Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   115460
Name   oriT_pG426-FII in_silico
Organism   Leclercia adecarboxylata strain G426
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MN842294 (30721..30776 [-], 56 nt)
oriT length   56 nt
IRs (inverted repeats)      16..22, 26..32  (GCAAGAC..GTCTTGC)
Location of nic site      _
Conserved sequence flanking the
  nic site  
 
 _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 56 nt

>oriT_pG426-FII
TTAAAGATAAAATCTGCAAGACAACGTCTTGCGTGGGGTGTGATGTTTTAGATGGT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase


ID   9901 GenBank   WP_181726267
Name   traI_H5H33_RS00335_pG426-FII insolico UniProt ID   _
Length   1810 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1810 a.a.        Molecular weight: 196492.07 Da        Isoelectric Point: 6.2532

>WP_181726267.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Enterobacteriaceae]
MMSVAPVASAGSAAGYYSNSDNYYFLGNLQSLWLGEGAKELGLEGNVRGGDLTAVLEGRLPDGSRLGREI
NGNHVHRPGHDLTFSAPKSVSILGLIGGDKEMVEAHNHAVQVAAGYVEKLISARATKDGITSIVHTGKMV
AAAFTHDTSRNLDPQLHTHLLIANMTEHDGKWKALATDYIHNAGFIETVMKMQVTLGKIYRGALRQRVEA
LGHEVEEVGKHGMWEIKGVPEAVREEFSSRGREIEGAIGAEATLRSRDVAAKDTRQAKVDPSRLRLMERW
LGQMKEKGFDLKAYQESVVPRESGLRDAPPQPDRATPVPASRAEPGAVTRSAPEEKTGKSPESPEVPAER
DDTARQPGSHPADTLRQPSLTDPEKTLVPSAASPARNLHADIPQPELAESVRLAISQLSDARTRFTFGEL
MLTTAELSEQLPEIKDIRVAIDAALKDGMIVPLDSEKGVFTSRIHLLDELSIQALSQEHLKSTKVVSFTR
PAQYAPPALAVAETDPLVLMNAPSGVAGIRDLTVQLTDISAANGRDVQVLASSAERAISLARSDTLRDRL
LSRQHILSGDFHLKPQSTLIIEGAERLGLKETLVLLGEARAQDAQLVFLDSAGRQANGNAMSVLESAGVA
RSRRTEPAPGLEAEVISIANKRDRYEALANRFAELSGGSENVTAVVVGKREQAQLTGLVRDALQNAGQLG
RDGAEIEARQPVWLDSKTRRMPGSYRPGMVLEDRRDAKARKSYVIDRVHDDTRVLSLIDGDGVLTRMKIG
EVSADWRLFSSETLNVATGEKLLAVAGDREHSLKAKDRLEVTAITDKGIEVKRGDDTLTLPKDQPLYVTH
AYVSAPGGRDNDTGVVLAALNTRDISTQTMNSLAQSGHRAEVFTAEVQDRAEARLQRMKTNASPVQLVRS
LSGREDVSEAVGSLHDAVRTDAGLAVWRAVNDQRNVVFSELKLAEAASEYHPDLVAVGNEIAGMVKGGEL
LRVSVRGEPQLIARSTWEMEKAILRTVEAGKNTQQPLLDNVPQAVLNGLTSGQKQSTTLVLGSTDQFTGI
QGYAGVGKTTQIKAVVAALDTLPADARPQLTGLAPTHQAVKEMSDVGVRAQTIKSFIVEHDQAKAAGEKP
DYQGQVFLIDESSMAGNQDTAALFQAIAAGGGRAVSMGDTDQFEAVDSGAPFKLMQERSPMDVAIMKEIV
RQKDVQLRGAVHDIIDNRIGAALKRIEAQPADRIAREAGASLPQSAFEETGSPVNDIVSDWTGRTPDARA
RTLIITQLNADRNAVNAGIHDQLVKRGELGREAVTVPVLDKISHTRHEFNKTAAWDANLVVKRGDRYQDV
LAVDRNGNTVTVRDEEGKIGLYSPKELITGDVQLFRRSQIEVRSGDQLRFTLTDREHGQTGNQRFTVDAV
SQTGDIRLRDERGTVTINPGNVRAQQHIDYAWAVTGYGAQGASTDYVISLEGTEGGRKMLASKRAFYISA
SRAKEHVQIYTDGKADWTKAVAAPERDIKTAHDALAPETQRQQAKAIWAMGQPVTKTAIGRAWVRHQEMG
EASLTAKVIPATRRFPEPALALPVYDNNGKSAGLALVSLVSSPEGRLTQGDVRMVMTERARGAVLQRSQS
GNTVVVNDLAAALDAVRTHPKDGVVWQTGQEPPSANLMKVSGGVREDEGLRLNLSTLTAQDELQRQSELK
LAEQAREQDAVRQQQDDTRAIVLPAEEAVKLRPEELNPEKPAQLIPDNNVLASVRERDQADGRELQLADS
VRKDIAVPDNSAGEERRASRVVTDLARAEQDMVRQAADSGRGRIPEIEEQTLTRTIQKER

  Protein domains


Predicted by InterproScan.

(651-701)

(1029-1219)

(708-786)

(1470-1581)

(11-285)


  Protein structure



No available structure.




T4CP


ID   11541 GenBank   WP_181726320
Name   traD_H5H33_RS00330_pG426-FII insolico UniProt ID   _
Length   871 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 871 a.a.        Molecular weight: 97223.18 Da        Isoelectric Point: 5.1662

>WP_181726320.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacteriaceae]
MSFAGKNLTQGGQMTAYRWRMFLQVNNWIGFWIFILFAALTSAIFFWRIPQETLNNGSLWWFAWMNSSFI
DLMPATGTPTIYDVHYWYAPTATAMVLNMTLPQIYSDPYMQVMGDQCLRELEWAAGASGIFSMVVFAAVT
WFIADIGRKESEDEYISGMQLTDRPAEVNTLLRKNGELSDLRVGDLHMVRMAEVLNFLMHGTIGVGKSTL
IRWLLDYIRKRGDRAIIYDSGCTFTETHYNPSTDFILNAHDERCANWHMWGECVDAVDYDNLAASLIPVE
GESDPFWVSSSRTIFADLAIRMSADPQRSIEKFLKTLLSLSMKSLRDYLANTPSANLVEEKIEKTAISIR
SVVTNYAKALRYLQGLDDGTKPPFTIREWMTQDRYDSSWLFISTQARHRKSVRPLISLWVSLATLMLQSM
GENSERRVWFIIDEAPSLQRIPELAETLAEARKFGGCFVLGMQNMAQLVHVYGRELAKSIFDLMNTRMYG
RSPSAEMAKVVEEELGNQRRRKIREQNSYGLDQVRDGVSLGKDEVNDPVVDYEQIMRLPNLSFYVRLPGE
YPVVRLKLKYRALKKRQAGLMERNIRDALSPELEKVIQENERAATAAGLTFPTGDEVLEKGTEITPAAGS
AAPVSSSGAPAGLTPREEKPVTEPAAGKKTARARPVIRNLRPVTVPVISEVQTTIAESEPDNHREVTAPV
TSSGESNVISLLHGRNSSAGTAPEAPVTDESAPSMSSEPEVAETPAEQATLPAAVQRLRNLTTAPEQPAT
SVLSILQKARQRPAPSESNSGTGDNNGGESMNLEMKVTALPDGGLSVTTAGKPVTPANPDEAHRTNRRLA
QEEENILLHRDAGDPGYDENDQHYYDREPEL

  Protein domains


Predicted by InterproScan.

(32-136)

(182-569)

  Protein structure



No available structure.




T4SS


T4SS were predicted by using oriTfinder2.

