Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 115474 |
| Name | oriT_pHL17078_1  |
| Organism | Staphylococcus aureus strain HL17078 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP080557 (24821..24856 [-], 36 nt) |
| oriT length | 36 nt |
| IRs (inverted repeats) | 4..10, 14..20 (GCGAACG..CGTTCGC) |
| Location of nic site | 29..30 |
| Conserved sequence flanking the nic site |
GTGCGCCCTT |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 36 nt
>oriT_pHL17078_1
CACGCGAACGGAACGTTCGCATAAGTGCGCCCTTAC
CACGCGAACGGAACGTTCGCATAAGTGCGCCCTTAC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 9910 | GenBank | WP_001252101 |
| Name | MobA_MobL_JMO10_RS14920_pHL17078_1 |
UniProt ID | A0A141HMI9 |
| Length | 665 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 665 a.a. Molecular weight: 78908.86 Da Isoelectric Point: 5.3910
>WP_001252101.1 MULTISPECIES: MobA/MobL family protein [Bacilli]
MAMYHFQNKFVSKANGQSATAKSAYNSASRIKDFKENEFKDYSNKQCDYSEILLPNNADDKFKDREYLWN
KVHDVENRKNSQVAREIIIGLPNEFDPNSNIELAKEFAESLSNEGMIVDLNIHKINEENPHAHLLCTLRG
LDKNNEFEPKRKGNDYIRDWNTKEKHNEWRKRWENVQNKHLEKNGFSVRVSADSYKNQNIDLEPTKKEGW
KARKFEDETGKKSSISKHNESIKKRNQQKIDKMFNEVDDMKSHKLNAFSYMNKSDSTTLKNMAKDLKIYV
TPINMYKENERLYDLKQKTSLITDDEDRLNKIEDIEDRQKKLESINEVFEKQAGIFFDKNYPDQSLNYSD
DEKIFITRTILNDRDVLPANNELEDIVKEKRIKEAQISLNTVLGNRDISLESIEKESNFFADKLSNILEK
NNLSFDDVLENKHEGMEDSLKIDYYTNKLEVFRNAENILEDYYDVQIKELFTDDEDYKAFNEVTDIKEKQ
QLIDFKTYHGTENTIEMLETGNFIPKYSDEDRKYITEQVKLLQEKEFKPNKNQHDKFVFGAIQKKLLSEY
DFDYSDNNDLKHLYQESNEVGDEISKDNIEEFYEGNDILVDKETYNNYNRKQQAYGLINAGLDSMIFNFN
EIFRERMPKYINHQYKGKNHSKQRHELKNKRGMHL
MAMYHFQNKFVSKANGQSATAKSAYNSASRIKDFKENEFKDYSNKQCDYSEILLPNNADDKFKDREYLWN
KVHDVENRKNSQVAREIIIGLPNEFDPNSNIELAKEFAESLSNEGMIVDLNIHKINEENPHAHLLCTLRG
LDKNNEFEPKRKGNDYIRDWNTKEKHNEWRKRWENVQNKHLEKNGFSVRVSADSYKNQNIDLEPTKKEGW
KARKFEDETGKKSSISKHNESIKKRNQQKIDKMFNEVDDMKSHKLNAFSYMNKSDSTTLKNMAKDLKIYV
TPINMYKENERLYDLKQKTSLITDDEDRLNKIEDIEDRQKKLESINEVFEKQAGIFFDKNYPDQSLNYSD
DEKIFITRTILNDRDVLPANNELEDIVKEKRIKEAQISLNTVLGNRDISLESIEKESNFFADKLSNILEK
NNLSFDDVLENKHEGMEDSLKIDYYTNKLEVFRNAENILEDYYDVQIKELFTDDEDYKAFNEVTDIKEKQ
QLIDFKTYHGTENTIEMLETGNFIPKYSDEDRKYITEQVKLLQEKEFKPNKNQHDKFVFGAIQKKLLSEY
DFDYSDNNDLKHLYQESNEVGDEISKDNIEEFYEGNDILVDKETYNNYNRKQQAYGLINAGLDSMIFNFN
EIFRERMPKYINHQYKGKNHSKQRHELKNKRGMHL
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A141HMI9 |
T4CP
| ID | 11553 | GenBank | WP_000209436 |
| Name | t4cp2_JMO10_RS14810_pHL17078_1 |
UniProt ID | _ |
| Length | 546 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 546 a.a. Molecular weight: 62672.60 Da Isoelectric Point: 7.6569
>WP_000209436.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Bacilli]
MTTLLGELESKATSINKKNLVIQDFDTKCPNRNIFVVGGPGSYKSAGYVIPNVIVKNQQSIVVTDPSGEV
YEKTSNIKRMQGFDVRVVNFKNFLASDRQNFFDYIDKDSDCSIVAELIIKSAGDSKINKDVWYKASVGLL
NSLLLYAKYEFEPEKRTIGNIIKFIQNNKPNQDEEGSVELDKRFNELSKDHPARESYEFGFAVSEGRTRA
SIILTFVADLRNYIDNEIRSYTSDSDFDLRDVGLRKTIIYVMLPVLGNTVQSLSSLFFSQMFQQLYRLGD
ENGARLPVPVDFLLDEWPNIGAIPDYEETLATCRKYGISITTIVQSISQAVDKYNKDKANAIIGNHAVIL
CLGNVNNDTAKYIKEELGNATVEFETSSEGSSSGKDSRSKSSNKNVSYTGRALLNEDEISNMQQDKAILI
TKNRNPKIIKKLAYFKMFPNLTETYIAPQNEYKRKKNQSMIDKHNRLREEWDKKQEKNKQQKLEEYKEEQ
RQKELEKEQQPQEEQQNEKVKESKDKNDQEDKKDEQQKINDSKKAFYEKLKQKQAK
MTTLLGELESKATSINKKNLVIQDFDTKCPNRNIFVVGGPGSYKSAGYVIPNVIVKNQQSIVVTDPSGEV
YEKTSNIKRMQGFDVRVVNFKNFLASDRQNFFDYIDKDSDCSIVAELIIKSAGDSKINKDVWYKASVGLL
NSLLLYAKYEFEPEKRTIGNIIKFIQNNKPNQDEEGSVELDKRFNELSKDHPARESYEFGFAVSEGRTRA
SIILTFVADLRNYIDNEIRSYTSDSDFDLRDVGLRKTIIYVMLPVLGNTVQSLSSLFFSQMFQQLYRLGD
ENGARLPVPVDFLLDEWPNIGAIPDYEETLATCRKYGISITTIVQSISQAVDKYNKDKANAIIGNHAVIL
CLGNVNNDTAKYIKEELGNATVEFETSSEGSSSGKDSRSKSSNKNVSYTGRALLNEDEISNMQQDKAILI
TKNRNPKIIKKLAYFKMFPNLTETYIAPQNEYKRKKNQSMIDKHNRLREEWDKKQEKNKQQKLEEYKEEQ
RQKELEKEQQPQEEQQNEKVKESKDKNDQEDKKDEQQKINDSKKAFYEKLKQKQAK
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 4909..16130
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| JMO10_RS14765 (JMO10_002954) | 1..675 | + | 675 | WP_001105984 | IS6-like element IS257 family transposase | - |
| JMO10_RS14770 (JMO10_002955) | 736..1110 | + | 375 | Protein_1 | IS256 family transposase | - |
| JMO10_RS14775 (JMO10_002956) | 1167..1559 | + | 393 | WP_000393259 | GNAT family N-acetyltransferase | - |
| JMO10_RS14780 (JMO10_002957) | 1560..2999 | + | 1440 | WP_001028144 | aminoglycoside O-phosphotransferase APH(2'')-Ia | - |
| JMO10_RS14785 (JMO10_002958) | 3129..3503 | - | 375 | Protein_4 | IS256 family transposase | - |
| JMO10_RS14790 (JMO10_002959) | 3564..4238 | - | 675 | WP_001105984 | IS6-like element IS257 family transposase | - |
| JMO10_RS14795 (JMO10_002960) | 4276..4443 | - | 168 | WP_000869301 | hypothetical protein | - |
| JMO10_RS14800 (JMO10_002961) | 4500..4892 | - | 393 | WP_000608970 | single-stranded DNA-binding protein | - |
| JMO10_RS14805 (JMO10_002962) | 4909..5826 | - | 918 | WP_000383804 | conjugal transfer protein TrbL family protein | trsL |
| JMO10_RS14810 (JMO10_002963) | 5904..7544 | - | 1641 | WP_000209436 | VirD4-like conjugal transfer protein, CD1115 family | - |
| JMO10_RS14815 (JMO10_002964) | 7541..8005 | - | 465 | WP_000715273 | hypothetical protein | trsJ |
| JMO10_RS14820 (JMO10_002965) | 8021..10123 | - | 2103 | WP_001094109 | DNA topoisomerase III | - |
| JMO10_RS14825 (JMO10_002966) | 10139..10624 | - | 486 | WP_000735569 | TrsH/TraH family protein | - |
| JMO10_RS14830 (JMO10_002967) | 10634..11710 | - | 1077 | WP_000269385 | CHAP domain-containing protein | trsG |
| JMO10_RS14835 (JMO10_002968) | 11728..13008 | - | 1281 | WP_000702361 | membrane protein | trsF |
| JMO10_RS14840 (JMO10_002969) | 13020..15032 | - | 2013 | WP_000874919 | VirB4 family type IV secretion system protein | virb4 |
| JMO10_RS14845 (JMO10_002970) | 15053..15736 | - | 684 | WP_000979860 | TrsD/TraD family conjugative transfer protein | trsD |
| JMO10_RS14850 (JMO10_002971) | 15723..16130 | - | 408 | WP_000110157 | hypothetical protein | trsC |
| JMO10_RS14855 (JMO10_002972) | 16190..16507 | - | 318 | WP_000591622 | CagC family type IV secretion system protein | - |
| JMO10_RS14860 (JMO10_002973) | 16524..17498 | - | 975 | WP_000368849 | conjugative transfer protein TrsA | - |
| JMO10_RS14865 (JMO10_002974) | 17670..17858 | + | 189 | WP_001063224 | hypothetical protein | - |
| JMO10_RS14870 (JMO10_002975) | 17967..18641 | - | 675 | WP_001106019 | IS6-like element IS257 family transposase | - |
| JMO10_RS14875 (JMO10_002976) | 18685..18831 | + | 147 | WP_001817822 | hypothetical protein | - |
| JMO10_RS14880 (JMO10_002977) | 18991..19761 | + | 771 | WP_001795128 | aminoglycoside O-nucleotidyltransferase ANT(4')-Ia | - |
| JMO10_RS14885 (JMO10_002978) | 20090..20764 | - | 675 | WP_001106022 | IS6-like element IS257 family transposase | - |
Host bacterium
| ID | 15907 | GenBank | NZ_CP080557 |
| Plasmid name | pHL17078_1 | Incompatibility group | - |
| Plasmid size | 42253 bp | Coordinate of oriT [Strand] | 24821..24856 [-] |
| Host baterium | Staphylococcus aureus strain HL17078 |
Cargo genes
| Drug resistance gene | aac(6')-aph(2''), aadD, mupA |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIA21 |