Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 115462 |
| Name | oriT_p16055-KPC  |
| Organism | Serratia marcescens strain 160316055 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_MN823985 (105163..105211 [+], 49 nt) |
| oriT length | 49 nt |
| IRs (inverted repeats) | 6..13, 16..23 (GCAAAATT..AATTTTGC) |
| Location of nic site | 32..33 |
| Conserved sequence flanking the nic site |
GGTGTGGTGA |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 49 nt
>oriT_p16055-KPC
AATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTGATTTTGTGGTGAG
AATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTGATTTTGTGGTGAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 9903 | GenBank | WP_064291677 |
| Name | traI_H5G91_RS00465_p16055-KPC |
UniProt ID | A0A6I4Y321 |
| Length | 1753 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 1753 a.a. Molecular weight: 190676.77 Da Isoelectric Point: 6.3386
>WP_064291677.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Enterobacterales]
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGIDGKAVDKALFTELLKGKLPDGSDLTRI
QDGANKHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVTEAVRQLETLAATRVMTDGKSETVLTGNL
IVAKFNHDTNRNQEPQIHTHAVVINATQNGDKWQSLGTDKIGKTGFIENVYANQIAFGKLYREAFKPLVE
KLGYETEVVGKHGMWEMKGVPVEPFSTRSQEVREAAGPDASLKSRDVAALDTRKSKDAIDPAEKMVEWMN
TLKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLE
AKDGVFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVP
FSDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVT
GKSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTY
RWQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKD
TTITALTPVWLDSKSRGVRDYYREGMVMERWDPETRTHDRFVIDRVTGSSNMLTLKDREGVRLDLKVSAV
DSQWTLFRADTLPVAEGERLAVLGKIPDTRLKGGESITVMKVEEGQLTVQRPGQKTTQTLAVGAGVFDGI
KIGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSE
QLKTRSGETDLDAAIAQQKAGLRTPAEQAIHLAIPLLESEKLTFSRPQLLATALETGGGKVSMADIDTTI
QAQIRSGQLLNVPVAHGYGNDLLISRQTWDAEKSILTHVLEGKDAVAPLMDRVPASLMTDLTAGQRAATR
MILESTDRFTVVQGYAGVGKTTQLRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLH
DTQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIVAGGGRAVSSGDNDQLQPIAPGQPFRLTQQR
SAADVAIMKEIVRQMPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGAWAPGSSVVEFTPKQEKAIE
KALSEGKTLPEGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITL
PVLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKANQLITLTDSEGKERYISPREASAEGVTL
YRQEKITVSQGDRMRFSKSDLERGYVANSIWEVQSVSGDSVTLSDGKLTRTLTPKADQAQQHIDLAYAIT
AHGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDNREGWIKAIKHSPEKATAHDILE
PRNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGI
WLSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDR
PWNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEP
ADRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEEAIQQVASERLQRERLQAVERDMVRDLNREKTL
GGD
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGIDGKAVDKALFTELLKGKLPDGSDLTRI
QDGANKHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVTEAVRQLETLAATRVMTDGKSETVLTGNL
IVAKFNHDTNRNQEPQIHTHAVVINATQNGDKWQSLGTDKIGKTGFIENVYANQIAFGKLYREAFKPLVE
KLGYETEVVGKHGMWEMKGVPVEPFSTRSQEVREAAGPDASLKSRDVAALDTRKSKDAIDPAEKMVEWMN
TLKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLE
AKDGVFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVP
FSDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVT
GKSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTY
RWQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKD
TTITALTPVWLDSKSRGVRDYYREGMVMERWDPETRTHDRFVIDRVTGSSNMLTLKDREGVRLDLKVSAV
DSQWTLFRADTLPVAEGERLAVLGKIPDTRLKGGESITVMKVEEGQLTVQRPGQKTTQTLAVGAGVFDGI
KIGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSE
QLKTRSGETDLDAAIAQQKAGLRTPAEQAIHLAIPLLESEKLTFSRPQLLATALETGGGKVSMADIDTTI
QAQIRSGQLLNVPVAHGYGNDLLISRQTWDAEKSILTHVLEGKDAVAPLMDRVPASLMTDLTAGQRAATR
MILESTDRFTVVQGYAGVGKTTQLRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLH
DTQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIVAGGGRAVSSGDNDQLQPIAPGQPFRLTQQR
SAADVAIMKEIVRQMPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGAWAPGSSVVEFTPKQEKAIE
KALSEGKTLPEGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITL
PVLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKANQLITLTDSEGKERYISPREASAEGVTL
YRQEKITVSQGDRMRFSKSDLERGYVANSIWEVQSVSGDSVTLSDGKLTRTLTPKADQAQQHIDLAYAIT
AHGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDNREGWIKAIKHSPEKATAHDILE
PRNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGI
WLSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDR
PWNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEP
ADRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEEAIQQVASERLQRERLQAVERDMVRDLNREKTL
GGD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 11543 | GenBank | WP_072101984 |
| Name | traD_H5G91_RS00470_p16055-KPC |
UniProt ID | _ |
| Length | 770 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 770 a.a. Molecular weight: 85967.95 Da Isoelectric Point: 5.0577
>WP_072101984.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacterales]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDDDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSREIS
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDTPASGPADTDSHASEQPEPVSPPA
PAVMTVTPAPVKSPPTTKRPAAEPSVRATEPPVLRGTTVPLIKPKAAAAATAASTASSAGAPAAAAGGTE
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLTDEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDDDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSREIS
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDTPASGPADTDSHASEQPEPVSPPA
PAVMTVTPAPVKSPPTTKRPAAEPSVRATEPPVLRGTTVPLIKPKAAAAATAASTASSAGAPAAAAGGTE
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLTDEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 76519..105769
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| H5G91_RS00470 | 76519..78831 | - | 2313 | WP_072101984 | type IV conjugative transfer system coupling protein TraD | virb4 |
| H5G91_RS00475 | 78960..79649 | - | 690 | WP_072101983 | hypothetical protein | - |
| H5G91_RS00480 | 79842..80573 | - | 732 | WP_004152629 | conjugal transfer complement resistance protein TraT | - |
| H5G91_RS00485 | 80759..81292 | - | 534 | WP_014343486 | conjugal transfer protein TraS | - |
| H5G91_RS00490 | 81295..84144 | - | 2850 | WP_013609534 | conjugal transfer mating-pair stabilization protein TraG | traG |
| H5G91_RS00495 | 84144..85523 | - | 1380 | WP_014343487 | conjugal transfer pilus assembly protein TraH | traH |
| H5G91_RS00500 | 85501..85944 | - | 444 | WP_004194305 | F-type conjugal transfer protein TrbF | - |
| H5G91_RS00505 | 85990..86547 | - | 558 | WP_013214031 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
| H5G91_RS00510 | 86519..86758 | - | 240 | WP_004144400 | type-F conjugative transfer system pilin chaperone TraQ | - |
| H5G91_RS00515 | 86769..87521 | - | 753 | WP_004152677 | type-F conjugative transfer system pilin assembly protein TraF | traF |
| H5G91_RS00520 | 87542..87868 | - | 327 | WP_004152676 | hypothetical protein | - |
| H5G91_RS00525 | 87881..88129 | - | 249 | WP_004152675 | hypothetical protein | - |
| H5G91_RS00530 | 88107..88361 | - | 255 | WP_004152674 | conjugal transfer protein TrbE | - |
| H5G91_RS00535 | 88393..90348 | - | 1956 | WP_030003482 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
| H5G91_RS00540 | 90407..91045 | - | 639 | WP_069985712 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
| H5G91_RS00545 | 91058..92047 | - | 990 | WP_029497420 | conjugal transfer pilus assembly protein TraU | traU |
| H5G91_RS00550 | 92044..92445 | - | 402 | WP_004194979 | hypothetical protein | - |
| H5G91_RS00555 | 92480..93115 | - | 636 | WP_029497419 | type-F conjugative transfer system protein TraW | traW |
| H5G91_RS00560 | 93115..93504 | - | 390 | WP_004167468 | type-F conjugative transfer system protein TrbI | - |
| H5G91_RS00565 | 93504..96143 | - | 2640 | WP_013214024 | type IV secretion system protein TraC | virb4 |
| H5G91_RS00570 | 96215..96613 | - | 399 | WP_023179972 | hypothetical protein | - |
| H5G91_RS00575 | 96992..97396 | - | 405 | WP_004197817 | hypothetical protein | - |
| H5G91_RS00580 | 97463..97774 | - | 312 | WP_029497417 | hypothetical protein | - |
| H5G91_RS00585 | 97775..97993 | - | 219 | WP_004195468 | hypothetical protein | - |
| H5G91_RS00590 | 98316..98900 | - | 585 | WP_032440547 | type IV conjugative transfer system lipoprotein TraV | traV |
| H5G91_RS00595 | 98973..100397 | - | 1425 | WP_004194260 | F-type conjugal transfer pilus assembly protein TraB | traB |
| H5G91_RS00600 | 100397..101137 | - | 741 | WP_013023821 | type-F conjugative transfer system secretin TraK | traK |
| H5G91_RS00605 | 101124..101690 | - | 567 | WP_004144423 | type IV conjugative transfer system protein TraE | traE |
| H5G91_RS00610 | 101710..102015 | - | 306 | WP_004144424 | type IV conjugative transfer system protein TraL | traL |
| H5G91_RS00615 | 102029..102397 | - | 369 | WP_004194426 | type IV conjugative transfer system pilin TraA | - |
| H5G91_RS00625 | 102752..103108 | - | 357 | WP_258557319 | hypothetical protein | - |
| H5G91_RS00630 | 103044..103741 | + | 698 | WP_223174630 | IS1 family transposase | - |
| H5G91_RS00635 | 103676..104230 | - | 555 | WP_258557320 | hypothetical protein | - |
| H5G91_RS00640 | 104460..104852 | - | 393 | WP_004194114 | conjugal transfer relaxosome DNA-binding protein TraM | - |
| H5G91_RS00645 | 105284..105769 | + | 486 | WP_032440470 | transglycosylase SLT domain-containing protein | virB1 |
| H5G91_RS00650 | 105802..106131 | - | 330 | WP_071994324 | DUF5983 family protein | - |
| H5G91_RS00655 | 106164..106985 | - | 822 | WP_023287138 | DUF932 domain-containing protein | - |
| H5G91_RS00660 | 107815..108228 | - | 414 | WP_069985713 | type II toxin-antitoxin system HigA family antitoxin | - |
| H5G91_RS00665 | 108229..108507 | - | 279 | WP_004152721 | helix-turn-helix transcriptional regulator | - |
Host bacterium
| ID | 15895 | GenBank | NZ_MN823985 |
| Plasmid name | p16055-KPC | Incompatibility group | IncR |
| Plasmid size | 108662 bp | Coordinate of oriT [Strand] | 105163..105211 [+] |
| Host baterium | Serratia marcescens strain 160316055 |
Cargo genes
| Drug resistance gene | blaKPC-2 |
| Virulence gene | - |
| Metal resistance gene | merR, merT, merP, merA, merD, merE |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIE9 |