Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 115427
• Name: oriT_pUTI-056-1
• Organism: Staphylococcus saprophyticus strain UTI-056
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP054445 (8795..8959 [-], 165 nt)
• oriT length: 165 nt
• IRs (inverted repeats): 143..148, 156..161 (AGCATA..TATGCT) 126..134, 136..144 (CTATCATTT..AAATGATAG) 109..115, 119..125 (GTCTGGC..GCCAGAC)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 165 nt

>oriT_pUTI-056-1
CTATATATAGTGTCACAAAGGTGAGACACCCACTATATACAGTGTCACAAAGGTGAGACAAAAGGGGATTTAAGATATCATTTAAAAATTGACTTGAATTGTTGGAATGTCTGGCTTCGCCAGACCTATCATTTTAAATGATAGCATATTCCCCTTATGCTCTTA

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobP2_HSZ51_RS12710_pUTI-056-1

• ID: 9873
• GenBank: WP_031884043
• Name: mobP2_HSZ51_RS12710_pUTI-056-1
• UniProt ID: A0A6H0A0Z5
• Length: 391 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 391 a.a.
• Molecular weight: 47185.96 Da
• Isoelectric Point: 9.0873

>WP_031884043.1 MULTISPECIES: MobP2 family relaxase [Staphylococcus]
MPKIVTMSEFEQSKNKTKFSQYVDYVDRDEAKKNEHKEFQYDVYTHYMFDEQKSNSMFNDNSNYMSEDEI
KYTKSKFNEAQKNNGIMWKDVISFDNESLKEAGIFDPECKYLDEQKMRQATRKAMQKFEKKEGLENNLVW
SSSIHYNTDNIHVHVAAVEKDVTRERGKRKYSTIKEMKSSFANELFDMSGERTKINNFIRDRIVKGIKEQ
NEPDYDKQMKLQLKKIHEEIKDIPKKEWQYNNNIMKQARPEIDKFTLMYIKNNHGEEFDEFKQDLKKQSK
LYKETYGDKSDYKKYEETKMNDLYSRAGNTVLKQIKSFNEEYNNIKFKEINKQDNKKPLNINPKVNNKMQ
IGYALNSTLYNTQRALRNDFQKERNINQYHYEFDKSYEKEE

Protein domains

Predicted by InterproScan.

• MobL relaxases (MobL) (1-368)

Protein structure

• AlphaFold DB: A0A6H0A0Z5

T4CP

t4cp2_HSZ51_RS12760_pUTI-056-1

• ID: 11510
• GenBank: WP_156981573
• Name: t4cp2_HSZ51_RS12760_pUTI-056-1
• UniProt ID: _
• Length: 941 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 941 a.a.
• Molecular weight: 108575.68 Da
• Isoelectric Point: 8.3855

>WP_156981573.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Staphylococcus]
MIPFRSKHNHGYHRKKASFSQNKHLLAHPIGLLALLVVGFIFIMLAANFFINMLNNLIFAGGDIFDNMKK
INNEWTNYFFKVRFDLALFYIPFFVLLTVLWGKKVYQIRQKFKSLDNQEKASGRFSTKKEIKQQYKAIAQ
REKQFEGEGGLPVAMFGVYDFATKTKQYLEQRKTAKTEINSMPDLTQDEKYNMKKDKLGQINKEFVSTFF
LKREFTFIDESPTNNIFVGTSRSGKGEVFVLSMIENYSRSSHQPSLVVGDMKGELYSASNETLENRNYHT
EVFNLDQPMESTMSFNLLQLVIEEYQKGDLGEAQEMTKQITHTLTNNEGVEDNEWNLMSAALINAMILAL
CEEALPEHPEKVTLYSVSNMLQTLSVKKFYKQIQIGNQVKVIETSALDEYMEKFPSHSPAKTQYATVEAA
PDKMRSSIIGTALKALQPFTTDTIAKLTSHSSLDLNKLGFPSIIDGFSEPDSKFELNVQVKRETNIGTKY
DEVEGINIGVNEYGYWQYPFKTVLNIDDRIEIVETDEFNEEVKYYFYVTSIDKKGQVTFEAKGDIYSSNV
TIRKFNSFAKPIAIFMVVPDYNSANHGIASILVNQIVFELSKNSQLYTEKQSTHRRVIFHLDEAGNMPQI
PNLSQKVNVSLSRGLRFNFFVQAFSQIKDTYGDAYDAIMDACQNKVFIMATQEQTLKDFSDMIGSQQITV
RSRSGETDSLKSSITENQEERPLLRADELAHLQEGETVVVRNLKRQDNKRRKVMSYPIFNSGKYAMKYRY
QYLADLMDTGKSIIHFRPLLKERCEHRHLQLETTLVDWEKIIENMQAGIENDSNNDIDDLTQSLNKSMGE
KANANNPEVYKEENNKKPVTRQKKVLTLKAQLSAEEFDRLTRRFTKTIRMYEKDGNNVDRLTLYKLKQYL
KLRVRNNDITEEQKNKSINYVKQLIEEAKAK

Protein domains

Predicted by InterproScan.

• Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf) (190-459)

• TraM recognition site of TraD and TraG (TraG-D_C) (616-733)

Protein structure

No available structure.

Host bacterium

• ID: 15860
• GenBank: NZ_CP054445
• Plasmid name: pUTI-056-1
• Incompatibility group: -
• Plasmid size: 39196 bp
• Coordinate of oriT [Strand]: 8795..8959 [-]
• Host baterium: Staphylococcus saprophyticus strain UTI-056

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: AcrIIA21