Region 1: 32768..58215

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H5H33_RS00170 27829..28143 + 315 WP_181726323 hypothetical protein -
H5H33_RS00175 28721..29137 + 417 WP_258559864 hypothetical protein -
H5H33_RS00180 29152..29970 + 819 WP_181726325 DUF932 domain-containing protein -
H5H33_RS00185 29998..30537 - 540 WP_181726300 lytic transglycosylase domain-containing protein -
H5H33_RS00190 31148..31534 + 387 WP_172693770 relaxosome protein TraM -
H5H33_RS00195 31573..32214 - 642 WP_181726301 Arc family DNA-binding protein -
H5H33_RS00200 32411..32764 + 354 WP_172693772 type IV conjugative transfer system pilin TraA -
H5H33_RS00205 32768..33070 + 303 WP_181726302 type IV conjugative transfer system protein TraL traL
H5H33_RS00210 33076..33660 + 585 WP_181726303 type IV conjugative transfer system protein TraE traE
H5H33_RS00215 33672..34412 + 741 WP_181726304 type-F conjugative transfer system secretin TraK traK
H5H33_RS00220 34409..35812 + 1404 WP_181726305 F-type conjugal transfer pilus assembly protein TraB traB
H5H33_RS00225 35823..36287 + 465 WP_181726306 hypothetical protein -
H5H33_RS00230 36256..36717 + 462 WP_181726307 hypothetical protein -
H5H33_RS00235 36748..37143 + 396 WP_172693779 hypothetical protein -
H5H33_RS00240 37201..37764 + 564 WP_181726308 type IV conjugative transfer system lipoprotein TraV traV
H5H33_RS00245 37761..40361 + 2601 WP_172693781 type IV secretion system protein TraC virb4
H5H33_RS00250 40358..40762 + 405 WP_181726309 type-F conjugative transfer system protein TrbI -
H5H33_RS00255 40759..41394 + 636 WP_148569923 type-F conjugative transfer system protein TraW traW
H5H33_RS00260 41391..41687 + 297 WP_181726310 hypothetical protein -
H5H33_RS00265 41678..42211 + 534 WP_181726311 hypothetical protein -
H5H33_RS00270 42287..42496 + 210 WP_148569926 hypothetical protein -
H5H33_RS00275 42571..43572 + 1002 WP_181726312 conjugal transfer pilus assembly protein TraU traU
H5H33_RS00280 43582..44199 + 618 WP_181726313 type-F conjugative transfer system pilin assembly protein TrbC trbC
H5H33_RS00285 44196..46055 + 1860 WP_181726314 type-F conjugative transfer system mating-pair stabilization protein TraN traN
H5H33_RS00290 46052..46834 + 783 WP_181726315 type-F conjugative transfer system pilin assembly protein TraF traF
H5H33_RS00295 46905..47126 + 222 WP_258559865 hypothetical protein -
H5H33_RS00300 47116..47715 + 600 WP_181726326 F-type conjugal transfer protein TrbB traF
H5H33_RS00305 47733..48002 + 270 WP_181726316 hypothetical protein -
H5H33_RS00310 47995..49359 + 1365 WP_181726317 conjugal transfer pilus assembly protein TraH traH
H5H33_RS00315 49359..52316 + 2958 Protein_63 conjugal transfer mating-pair stabilization protein TraG -
H5H33_RS00320 53928..54485 + 558 WP_181726318 hypothetical protein -
H5H33_RS00325 54646..55377 + 732 WP_181726319 conjugal transfer complement resistance protein TraT -
H5H33_RS00330 55600..58215 + 2616 WP_181726320 type IV conjugative transfer system coupling protein TraD virb4


Host bacterium


ID   15893 GenBank   NZ_MN842294
Plasmid name   pG426-FII Incompatibility group   IncFIA
Plasmid size   111767 bp Coordinate of oriT [Strand]   30721..30776 [-]
Host baterium   Leclercia adecarboxylata strain G426

Cargo genes


Drug resistance gene   mph(A), catB8, ant(3'')-Ia, dfrA1, aac(6')-IIa, blaCARB-12, qacE, sul1, qnrS1
Virulence gene   -
Metal resistance gene   merE, merD, merA, merC, merP, merT, merR
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -