d
CGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGCAACATTGTAATACAAGAACGAAGTGATTTGTATTACAATGTGATAGCTTGCAGTATTTATGGGTTTATATTTGCTATTTTGTT
CGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGCAACATTGTAATACAAGAACGAAGTGATTTGTATTACAATGTGATAGCTTGCAGTATTTATGGGTTTATATTTGCTATTTTGTT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 9936 | GenBank | WP_075070803 |
| Name | Relaxase_AJ89_RS14490_pIBB477d |
UniProt ID | A0A1E7G0M9 |
| Length | 505 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 505 a.a. Molecular weight: 59285.09 Da Isoelectric Point: 7.0085
>WP_075070803.1 relaxase/mobilization nuclease domain-containing protein [Lactococcus cremoris]
MTVIYMPKQSNGTVHSAKDLKQLIDYVMNSEKTNDFEYVSGQNILDIHSTCDEMLATRTMAIALKNKPQK
NERFGYHFVQSFSPDDHLTPEQVHEIGCKTMKEYLGSSAEFIIATHTDKPHLHNHIVLNATDPLTLNKFQ
QNKNDLERLKEISDRISKEYGCKIIVRPEQKLGNSHKNYLVYLAQISYRKEIKNKLEFLMNHSHTWEDFK
EKASALNLKVDDTKKYTTYLLEGSEQTKKIRDRSLKNDKFLKENLKERIEKNTIGYSVEEVVKLWNDKES
IQEKNQEKEISILVEDWQIEKETEIFLYVTIDTALDKEATIKIPARCVDKLENGDYQVFIKNKDRFSYLD
KKSPANNKIMYGTTVARNLQRQSGNVPLYSDNANIKLKQVFHEFDFLISQGLGFDRSFETIGKDLKATYQ
ETEKQLDKLDTKIIEYVEQNKSLDYVDTTIRDTIKNLKLERDDLRDTLYKVDKNIQYYQKSEQRLEAYQK
NQSPKHKARDDDFEI
MTVIYMPKQSNGTVHSAKDLKQLIDYVMNSEKTNDFEYVSGQNILDIHSTCDEMLATRTMAIALKNKPQK
NERFGYHFVQSFSPDDHLTPEQVHEIGCKTMKEYLGSSAEFIIATHTDKPHLHNHIVLNATDPLTLNKFQ
QNKNDLERLKEISDRISKEYGCKIIVRPEQKLGNSHKNYLVYLAQISYRKEIKNKLEFLMNHSHTWEDFK
EKASALNLKVDDTKKYTTYLLEGSEQTKKIRDRSLKNDKFLKENLKERIEKNTIGYSVEEVVKLWNDKES
IQEKNQEKEISILVEDWQIEKETEIFLYVTIDTALDKEATIKIPARCVDKLENGDYQVFIKNKDRFSYLD
KKSPANNKIMYGTTVARNLQRQSGNVPLYSDNANIKLKQVFHEFDFLISQGLGFDRSFETIGKDLKATYQ
ETEKQLDKLDTKIIEYVEQNKSLDYVDTTIRDTIKNLKLERDDLRDTLYKVDKNIQYYQKSEQRLEAYQK
NQSPKHKARDDDFEI
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A1E7G0M9 |
Host bacterium
| ID | 15946 | GenBank | NZ_CM007357 |
| Plasmid name | pIBB477d | Incompatibility group | - |
| Plasmid size | 16577 bp | Coordinate of oriT [Strand] | 7163..7299 [-] |
| Host baterium | Lactococcus cremoris subsp. cremoris IBB477 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |