Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   115507
Name   oriT_EB5|unnamed2 in_silico
Organism   Enterobacter cloacae complex sp. EB5
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP133864 (47000..47105 [+], 106 nt)
oriT length   106 nt
IRs (inverted repeats)      30..35, 41..46  (CCGCCG..CGGCGG)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 106 nt

>oriT_EB5|unnamed2
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   9932 GenBank   WP_004187323
Name   traI_RHN82_RS24335_EB5|unnamed2 insolico UniProt ID   A0A3U8KUL3
Length   659 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 659 a.a.        Molecular weight: 75179.89 Da        Isoelectric Point: 9.9948

>WP_004187323.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(86-334)


  Protein structure


Source ID Structure
AlphaFold DB A0A3U8KUL3


T4CP

ID   11582 GenBank   WP_310399620
Name   t4cp1_RHN82_RS24040_EB5|unnamed2 insolico UniProt ID   _
Length   485 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 485 a.a.        Molecular weight: 55448.09 Da        Isoelectric Point: 8.6416

>WP_310399620.1 conjugal transfer protein TrbC [Enterobacter cloacae complex sp. EB5]
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSRITRRSPAARTARWIR

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 49901..61617

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RHN82_RS24315 (RHN82_24315) 44985..45296 + 312 WP_004187333 hypothetical protein -
RHN82_RS24320 (RHN82_24320) 45429..45965 + 537 WP_004187332 hypothetical protein -
RHN82_RS24325 (RHN82_24325) 46467..46862 - 396 WP_019725163 hypothetical protein -
RHN82_RS24330 (RHN82_24330) 46897..47424 + 528 WP_172740549 plasmid mobilization protein MobA -
RHN82_RS24335 (RHN82_24335) 47411..49390 + 1980 WP_004187323 TraI/MobA(P) family conjugative relaxase -
RHN82_RS24340 (RHN82_24340) 49404..49904 + 501 WP_004187320 DotD/TraH family lipoprotein -
RHN82_RS24345 (RHN82_24345) 49901..50680 + 780 WP_004187315 type IV secretory system conjugative DNA transfer family protein traI
RHN82_RS24350 (RHN82_24350) 50691..51854 + 1164 WP_004187313 plasmid transfer ATPase TraJ virB11
RHN82_RS24355 (RHN82_24355) 51844..52104 + 261 WP_004187310 IcmT/TraK family protein traK
RHN82_RS24360 (RHN82_24360) 52129..55566 + 3438 WP_015586048 LPD7 domain-containing protein -
RHN82_RS24365 (RHN82_24365) 55532..56044 + 513 WP_011091071 hypothetical protein traL
RHN82_RS24370 (RHN82_24370) 56045..56257 - 213 WP_305953721 Hha/YmoA family nucleoid-associated regulatory protein -
RHN82_RS24375 (RHN82_24375) 56229..56639 - 411 WP_004187465 H-NS family nucleoid-associated regulatory protein -
RHN82_RS24380 (RHN82_24380) 56707..57420 + 714 WP_004187467 DotI/IcmL family type IV secretion protein traM
RHN82_RS24385 (RHN82_24385) 57429..58580 + 1152 WP_004187471 DotH/IcmK family type IV secretion protein traN
RHN82_RS24390 (RHN82_24390) 58592..59941 + 1350 WP_004187474 conjugal transfer protein TraO traO
RHN82_RS24395 (RHN82_24395) 59953..60657 + 705 WP_015060002 conjugal transfer protein TraP traP
RHN82_RS24400 (RHN82_24400) 60681..61211 + 531 WP_004187478 conjugal transfer protein TraQ traQ
RHN82_RS24405 (RHN82_24405) 61228..61617 + 390 WP_004187479 DUF6750 family protein traR
RHN82_RS24410 (RHN82_24410) 61663..62157 + 495 WP_004187480 hypothetical protein -

Host bacterium

ID   15940 GenBank   NZ_CP133864
Plasmid name   EB5|unnamed2 Incompatibility group   IncL/M
Plasmid size   63588 bp Coordinate of oriT [Strand]   47000..47105 [+]
Host baterium   Enterobacter cloacae complex sp. EB5

Cargo genes

Drug resistance gene   blaOXA-48
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -