Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 115435 |
| Name | oriT_pJH1  |
| Organism | Staphylococcus aureus strain JH4322 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP133661 (7415..7450 [-], 36 nt) |
| oriT length | 36 nt |
| IRs (inverted repeats) | 4..10, 14..20 (GCGAACG..CGTTCGC) |
| Location of nic site | 29..30 |
| Conserved sequence flanking the nic site |
GTGCGCCCTT |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 36 nt
>oriT_pJH1
CACGCGAACGGAACGTTCGCATAAGTGCGCCCTTAC
CACGCGAACGGAACGTTCGCATAAGTGCGCCCTTAC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 9881 | GenBank | WP_001252101 |
| Name | MobA_MobL_RGC87_RS14455_pJH1 |
UniProt ID | A0A141HMI9 |
| Length | 665 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 665 a.a. Molecular weight: 78908.86 Da Isoelectric Point: 5.3910
>WP_001252101.1 MULTISPECIES: MobA/MobL family protein [Bacilli]
MAMYHFQNKFVSKANGQSATAKSAYNSASRIKDFKENEFKDYSNKQCDYSEILLPNNADDKFKDREYLWN
KVHDVENRKNSQVAREIIIGLPNEFDPNSNIELAKEFAESLSNEGMIVDLNIHKINEENPHAHLLCTLRG
LDKNNEFEPKRKGNDYIRDWNTKEKHNEWRKRWENVQNKHLEKNGFSVRVSADSYKNQNIDLEPTKKEGW
KARKFEDETGKKSSISKHNESIKKRNQQKIDKMFNEVDDMKSHKLNAFSYMNKSDSTTLKNMAKDLKIYV
TPINMYKENERLYDLKQKTSLITDDEDRLNKIEDIEDRQKKLESINEVFEKQAGIFFDKNYPDQSLNYSD
DEKIFITRTILNDRDVLPANNELEDIVKEKRIKEAQISLNTVLGNRDISLESIEKESNFFADKLSNILEK
NNLSFDDVLENKHEGMEDSLKIDYYTNKLEVFRNAENILEDYYDVQIKELFTDDEDYKAFNEVTDIKEKQ
QLIDFKTYHGTENTIEMLETGNFIPKYSDEDRKYITEQVKLLQEKEFKPNKNQHDKFVFGAIQKKLLSEY
DFDYSDNNDLKHLYQESNEVGDEISKDNIEEFYEGNDILVDKETYNNYNRKQQAYGLINAGLDSMIFNFN
EIFRERMPKYINHQYKGKNHSKQRHELKNKRGMHL
MAMYHFQNKFVSKANGQSATAKSAYNSASRIKDFKENEFKDYSNKQCDYSEILLPNNADDKFKDREYLWN
KVHDVENRKNSQVAREIIIGLPNEFDPNSNIELAKEFAESLSNEGMIVDLNIHKINEENPHAHLLCTLRG
LDKNNEFEPKRKGNDYIRDWNTKEKHNEWRKRWENVQNKHLEKNGFSVRVSADSYKNQNIDLEPTKKEGW
KARKFEDETGKKSSISKHNESIKKRNQQKIDKMFNEVDDMKSHKLNAFSYMNKSDSTTLKNMAKDLKIYV
TPINMYKENERLYDLKQKTSLITDDEDRLNKIEDIEDRQKKLESINEVFEKQAGIFFDKNYPDQSLNYSD
DEKIFITRTILNDRDVLPANNELEDIVKEKRIKEAQISLNTVLGNRDISLESIEKESNFFADKLSNILEK
NNLSFDDVLENKHEGMEDSLKIDYYTNKLEVFRNAENILEDYYDVQIKELFTDDEDYKAFNEVTDIKEKQ
QLIDFKTYHGTENTIEMLETGNFIPKYSDEDRKYITEQVKLLQEKEFKPNKNQHDKFVFGAIQKKLLSEY
DFDYSDNNDLKHLYQESNEVGDEISKDNIEEFYEGNDILVDKETYNNYNRKQQAYGLINAGLDSMIFNFN
EIFRERMPKYINHQYKGKNHSKQRHELKNKRGMHL
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A141HMI9 |
T4CP
| ID | 11519 | GenBank | WP_000209436 |
| Name | t4cp2_RGC87_RS14590_pJH1 |
UniProt ID | _ |
| Length | 546 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 546 a.a. Molecular weight: 62672.60 Da Isoelectric Point: 7.6569
>WP_000209436.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Bacilli]
MTTLLGELESKATSINKKNLVIQDFDTKCPNRNIFVVGGPGSYKSAGYVIPNVIVKNQQSIVVTDPSGEV
YEKTSNIKRMQGFDVRVVNFKNFLASDRQNFFDYIDKDSDCSIVAELIIKSAGDSKINKDVWYKASVGLL
NSLLLYAKYEFEPEKRTIGNIIKFIQNNKPNQDEEGSVELDKRFNELSKDHPARESYEFGFAVSEGRTRA
SIILTFVADLRNYIDNEIRSYTSDSDFDLRDVGLRKTIIYVMLPVLGNTVQSLSSLFFSQMFQQLYRLGD
ENGARLPVPVDFLLDEWPNIGAIPDYEETLATCRKYGISITTIVQSISQAVDKYNKDKANAIIGNHAVIL
CLGNVNNDTAKYIKEELGNATVEFETSSEGSSSGKDSRSKSSNKNVSYTGRALLNEDEISNMQQDKAILI
TKNRNPKIIKKLAYFKMFPNLTETYIAPQNEYKRKKNQSMIDKHNRLREEWDKKQEKNKQQKLEEYKEEQ
RQKELEKEQQPQEEQQNEKVKESKDKNDQEDKKDEQQKINDSKKAFYEKLKQKQAK
MTTLLGELESKATSINKKNLVIQDFDTKCPNRNIFVVGGPGSYKSAGYVIPNVIVKNQQSIVVTDPSGEV
YEKTSNIKRMQGFDVRVVNFKNFLASDRQNFFDYIDKDSDCSIVAELIIKSAGDSKINKDVWYKASVGLL
NSLLLYAKYEFEPEKRTIGNIIKFIQNNKPNQDEEGSVELDKRFNELSKDHPARESYEFGFAVSEGRTRA
SIILTFVADLRNYIDNEIRSYTSDSDFDLRDVGLRKTIIYVMLPVLGNTVQSLSSLFFSQMFQQLYRLGD
ENGARLPVPVDFLLDEWPNIGAIPDYEETLATCRKYGISITTIVQSISQAVDKYNKDKANAIIGNHAVIL
CLGNVNNDTAKYIKEELGNATVEFETSSEGSSSGKDSRSKSSNKNVSYTGRALLNEDEISNMQQDKAILI
TKNRNPKIIKKLAYFKMFPNLTETYIAPQNEYKRKKNQSMIDKHNRLREEWDKKQEKNKQQKLEEYKEEQ
RQKELEKEQQPQEEQQNEKVKESKDKNDQEDKKDEQQKINDSKKAFYEKLKQKQAK
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 30567..41788
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| RGC87_RS14545 (RGC87_14545) | 25630..26004 | + | 375 | Protein_30 | IS256 family transposase | - |
| RGC87_RS14550 (RGC87_14550) | 26061..26453 | + | 393 | WP_000393259 | GNAT family N-acetyltransferase | - |
| RGC87_RS14555 (RGC87_14555) | 26454..27737 | + | 1284 | Protein_32 | aminoglycoside N-acetyltransferase AAC(6')-Ie | - |
| RGC87_RS14560 (RGC87_14560) | 27739..28740 | - | 1002 | Protein_33 | IS256-like element IS256 family transposase | - |
| RGC87_RS14565 (RGC87_14565) | 28787..29161 | - | 375 | Protein_34 | IS256 family transposase | - |
| RGC87_RS14570 (RGC87_14570) | 29222..29896 | - | 675 | WP_001105984 | IS6-like element IS257 family transposase | - |
| RGC87_RS14575 (RGC87_14575) | 29934..30101 | - | 168 | WP_000869301 | hypothetical protein | - |
| RGC87_RS14580 (RGC87_14580) | 30158..30550 | - | 393 | WP_000608970 | single-stranded DNA-binding protein | - |
| RGC87_RS14585 (RGC87_14585) | 30567..31484 | - | 918 | WP_000383804 | conjugal transfer protein TrbL family protein | trsL |
| RGC87_RS14590 (RGC87_14590) | 31562..33202 | - | 1641 | WP_000209436 | VirD4-like conjugal transfer protein, CD1115 family | - |
| RGC87_RS14595 (RGC87_14595) | 33199..33663 | - | 465 | WP_000715273 | hypothetical protein | trsJ |
| RGC87_RS14600 (RGC87_14600) | 33679..35781 | - | 2103 | WP_001094109 | DNA topoisomerase III | - |
| RGC87_RS14605 (RGC87_14605) | 35797..36282 | - | 486 | WP_000735569 | TrsH/TraH family protein | - |
| RGC87_RS14610 (RGC87_14610) | 36292..37368 | - | 1077 | WP_000269385 | CHAP domain-containing protein | trsG |
| RGC87_RS14615 (RGC87_14615) | 37386..38666 | - | 1281 | WP_000702361 | membrane protein | trsF |
| RGC87_RS14620 (RGC87_14620) | 38678..40690 | - | 2013 | WP_309480399 | helicase HerA domain-containing protein | virb4 |
| RGC87_RS14625 (RGC87_14625) | 40711..41394 | - | 684 | WP_000979860 | TrsD/TraD family conjugative transfer protein | trsD |
| RGC87_RS14630 (RGC87_14630) | 41381..41788 | - | 408 | WP_000110157 | hypothetical protein | trsC |
| RGC87_RS14635 (RGC87_14635) | 41848..42165 | - | 318 | WP_000591622 | CagC family type IV secretion system protein | - |
Host bacterium
| ID | 15868 | GenBank | NZ_CP133661 |
| Plasmid name | pJH1 | Incompatibility group | - |
| Plasmid size | 42831 bp | Coordinate of oriT [Strand] | 7415..7450 [-] |
| Host baterium | Staphylococcus aureus strain JH4322 |
Cargo genes
| Drug resistance gene | mupA, qacD, aph(2'')-Ia |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIA21 |