Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   115430
Name   oriT_p32 in_silico
Organism   Enterococcus faecium strain 515
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP041255 (40063..40255 [+], 193 nt)
oriT length   193 nt
IRs (inverted repeats)      123..128, 142..147  (AAAATA..TATTTT)
 123..128, 131..136  (AAAATA..TATTTT)
 104..111, 122..129  (ATATTTTT..AAAAATAT)
 109..114, 121..126  (TTTTTG..CAAAAA)
 65..71, 75..81  (AGTTGGC..GCCAACT)
 41..48, 53..60  (CACCTTCC..GGAAGGTG)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 193 nt

>oriT_p32
CCAAGGAATTAATACAAAGAGCATAAGGGAAAACTGATAGCACCTTCCCAAAGGAAGGTGGCTAAGTTGGCTGTGCCAACTGGTTTTCTTTCAAAATCACCTCATATTTTTTTGCTATTACAAAAATATCTATTTTCGACCTATTTTCGGCCATAATATAGTACCTACTTTTGGTCATAGTTTCGTCCGTAGT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   9876 GenBank   WP_002326870
Name   mobP2_FKZ20_RS00625_p32 insolico UniProt ID   B5U8V6
Length   506 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 506 a.a.        Molecular weight: 60597.01 Da        Isoelectric Point: 9.3485

>WP_002326870.1 MULTISPECIES: MobP2 family relaxase [Enterococcus]
MTEHIFNRKSASIILKAKFETGKSKKNNHMVELQKFVDYISRQEAIRQDKLDHDYSEDELNELARIEKAL
EKIEQETDQPVIKDLREMDKYIDYMTRKKAILENVDNEIVNGAFSNTKRYITKKDISKIKESVIEAKNNG
SVMFQDVISFDNDFLVREGYYNPETNELNENVLYEATKSMMGKMQEKEELVDPFWFATIHRNTEHIHIHV
TAMERKNTREIMEYDGVLQARGKRKQSTLDDMIFKFGSKILDRTNEFEKISKLRKEVPLELKQSVKDSLI
QLYVENNSRYDKELVKYLKELKKEIPSTTRGYNELPEETKEKIDEVTNYMTKDNPKRKEYDRMTKEIDEL
YQTTYGKRYNPQEYYLNRKKDFNARMGNAVVGQIKLLKQSEEKNLKSNELKNLLDGKKLNVKFDREKPEF
KTKYAKQSYDDFQKRLEERQLAREKNEVHFKKISEKFKDKKLIRKIDRVINDDLNMYRAKNDYEVAQRMI
EQEKMRQAREFEIGMN

  Protein domains


Predicted by InterproScan.

MobL relaxases (MobL)

(11-438)


  Protein structure


Source ID Structure
AlphaFold DB B5U8V6


T4CP

ID   11513 GenBank   WP_033795274
Name   t4cp2_FKZ20_RS00690_p32 insolico UniProt ID   _
Length   952 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 952 a.a.        Molecular weight: 108268.06 Da        Isoelectric Point: 8.1346

>WP_033795274.1 VirD4-like conjugal transfer protein, CD1115 family [Enterococcus faecium]
MKLVQNSEVKKRKNGFAKNSNKILVKLKKAGSLKKNKYHTGKIEGEDLPKSLQDKLNVPLSFLGIFLGSI
VFLAITYLLNFLKSLLVAIGQSESGLLGVEFDPKFSLTHLRPLGDTKQYFMCAVVVVIGVAYLIYGKLNH
ESEKNLVYGQKGDSRLSTIKEIKRDYHEIPEKTEVYDGIGGVPISHYQDKYYIDRDTVNTVVLGASRSGK
GETFVVPLLDNLSRAKIQSNMVVNDPKGELFSASKETLERRGYRVLVLNIDDPLESMSFNPLQLVIDSWA
NKDYHEASKRANTLTSMLFASGMGTDNEFFYKSAKSAVNAIILTIVEHCFNNDCIEKITMYNVAQMLNEL
GSLFYTDPKTGKEKNALDEYFNTLPQGNQAKIQYGSTSFAGDKAKGSILSTASQGIEMFTSDLFGKLTSK
MSIDLKEIGFPKSIQFKVNTNLVGKRISISFLRRENDVIRLIKKYRVKVKALGLCVLNFNEYLQDGDMLD
IRYEEEKSRAWYRIEFPKKQANSHDTSLVTKKFKSGANFSDLEISTLRLKYTDQPTAVFMVIPDYDPSNH
VLASIFISQLYTELASNCKQTPDKKCFRRVHFLLDEFGNMPAIDNMDGIMTVCLGRNMLFDLVIQSYSQL
ETRYDKAFKTIKENCQNHILIMSNDEETIEEISKKCGHKTTINRSASSKHLDTDSSVTSSAEQERVITVE
RASQLIEGEQIILRNLHRQDTKRNKIRPFPIFNTKETNMPYRYQFLAEDFDTQRDINEIDIACEHINLSL
QDNQIPYAKFIKDLKTRLEYSIINNIPISDEDYHNYRNLSGSQQTINEEELLKLVNTEEKNQDTEGQVLS
EQEMKINIYLKESQKEAIELVKVINGRLADENSADMVIKTINSQVLDTPFIQSLINDSIAIHNAKENKDQ
IMILEQQLANAYELAVLKTKNSILKNKIIKLKNAMTKLVKAI

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(589-701)

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(199-443)

  Protein structure



No available structure.




Host bacterium

ID   15863 GenBank   NZ_CP041255
Plasmid name   p32 Incompatibility group   -
Plasmid size   53447 bp Coordinate of oriT [Strand]   40063..40255 [+]
Host baterium   Enterococcus faecium strain 515

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIA1