Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 115460 |
| Name | oriT_pG426-FII  |
| Organism | Leclercia adecarboxylata strain G426 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_MN842294 (30721..30776 [-], 56 nt) |
| oriT length | 56 nt |
| IRs (inverted repeats) | 16..22, 26..32 (GCAAGAC..GTCTTGC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 56 nt
>oriT_pG426-FII
TTAAAGATAAAATCTGCAAGACAACGTCTTGCGTGGGGTGTGATGTTTTAGATGGT
TTAAAGATAAAATCTGCAAGACAACGTCTTGCGTGGGGTGTGATGTTTTAGATGGT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 9901 | GenBank | WP_181726267 |
| Name | traI_H5H33_RS00335_pG426-FII |
UniProt ID | _ |
| Length | 1810 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 1810 a.a. Molecular weight: 196492.07 Da Isoelectric Point: 6.2532
>WP_181726267.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Enterobacteriaceae]
MMSVAPVASAGSAAGYYSNSDNYYFLGNLQSLWLGEGAKELGLEGNVRGGDLTAVLEGRLPDGSRLGREI
NGNHVHRPGHDLTFSAPKSVSILGLIGGDKEMVEAHNHAVQVAAGYVEKLISARATKDGITSIVHTGKMV
AAAFTHDTSRNLDPQLHTHLLIANMTEHDGKWKALATDYIHNAGFIETVMKMQVTLGKIYRGALRQRVEA
LGHEVEEVGKHGMWEIKGVPEAVREEFSSRGREIEGAIGAEATLRSRDVAAKDTRQAKVDPSRLRLMERW
LGQMKEKGFDLKAYQESVVPRESGLRDAPPQPDRATPVPASRAEPGAVTRSAPEEKTGKSPESPEVPAER
DDTARQPGSHPADTLRQPSLTDPEKTLVPSAASPARNLHADIPQPELAESVRLAISQLSDARTRFTFGEL
MLTTAELSEQLPEIKDIRVAIDAALKDGMIVPLDSEKGVFTSRIHLLDELSIQALSQEHLKSTKVVSFTR
PAQYAPPALAVAETDPLVLMNAPSGVAGIRDLTVQLTDISAANGRDVQVLASSAERAISLARSDTLRDRL
LSRQHILSGDFHLKPQSTLIIEGAERLGLKETLVLLGEARAQDAQLVFLDSAGRQANGNAMSVLESAGVA
RSRRTEPAPGLEAEVISIANKRDRYEALANRFAELSGGSENVTAVVVGKREQAQLTGLVRDALQNAGQLG
RDGAEIEARQPVWLDSKTRRMPGSYRPGMVLEDRRDAKARKSYVIDRVHDDTRVLSLIDGDGVLTRMKIG
EVSADWRLFSSETLNVATGEKLLAVAGDREHSLKAKDRLEVTAITDKGIEVKRGDDTLTLPKDQPLYVTH
AYVSAPGGRDNDTGVVLAALNTRDISTQTMNSLAQSGHRAEVFTAEVQDRAEARLQRMKTNASPVQLVRS
LSGREDVSEAVGSLHDAVRTDAGLAVWRAVNDQRNVVFSELKLAEAASEYHPDLVAVGNEIAGMVKGGEL
LRVSVRGEPQLIARSTWEMEKAILRTVEAGKNTQQPLLDNVPQAVLNGLTSGQKQSTTLVLGSTDQFTGI
QGYAGVGKTTQIKAVVAALDTLPADARPQLTGLAPTHQAVKEMSDVGVRAQTIKSFIVEHDQAKAAGEKP
DYQGQVFLIDESSMAGNQDTAALFQAIAAGGGRAVSMGDTDQFEAVDSGAPFKLMQERSPMDVAIMKEIV
RQKDVQLRGAVHDIIDNRIGAALKRIEAQPADRIAREAGASLPQSAFEETGSPVNDIVSDWTGRTPDARA
RTLIITQLNADRNAVNAGIHDQLVKRGELGREAVTVPVLDKISHTRHEFNKTAAWDANLVVKRGDRYQDV
LAVDRNGNTVTVRDEEGKIGLYSPKELITGDVQLFRRSQIEVRSGDQLRFTLTDREHGQTGNQRFTVDAV
SQTGDIRLRDERGTVTINPGNVRAQQHIDYAWAVTGYGAQGASTDYVISLEGTEGGRKMLASKRAFYISA
SRAKEHVQIYTDGKADWTKAVAAPERDIKTAHDALAPETQRQQAKAIWAMGQPVTKTAIGRAWVRHQEMG
EASLTAKVIPATRRFPEPALALPVYDNNGKSAGLALVSLVSSPEGRLTQGDVRMVMTERARGAVLQRSQS
GNTVVVNDLAAALDAVRTHPKDGVVWQTGQEPPSANLMKVSGGVREDEGLRLNLSTLTAQDELQRQSELK
LAEQAREQDAVRQQQDDTRAIVLPAEEAVKLRPEELNPEKPAQLIPDNNVLASVRERDQADGRELQLADS
VRKDIAVPDNSAGEERRASRVVTDLARAEQDMVRQAADSGRGRIPEIEEQTLTRTIQKER
MMSVAPVASAGSAAGYYSNSDNYYFLGNLQSLWLGEGAKELGLEGNVRGGDLTAVLEGRLPDGSRLGREI
NGNHVHRPGHDLTFSAPKSVSILGLIGGDKEMVEAHNHAVQVAAGYVEKLISARATKDGITSIVHTGKMV
AAAFTHDTSRNLDPQLHTHLLIANMTEHDGKWKALATDYIHNAGFIETVMKMQVTLGKIYRGALRQRVEA
LGHEVEEVGKHGMWEIKGVPEAVREEFSSRGREIEGAIGAEATLRSRDVAAKDTRQAKVDPSRLRLMERW
LGQMKEKGFDLKAYQESVVPRESGLRDAPPQPDRATPVPASRAEPGAVTRSAPEEKTGKSPESPEVPAER
DDTARQPGSHPADTLRQPSLTDPEKTLVPSAASPARNLHADIPQPELAESVRLAISQLSDARTRFTFGEL
MLTTAELSEQLPEIKDIRVAIDAALKDGMIVPLDSEKGVFTSRIHLLDELSIQALSQEHLKSTKVVSFTR
PAQYAPPALAVAETDPLVLMNAPSGVAGIRDLTVQLTDISAANGRDVQVLASSAERAISLARSDTLRDRL
LSRQHILSGDFHLKPQSTLIIEGAERLGLKETLVLLGEARAQDAQLVFLDSAGRQANGNAMSVLESAGVA
RSRRTEPAPGLEAEVISIANKRDRYEALANRFAELSGGSENVTAVVVGKREQAQLTGLVRDALQNAGQLG
RDGAEIEARQPVWLDSKTRRMPGSYRPGMVLEDRRDAKARKSYVIDRVHDDTRVLSLIDGDGVLTRMKIG
EVSADWRLFSSETLNVATGEKLLAVAGDREHSLKAKDRLEVTAITDKGIEVKRGDDTLTLPKDQPLYVTH
AYVSAPGGRDNDTGVVLAALNTRDISTQTMNSLAQSGHRAEVFTAEVQDRAEARLQRMKTNASPVQLVRS
LSGREDVSEAVGSLHDAVRTDAGLAVWRAVNDQRNVVFSELKLAEAASEYHPDLVAVGNEIAGMVKGGEL
LRVSVRGEPQLIARSTWEMEKAILRTVEAGKNTQQPLLDNVPQAVLNGLTSGQKQSTTLVLGSTDQFTGI
QGYAGVGKTTQIKAVVAALDTLPADARPQLTGLAPTHQAVKEMSDVGVRAQTIKSFIVEHDQAKAAGEKP
DYQGQVFLIDESSMAGNQDTAALFQAIAAGGGRAVSMGDTDQFEAVDSGAPFKLMQERSPMDVAIMKEIV
RQKDVQLRGAVHDIIDNRIGAALKRIEAQPADRIAREAGASLPQSAFEETGSPVNDIVSDWTGRTPDARA
RTLIITQLNADRNAVNAGIHDQLVKRGELGREAVTVPVLDKISHTRHEFNKTAAWDANLVVKRGDRYQDV
LAVDRNGNTVTVRDEEGKIGLYSPKELITGDVQLFRRSQIEVRSGDQLRFTLTDREHGQTGNQRFTVDAV
SQTGDIRLRDERGTVTINPGNVRAQQHIDYAWAVTGYGAQGASTDYVISLEGTEGGRKMLASKRAFYISA
SRAKEHVQIYTDGKADWTKAVAAPERDIKTAHDALAPETQRQQAKAIWAMGQPVTKTAIGRAWVRHQEMG
EASLTAKVIPATRRFPEPALALPVYDNNGKSAGLALVSLVSSPEGRLTQGDVRMVMTERARGAVLQRSQS
GNTVVVNDLAAALDAVRTHPKDGVVWQTGQEPPSANLMKVSGGVREDEGLRLNLSTLTAQDELQRQSELK
LAEQAREQDAVRQQQDDTRAIVLPAEEAVKLRPEELNPEKPAQLIPDNNVLASVRERDQADGRELQLADS
VRKDIAVPDNSAGEERRASRVVTDLARAEQDMVRQAADSGRGRIPEIEEQTLTRTIQKER
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 11541 | GenBank | WP_181726320 |
| Name | traD_H5H33_RS00330_pG426-FII |
UniProt ID | _ |
| Length | 871 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 871 a.a. Molecular weight: 97223.18 Da Isoelectric Point: 5.1662
>WP_181726320.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacteriaceae]
MSFAGKNLTQGGQMTAYRWRMFLQVNNWIGFWIFILFAALTSAIFFWRIPQETLNNGSLWWFAWMNSSFI
DLMPATGTPTIYDVHYWYAPTATAMVLNMTLPQIYSDPYMQVMGDQCLRELEWAAGASGIFSMVVFAAVT
WFIADIGRKESEDEYISGMQLTDRPAEVNTLLRKNGELSDLRVGDLHMVRMAEVLNFLMHGTIGVGKSTL
IRWLLDYIRKRGDRAIIYDSGCTFTETHYNPSTDFILNAHDERCANWHMWGECVDAVDYDNLAASLIPVE
GESDPFWVSSSRTIFADLAIRMSADPQRSIEKFLKTLLSLSMKSLRDYLANTPSANLVEEKIEKTAISIR
SVVTNYAKALRYLQGLDDGTKPPFTIREWMTQDRYDSSWLFISTQARHRKSVRPLISLWVSLATLMLQSM
GENSERRVWFIIDEAPSLQRIPELAETLAEARKFGGCFVLGMQNMAQLVHVYGRELAKSIFDLMNTRMYG
RSPSAEMAKVVEEELGNQRRRKIREQNSYGLDQVRDGVSLGKDEVNDPVVDYEQIMRLPNLSFYVRLPGE
YPVVRLKLKYRALKKRQAGLMERNIRDALSPELEKVIQENERAATAAGLTFPTGDEVLEKGTEITPAAGS
AAPVSSSGAPAGLTPREEKPVTEPAAGKKTARARPVIRNLRPVTVPVISEVQTTIAESEPDNHREVTAPV
TSSGESNVISLLHGRNSSAGTAPEAPVTDESAPSMSSEPEVAETPAEQATLPAAVQRLRNLTTAPEQPAT
SVLSILQKARQRPAPSESNSGTGDNNGGESMNLEMKVTALPDGGLSVTTAGKPVTPANPDEAHRTNRRLA
QEEENILLHRDAGDPGYDENDQHYYDREPEL
MSFAGKNLTQGGQMTAYRWRMFLQVNNWIGFWIFILFAALTSAIFFWRIPQETLNNGSLWWFAWMNSSFI
DLMPATGTPTIYDVHYWYAPTATAMVLNMTLPQIYSDPYMQVMGDQCLRELEWAAGASGIFSMVVFAAVT
WFIADIGRKESEDEYISGMQLTDRPAEVNTLLRKNGELSDLRVGDLHMVRMAEVLNFLMHGTIGVGKSTL
IRWLLDYIRKRGDRAIIYDSGCTFTETHYNPSTDFILNAHDERCANWHMWGECVDAVDYDNLAASLIPVE
GESDPFWVSSSRTIFADLAIRMSADPQRSIEKFLKTLLSLSMKSLRDYLANTPSANLVEEKIEKTAISIR
SVVTNYAKALRYLQGLDDGTKPPFTIREWMTQDRYDSSWLFISTQARHRKSVRPLISLWVSLATLMLQSM
GENSERRVWFIIDEAPSLQRIPELAETLAEARKFGGCFVLGMQNMAQLVHVYGRELAKSIFDLMNTRMYG
RSPSAEMAKVVEEELGNQRRRKIREQNSYGLDQVRDGVSLGKDEVNDPVVDYEQIMRLPNLSFYVRLPGE
YPVVRLKLKYRALKKRQAGLMERNIRDALSPELEKVIQENERAATAAGLTFPTGDEVLEKGTEITPAAGS
AAPVSSSGAPAGLTPREEKPVTEPAAGKKTARARPVIRNLRPVTVPVISEVQTTIAESEPDNHREVTAPV
TSSGESNVISLLHGRNSSAGTAPEAPVTDESAPSMSSEPEVAETPAEQATLPAAVQRLRNLTTAPEQPAT
SVLSILQKARQRPAPSESNSGTGDNNGGESMNLEMKVTALPDGGLSVTTAGKPVTPANPDEAHRTNRRLA
QEEENILLHRDAGDPGYDENDQHYYDREPEL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 32768..58215
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| H5H33_RS00170 | 27829..28143 | + | 315 | WP_181726323 | hypothetical protein | - |
| H5H33_RS00175 | 28721..29137 | + | 417 | WP_258559864 | hypothetical protein | - |
| H5H33_RS00180 | 29152..29970 | + | 819 | WP_181726325 | DUF932 domain-containing protein | - |
| H5H33_RS00185 | 29998..30537 | - | 540 | WP_181726300 | lytic transglycosylase domain-containing protein | - |
| H5H33_RS00190 | 31148..31534 | + | 387 | WP_172693770 | relaxosome protein TraM | - |
| H5H33_RS00195 | 31573..32214 | - | 642 | WP_181726301 | Arc family DNA-binding protein | - |
| H5H33_RS00200 | 32411..32764 | + | 354 | WP_172693772 | type IV conjugative transfer system pilin TraA | - |
| H5H33_RS00205 | 32768..33070 | + | 303 | WP_181726302 | type IV conjugative transfer system protein TraL | traL |
| H5H33_RS00210 | 33076..33660 | + | 585 | WP_181726303 | type IV conjugative transfer system protein TraE | traE |
| H5H33_RS00215 | 33672..34412 | + | 741 | WP_181726304 | type-F conjugative transfer system secretin TraK | traK |
| H5H33_RS00220 | 34409..35812 | + | 1404 | WP_181726305 | F-type conjugal transfer pilus assembly protein TraB | traB |
| H5H33_RS00225 | 35823..36287 | + | 465 | WP_181726306 | hypothetical protein | - |
| H5H33_RS00230 | 36256..36717 | + | 462 | WP_181726307 | hypothetical protein | - |
| H5H33_RS00235 | 36748..37143 | + | 396 | WP_172693779 | hypothetical protein | - |
| H5H33_RS00240 | 37201..37764 | + | 564 | WP_181726308 | type IV conjugative transfer system lipoprotein TraV | traV |
| H5H33_RS00245 | 37761..40361 | + | 2601 | WP_172693781 | type IV secretion system protein TraC | virb4 |
| H5H33_RS00250 | 40358..40762 | + | 405 | WP_181726309 | type-F conjugative transfer system protein TrbI | - |
| H5H33_RS00255 | 40759..41394 | + | 636 | WP_148569923 | type-F conjugative transfer system protein TraW | traW |
| H5H33_RS00260 | 41391..41687 | + | 297 | WP_181726310 | hypothetical protein | - |
| H5H33_RS00265 | 41678..42211 | + | 534 | WP_181726311 | hypothetical protein | - |
| H5H33_RS00270 | 42287..42496 | + | 210 | WP_148569926 | hypothetical protein | - |
| H5H33_RS00275 | 42571..43572 | + | 1002 | WP_181726312 | conjugal transfer pilus assembly protein TraU | traU |
| H5H33_RS00280 | 43582..44199 | + | 618 | WP_181726313 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
| H5H33_RS00285 | 44196..46055 | + | 1860 | WP_181726314 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
| H5H33_RS00290 | 46052..46834 | + | 783 | WP_181726315 | type-F conjugative transfer system pilin assembly protein TraF | traF |
| H5H33_RS00295 | 46905..47126 | + | 222 | WP_258559865 | hypothetical protein | - |
| H5H33_RS00300 | 47116..47715 | + | 600 | WP_181726326 | F-type conjugal transfer protein TrbB | traF |
| H5H33_RS00305 | 47733..48002 | + | 270 | WP_181726316 | hypothetical protein | - |
| H5H33_RS00310 | 47995..49359 | + | 1365 | WP_181726317 | conjugal transfer pilus assembly protein TraH | traH |
| H5H33_RS00315 | 49359..52316 | + | 2958 | Protein_63 | conjugal transfer mating-pair stabilization protein TraG | - |
| H5H33_RS00320 | 53928..54485 | + | 558 | WP_181726318 | hypothetical protein | - |
| H5H33_RS00325 | 54646..55377 | + | 732 | WP_181726319 | conjugal transfer complement resistance protein TraT | - |
| H5H33_RS00330 | 55600..58215 | + | 2616 | WP_181726320 | type IV conjugative transfer system coupling protein TraD | virb4 |
Host bacterium
| ID | 15893 | GenBank | NZ_MN842294 |
| Plasmid name | pG426-FII | Incompatibility group | IncFIA |
| Plasmid size | 111767 bp | Coordinate of oriT [Strand] | 30721..30776 [-] |
| Host baterium | Leclercia adecarboxylata strain G426 |
Cargo genes
| Drug resistance gene | mph(A), catB8, ant(3'')-Ia, dfrA1, aac(6')-IIa, blaCARB-12, qacE, sul1, qnrS1 |
| Virulence gene | - |
| Metal resistance gene | merE, merD, merA, merC, merP, merT, merR |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |