Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   115501
Name   oriT_pEB6_KPC2 in_silico
Organism   Enterobacter hormaechei strain EB6
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP133857 (17558..17812 [+], 255 nt)
oriT length   255 nt
IRs (inverted repeats)      153..158, 160..165  (AAAAGT..ACTTTT)
 122..130, 135..143  (TTAAGGCTT..AAGCCTTAA)
 49..55, 60..66  (GAATTTT..AAAATTC)
Location of nic site      78..79
Conserved sequence flanking the
  nic site    		  TTTGGTTAAA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 255 nt

>oriT_pEB6_KPC2
GGATTTAGGTTTTTTTTTAATCGCTTCACATTTCGTTAGCATGCGCAGGAATTTTTGATAAAATTCTGGTTAGTTTGGTTAAAAAGTGTTACAAGTAAGCGGTGTGGTTGAAGGGATAGATTTAAGGCTTATTCAAGCCTTAAGAAAATACTAAAAGTTACTTTTCACCCTACCGAACACCTAACAAAAAATCCATGTTGAAGATTTGAACAATTGTAATGGCGCAAGGACAATCAGCACATGTCAGAATCTGAT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   9927 GenBank   WP_012477394
Name   mobH_RHB97_RS00080_pEB6_KPC2 insolico UniProt ID   A0A2Z2E7B6
Length   1050 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1050 a.a.        Molecular weight: 118067.47 Da        Isoelectric Point: 4.6347

>WP_012477394.1 MULTISPECIES: MobH family relaxase [Enterobacterales]
MMMNFRALYLCIKRILGIFSSQENDATSVMIEDISSLSPFAQILGDQKYTVPDHPNPEVLKFIEYPTRPT
GIQTFNEQSILSLYREKLHSISMMLAISDSDIRDDAYTFTNLVLKPLVEYVRWIHLLPASENHHHNGIGG
LLSHSLEVAILSLKNAHHSELRPIGYQDEEVVRRKVYLYAAFICGLVHDAGKVYDLDIVSLNLASPIIWT
PSSQSLLDWARENDVVEYEIHWRKRIHNQHNIWSSVFLERILNPVCLAFLDRVNKERVYSKMITALNVYT
DGNDFLSKCVRTADFYSTGTDLNVLRDPIMGLRSNDAAARAISTIKHNFTSININNYNAKPMHIIIVNGE
VYLNENAFLDFVLNDFELHKYNFPQGEAGKTVLVESLVQRGYVEPYDDERVVHYFIPGIYSENEISNIFR
NGIGKLEFYNLLKLRWIGLIFDSYKIPDSVPGLFSVNANKDFIYIDEQKTVTEYRRPVPGRDVITKITDT
VETAVLKVNDLGRSSASIDVDIHSKKNEGSSDDFEKKAESDNEIDNDTQIVKSEGEEAADPVIPDIEESE
DESAKDTESHVLVNQLHELLLSAPLSNDYIVCVDAVPYLNIDTTMALLPGLDEKAFSEEPYFQLTFREGS
LDGMWIVRDIDDLRLVQLGDNCAGFQLTYHEPRRPTTLKSLFNTSMYQALVINDESSVENSAPRPKQTLE
LPPPRVNAVEEHSGDVEYHGTDSASATGPLKTEAVEYEHYQHLFEKEDEEHEIIDYTDFSQLSVSRPEVG
SCATSSSVHNEKLLSEPSELPELNREQNADPQGTNERSMDVSVGQENSEPDTEGNCPPPAEVVYSQTEAA
ATSVMASEEPALPPVLEESNGEHAPTDAKGHHLSPALARLFAPTAPVEKQNPKRNRNKSSDKAEVQKPAS
PVSGHNLNSKVFASTESDQNGEFSLISEGDVTELEFVEIALVLHQILSKMEVAFKRKRKNRFMVSTPNTL
YLTQSCVEKFGSQLEAQDLFNKLPQYLVNSGAVINTKCHAFNMPTLLAASDRAKVDIERIINNLKEAGNL

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(108-304)


  Protein structure


Source ID Structure
AlphaFold DB A0A2Z2E7B6


T4CP

ID   11575 GenBank   WP_001284073
Name   traD_RHB97_RS00075_pEB6_KPC2 insolico UniProt ID   _
Length   694 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 694 a.a.        Molecular weight: 78123.91 Da        Isoelectric Point: 8.0177

>WP_001284073.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Enterobacterales]
MSDSKRTNLHAQENFYRPILEYRSASILLICSVSMLYMGLSSDGLDIAPIVLFTSILLFLLCLYRCKTAA
PFLMAHWRVFKRHFMFVSLDSLRVINKSNFFSNERKYRQLVQDYQNKNKDIPERKSYFCDGFEWGPEHAD
RAYQIANLSSDKREIELPFVFNPIKRHFDAMARKMGGSNAIFAVERREPIFVTEDNWFGHTLITGNVGTG
KTVLQRLLSISMLHLGHVVVVIDPKNDAEWRESLMEEAKTLGLPFYKFHPGQPASSVCIDVCNTYTNVSD
LTSRLLSLVTVPGEVNPFVQYAKALVSNVISGLSYIEKKPSIYLIHKNMKSHMSIVNLTVKVMESCYARY
YGYDVWTEKVKYVANDTLPVRFKRLAEWFTAHFMNYEGSEQIDWLDTVSQLIDYSMSDPEHMAKMTAGIM
PVFDMLIEKPLNELLSPNPNSVSSREIVTSEGMFSTGGVLYISLDGLSNPDTAAAISQLIMSDLTSCAGS
RYNAQDGDMSANSRISIFVDEAHSAINNPMINLLAQGRAAKIALFICTQTISDFIAAASVETANRITGLC
NNYISLRVNDTPTQTLVVENFGKSAISTNMVTYTTGSETSLPHNNFSGSISERKQTTLEESIPKDLLGQV
PMFHIVARLQDGRKVVGQIPIAVAEKQMKPNTTLSEMLFKKAGKVTLRQNLDIKNLNKFLRKLH

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(406-609)

AAA-like domain (AAA_10)

(199-249)

  Protein structure



No available structure.



ID   11576 GenBank   WP_001177101
Name   t4cp2_RHB97_RS00725_pEB6_KPC2 insolico UniProt ID   _
Length   673 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 673 a.a.        Molecular weight: 76680.04 Da        Isoelectric Point: 6.0438

>WP_001177101.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacterales]
MQLRQNLKKSDIISTIIFSILMVGIGGAIGVYGGGYVFLLISKVDTNLLSYDTLIKAWPLWEYKKLHAAI
SLGTIIFAAAIIVPLAAIIIGAFFEFPEPDVHGNAKLITDEEIKESGLLDTDDEFPEILIGKVAKGRYKG
KYLKFAGQQFLGLGAPTRSGKGVGFVIPNLLNYRDSVCVLDIKAENYIFTAGYRKKMGQEVFVFAPDGYR
FSEEEFEEFKFSDYYLNLSPEEQQAFNEKFRNTEHVLSHRWNPLDYISRSKSRRVGEIKDLATILFPDEG
GDNAIWNSMAEAVFESYILYMLDMEETINKINAEIEDNRKNGIDDDRELMKPYPVSMAQLYSLTGVPDMA
EWMNDEILYWKDKGYPLSKGCVEGFNRFIAVDPKTRGNIMQNFNKPLKIFEAESCRLATSASDFDFNDLR
RKRMSVYIVLSPTGMEKYKQLINLFFSQLVRVNTQTLPEHDKTLKYQCLLVLDEFTSMGRVGIIEKSIAF
TAGYNIRYMIIYQNDEQLESDDAYGKSGAFTLRKNLAVEVVYPPKDVDSTAERISKTFGKKTVKIKTNSR
TSNGGKISNGTNEQYVGRDLYMPQEIVELGSQKYVMQKINKGKRTKVAVNEVIIMEQVKPFVAHKIVYFD
EPVFMERRKVATDNVPDVPILQDIEHYEIPRDKRRDIPSIASA

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(109-207)

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(242-641)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 130979..144478

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RHB97_RS00715 (RHB97_00715) 128083..128469 - 387 WP_001076656 hypothetical protein -
RHB97_RS00720 (RHB97_00720) 128555..128920 - 366 WP_000849236 TrbM/KikA/MpfK family conjugal transfer protein -
RHB97_RS00725 (RHB97_00725) 128936..130957 - 2022 WP_001177101 type IV secretory system conjugative DNA transfer family protein -
RHB97_RS00730 (RHB97_00730) 130979..132040 - 1062 WP_001005925 P-type DNA transfer ATPase VirB11 virB11
RHB97_RS00735 (RHB97_00735) 132117..133343 - 1227 WP_000999412 type IV secretion system protein VirB10 virB10
RHB97_RS00740 (RHB97_00740) 133336..134124 - 789 WP_000722128 TrbG/VirB9 family P-type conjugative transfer protein virB9
RHB97_RS00745 (RHB97_00745) 134145..134936 - 792 WP_000476772 virB8 family protein virB8
RHB97_RS00750 (RHB97_00750) 134938..135111 - 174 WP_001128573 hypothetical protein -
RHB97_RS00755 (RHB97_00755) 135161..135841 - 681 WP_001242571 type IV secretion system protein -
RHB97_RS00760 (RHB97_00760) 135825..138350 - 2526 WP_225396201 VirB4 family type IV secretion system protein virb4
RHB97_RS00765 (RHB97_00765) 138313..138711 - 399 WP_000796685 hypothetical protein virB3
RHB97_RS00770 (RHB97_00770) 138772..139089 - 318 WP_001172952 hypothetical protein -
RHB97_RS00775 (RHB97_00775) 139104..139781 - 678 WP_000241898 lytic transglycosylase domain-containing protein virB1
RHB97_RS00780 (RHB97_00780) 139875..140285 - 411 WP_000903582 hypothetical protein -
RHB97_RS00785 (RHB97_00785) 140496..140999 - 504 WP_000288042 LuxR C-terminal-related transcriptional regulator -
RHB97_RS00790 (RHB97_00790) 141000..142064 - 1065 WP_014710721 replication initiation protein -
RHB97_RS00795 (RHB97_00795) 143158..143406 + 249 WP_000570802 hypothetical protein -
RHB97_RS00800 (RHB97_00800) 143426..144478 + 1053 WP_000394663 type IV secretion system protein virB6
RHB97_RS00805 (RHB97_00805) 144591..145172 + 582 WP_000785684 hypothetical protein -
RHB97_RS00810 (RHB97_00810) 145270..145527 + 258 WP_001045458 hypothetical protein -
RHB97_RS00815 (RHB97_00815) 145754..145909 + 156 WP_001015890 hypothetical protein -
RHB97_RS00820 (RHB97_00820) 146029..146334 + 306 WP_225396203 hypothetical protein -
RHB97_RS00825 (RHB97_00825) 146574..147278 + 705 WP_001067855 IS6-like element IS26 family transposase -

Region 2: 254930..264998

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RHB97_RS01380 (RHB97_01380) 252220..252381 - 162 WP_001371949 hypothetical protein -
RHB97_RS01385 (RHB97_01385) 254930..257611 - 2682 WP_000387412 TraC family protein virb4
RHB97_RS01390 (RHB97_01390) 257620..258570 - 951 WP_001022588 IncHI-type conjugal transfer lipoprotein TrhV traV
RHB97_RS01395 (RHB97_01395) 258580..259332 - 753 WP_182911723 protein-disulfide isomerase HtdT -
RHB97_RS01400 (RHB97_01400) 259451..259933 - 483 WP_000377632 hypothetical protein -
RHB97_RS01405 (RHB97_01405) 259941..261296 - 1356 WP_000351841 IncHI-type conjugal transfer protein TrhB traB
RHB97_RS01410 (RHB97_01410) 261286..261747 - 462 WP_000521242 plasmid transfer protein HtdO -
RHB97_RS01415 (RHB97_01415) 261749..263020 - 1272 WP_000592092 type-F conjugative transfer system secretin TraK traK
RHB97_RS01420 (RHB97_01420) 263020..263532 - 513 WP_249540343 type IV conjugative transfer system protein TraE traE
RHB97_RS01425 (RHB97_01425) 263603..264307 - 705 WP_001067855 IS6-like element IS26 family transposase -
RHB97_RS01430 (RHB97_01430) 264319..264636 - 318 WP_309883531 hypothetical protein traE
RHB97_RS01435 (RHB97_01435) 264648..264998 - 351 WP_001447770 type IV conjugative transfer system protein TraL traL
RHB97_RS01440 (RHB97_01440) 265016..265369 - 354 WP_000423602 hypothetical protein -
RHB97_RS01445 (RHB97_01445) 266518..267393 - 876 WP_000594611 RepB family plasmid replication initiator protein -
RHB97_RS01450 (RHB97_01450) 267870..268922 + 1053 WP_001065778 hypothetical protein -
RHB97_RS01455 (RHB97_01455) 268942..269142 + 201 WP_000594930 hypothetical protein -
RHB97_RS01460 (RHB97_01460) 269139..269855 + 717 WP_000838219 zinc finger-like domain-containing protein -

Host bacterium

ID   15934 GenBank   NZ_CP133857
Plasmid name   pEB6_KPC2 Incompatibility group   -
Plasmid size   299734 bp Coordinate of oriT [Strand]   17558..17812 [+]
Host baterium   Enterobacter hormaechei strain EB6

Cargo genes

Drug resistance gene   mcr-9, blaCTX-M-9, sul1, qacE, aadA2, dfrA16, blaKPC-2, tet(A), ant(2'')-Ia
Virulence gene   -
Metal resistance gene   arsH, arsR, arsB, arsC, rcnR/yohL, ncrC, pcoE, pcoS, merR, merT, merP, merC, merA, merD, merE, terE, terD, terC, terB, terA, terZ, terW
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -